GLOD4_HUMAN
ID GLOD4_HUMAN Reviewed; 313 AA.
AC Q9HC38; D3DTG9; D3DTH1; Q96B89; Q9H3J8; Q9HC37; Q9NVN1;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Glyoxalase domain-containing protein 4;
GN Name=GLOD4; Synonyms=C17orf25; ORFNames=CGI-150, My027;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), AND TISSUE SPECIFICITY.
RX PubMed=11642406; DOI=10.1038/sj.cr.7290088;
RA Qin W.X., Wan D.F., Sun F.Y., Zhang P.P., Han L.W., Huang Y., Jiang H.Q.,
RA Zhao X.T., He M., Ye Y., Cong W.M., Wu M.C., Zhang L.S., Yang N.W.,
RA Gu J.R.;
RT "Cloning and characterization of a novel gene (C17orf25) from the deletion
RT region on chromosome 17p13.3 in hepatocellular carcinoma.";
RL Cell Res. 11:209-216(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Fetal brain;
RA Mao Y.M., Xie Y., Ying K.;
RL Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT ILE-282.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PROTEIN SEQUENCE OF 6-12; 97-116; 156-177; 188-198; 240-252 AND 289-305,
RP AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Fetal brain cortex;
RA Lubec G., Chen W.-Q., Sun Y.;
RL Submitted (DEC-2008) to UniProtKB.
RN [7]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH NUDT9.
RX PubMed=12897971;
RA Zhang H.-T., Yan Z.-Q., Hu X.-B., Yang S.-L., Gong Y.;
RT "Interaction of C17orf25 with ADP-ribose pyrophosphatase NUDT9 detected via
RT yeast two-hybrid method.";
RL Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 35:747-751(2003).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- SUBUNIT: Interacts with NUDT9. {ECO:0000269|PubMed:12897971}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305|PubMed:12897971}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9HC38-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9HC38-2; Sequence=VSP_023646;
CC Name=3;
CC IsoId=Q9HC38-3; Sequence=VSP_023646, VSP_023647, VSP_023648;
CC -!- TISSUE SPECIFICITY: Expressed in heart, brain, liver, kidney, pancreas
CC and placenta. Not expressed in skeletal muscle and lung.
CC {ECO:0000269|PubMed:11642406}.
CC -!- MISCELLANEOUS: Expression is decreased in hepatocellular carcinoma
CC samples as compared to adjacent non-cancerous liver tissues from the
CC same patients. Transfection in hepatocellular carcinoma cells and
CC overexpression can inhibit the cell growth.
CC -!- SIMILARITY: Belongs to the glyoxalase I family. {ECO:0000305}.
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DR EMBL; AF177342; AAG17986.1; -; mRNA.
DR EMBL; AF177343; AAG17987.1; -; mRNA.
DR EMBL; AF061730; AAG43141.1; -; mRNA.
DR EMBL; AK001488; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; CH471108; EAW90645.1; -; Genomic_DNA.
DR EMBL; CH471108; EAW90646.1; -; Genomic_DNA.
DR EMBL; CH471108; EAW90647.1; -; Genomic_DNA.
DR EMBL; CH471108; EAW90649.1; -; Genomic_DNA.
DR EMBL; CH471108; EAW90650.1; -; Genomic_DNA.
DR EMBL; BC008605; AAH08605.1; -; mRNA.
DR EMBL; BC015848; AAH15848.1; -; mRNA.
DR CCDS; CCDS32520.1; -. [Q9HC38-2]
DR RefSeq; NP_057164.3; NM_016080.3. [Q9HC38-2]
DR PDB; 3ZI1; X-ray; 1.90 A; A=1-287.
DR PDBsum; 3ZI1; -.
DR AlphaFoldDB; Q9HC38; -.
DR SMR; Q9HC38; -.
DR BioGRID; 119237; 68.
DR IntAct; Q9HC38; 9.
DR MINT; Q9HC38; -.
DR ChEMBL; CHEMBL4295951; -.
DR GlyGen; Q9HC38; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q9HC38; -.
DR MetOSite; Q9HC38; -.
DR PhosphoSitePlus; Q9HC38; -.
DR SwissPalm; Q9HC38; -.
DR BioMuta; GLOD4; -.
DR DMDM; 74718831; -.
DR REPRODUCTION-2DPAGE; IPI00792035; -.
DR EPD; Q9HC38; -.
DR jPOST; Q9HC38; -.
DR MassIVE; Q9HC38; -.
DR MaxQB; Q9HC38; -.
DR PeptideAtlas; Q9HC38; -.
DR PRIDE; Q9HC38; -.
DR ProteomicsDB; 81633; -. [Q9HC38-1]
DR ProteomicsDB; 81634; -. [Q9HC38-2]
DR ProteomicsDB; 81635; -. [Q9HC38-3]
DR TopDownProteomics; Q9HC38-2; -. [Q9HC38-2]
DR Antibodypedia; 10285; 116 antibodies from 25 providers.
DR DNASU; 51031; -.
DR Ensembl; ENST00000301328.9; ENSP00000301328.5; ENSG00000167699.14. [Q9HC38-1]
DR Ensembl; ENST00000301329.11; ENSP00000301329.6; ENSG00000167699.14. [Q9HC38-2]
DR GeneID; 51031; -.
DR KEGG; hsa:51031; -.
DR MANE-Select; ENST00000301329.11; ENSP00000301329.6; NM_016080.4; NP_057164.3. [Q9HC38-2]
DR UCSC; uc002fru.4; human. [Q9HC38-1]
DR CTD; 51031; -.
DR DisGeNET; 51031; -.
DR GeneCards; GLOD4; -.
DR HGNC; HGNC:14111; GLOD4.
DR HPA; ENSG00000167699; Low tissue specificity.
DR neXtProt; NX_Q9HC38; -.
DR OpenTargets; ENSG00000167699; -.
DR PharmGKB; PA162389795; -.
DR VEuPathDB; HostDB:ENSG00000167699; -.
DR GeneTree; ENSGT00390000012340; -.
DR HOGENOM; CLU_044479_0_0_1; -.
DR InParanoid; Q9HC38; -.
DR OMA; GCEAACN; -.
DR OrthoDB; 824414at2759; -.
DR PhylomeDB; Q9HC38; -.
DR TreeFam; TF105801; -.
DR PathwayCommons; Q9HC38; -.
DR SignaLink; Q9HC38; -.
DR BioGRID-ORCS; 51031; 14 hits in 1079 CRISPR screens.
DR ChiTaRS; GLOD4; human.
DR GeneWiki; GLOD4; -.
DR GenomeRNAi; 51031; -.
DR Pharos; Q9HC38; Tbio.
DR PRO; PR:Q9HC38; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q9HC38; protein.
DR Bgee; ENSG00000167699; Expressed in buccal mucosa cell and 202 other tissues.
DR ExpressionAtlas; Q9HC38; baseline and differential.
DR Genevisible; Q9HC38; HS.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0045296; F:cadherin binding; HDA:BHF-UCL.
DR CDD; cd16357; GLOD4_C; 1.
DR Gene3D; 3.10.180.10; -; 2.
DR InterPro; IPR043193; GLOD4.
DR InterPro; IPR043194; GLOD4_C.
DR InterPro; IPR029068; Glyas_Bleomycin-R_OHBP_Dase.
DR InterPro; IPR037523; VOC.
DR PANTHER; PTHR46466; PTHR46466; 1.
DR SUPFAM; SSF54593; SSF54593; 2.
DR PROSITE; PS51819; VOC; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Direct protein sequencing;
KW Mitochondrion; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..313
FT /note="Glyoxalase domain-containing protein 4"
FT /id="PRO_0000280391"
FT DOMAIN 5..145
FT /note="VOC 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01163"
FT DOMAIN 152..273
FT /note="VOC 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01163"
FT MOD_RES 146
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9CPV4"
FT MOD_RES 288
FT /note="N6-succinyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q9CPV4"
FT VAR_SEQ 32..62
FT /note="ESCSVARLECSGAISAHCSDYTRITEDSFSK -> LRHEEFEEGCKAACNG
FT (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:11642406,
FT ECO:0000303|PubMed:14702039, ECO:0000303|PubMed:15489334,
FT ECO:0000303|Ref.2"
FT /id="VSP_023646"
FT VAR_SEQ 197..203
FT /note="CKLELQG -> VSNLGEE (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_023647"
FT VAR_SEQ 204..313
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_023648"
FT VARIANT 282
FT /note="M -> I (in dbSNP:rs17851349)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_031128"
FT CONFLICT 295
FT /note="A -> S (in Ref. 1; AAG17987)"
FT /evidence="ECO:0000305"
FT STRAND 5..12
FT /evidence="ECO:0007829|PDB:3ZI1"
FT HELIX 16..25
FT /evidence="ECO:0007829|PDB:3ZI1"
FT STRAND 30..37
FT /evidence="ECO:0007829|PDB:3ZI1"
FT STRAND 68..76
FT /evidence="ECO:0007829|PDB:3ZI1"
FT TURN 78..80
FT /evidence="ECO:0007829|PDB:3ZI1"
FT STRAND 83..89
FT /evidence="ECO:0007829|PDB:3ZI1"
FT STRAND 99..107
FT /evidence="ECO:0007829|PDB:3ZI1"
FT HELIX 109..118
FT /evidence="ECO:0007829|PDB:3ZI1"
FT STRAND 123..126
FT /evidence="ECO:0007829|PDB:3ZI1"
FT STRAND 129..133
FT /evidence="ECO:0007829|PDB:3ZI1"
FT STRAND 139..145
FT /evidence="ECO:0007829|PDB:3ZI1"
FT STRAND 152..161
FT /evidence="ECO:0007829|PDB:3ZI1"
FT HELIX 163..172
FT /evidence="ECO:0007829|PDB:3ZI1"
FT STRAND 177..182
FT /evidence="ECO:0007829|PDB:3ZI1"
FT TURN 183..186
FT /evidence="ECO:0007829|PDB:3ZI1"
FT STRAND 187..193
FT /evidence="ECO:0007829|PDB:3ZI1"
FT STRAND 198..203
FT /evidence="ECO:0007829|PDB:3ZI1"
FT STRAND 216..221
FT /evidence="ECO:0007829|PDB:3ZI1"
FT HELIX 223..225
FT /evidence="ECO:0007829|PDB:3ZI1"
FT HELIX 226..235
FT /evidence="ECO:0007829|PDB:3ZI1"
FT STRAND 239..247
FT /evidence="ECO:0007829|PDB:3ZI1"
FT STRAND 255..261
FT /evidence="ECO:0007829|PDB:3ZI1"
FT STRAND 267..272
FT /evidence="ECO:0007829|PDB:3ZI1"
FT HELIX 273..279
FT /evidence="ECO:0007829|PDB:3ZI1"
SQ SEQUENCE 313 AA; 34793 MW; 43DB04159153F07C CRC64;
MAARRALHFV FKVGNRFQTA RFYRDVLGMK VESCSVARLE CSGAISAHCS DYTRITEDSF
SKPYDGKWSK TMVGFGPEDD HFVAELTYNY GVGDYKLGND FMGITLASSQ AVSNARKLEW
PLTEVAEGVF ETEAPGGYKF YLQNRSLPQS DPVLKVTLAV SDLQKSLNYW CNLLGMKIYE
KDEEKQRALL GYADNQCKLE LQGVKGGVDH AAAFGRIAFS CPQKELPDLE DLMKRENQKI
LTPLVSLDTP GKATVQVVIL ADPDGHEICF VGDEAFRELS KMDPEGSKLL DDAMAADKSD
EWFAKHNKPK ASG
