GLOD4_MOUSE
ID GLOD4_MOUSE Reviewed; 298 AA.
AC Q9CPV4; Q3UUS8; Q9CY26; Q9D9F5;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Glyoxalase domain-containing protein 4;
GN Name=Glod4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Embryo, Liver, Testis, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-109 AND LYS-273, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
CC -!- SUBUNIT: Interacts with NUDT9. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9CPV4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9CPV4-2; Sequence=VSP_023650, VSP_023651;
CC Name=3;
CC IsoId=Q9CPV4-3; Sequence=VSP_023649;
CC -!- SIMILARITY: Belongs to the glyoxalase I family. {ECO:0000305}.
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DR EMBL; AK006979; BAB24818.1; -; mRNA.
DR EMBL; AK010992; BAB27311.1; -; mRNA.
DR EMBL; AK012569; BAB28324.1; -; mRNA.
DR EMBL; AK013212; BAB28716.1; -; mRNA.
DR EMBL; AK138073; BAE23546.1; -; mRNA.
DR EMBL; AL591129; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC061012; AAH61012.1; -; mRNA.
DR CCDS; CCDS25063.1; -. [Q9CPV4-1]
DR CCDS; CCDS88193.1; -. [Q9CPV4-3]
DR RefSeq; NP_080305.2; NM_026029.3. [Q9CPV4-1]
DR RefSeq; XP_006534033.1; XM_006533970.3.
DR AlphaFoldDB; Q9CPV4; -.
DR SMR; Q9CPV4; -.
DR BioGRID; 212012; 2.
DR IntAct; Q9CPV4; 1.
DR STRING; 10090.ENSMUSP00000017430; -.
DR iPTMnet; Q9CPV4; -.
DR PhosphoSitePlus; Q9CPV4; -.
DR SwissPalm; Q9CPV4; -.
DR REPRODUCTION-2DPAGE; Q9CPV4; -.
DR CPTAC; non-CPTAC-3812; -.
DR EPD; Q9CPV4; -.
DR jPOST; Q9CPV4; -.
DR MaxQB; Q9CPV4; -.
DR PaxDb; Q9CPV4; -.
DR PeptideAtlas; Q9CPV4; -.
DR PRIDE; Q9CPV4; -.
DR ProteomicsDB; 266812; -. [Q9CPV4-1]
DR ProteomicsDB; 266813; -. [Q9CPV4-2]
DR ProteomicsDB; 266814; -. [Q9CPV4-3]
DR Antibodypedia; 10285; 116 antibodies from 25 providers.
DR DNASU; 67201; -.
DR Ensembl; ENSMUST00000017430; ENSMUSP00000017430; ENSMUSG00000017286. [Q9CPV4-1]
DR Ensembl; ENSMUST00000170710; ENSMUSP00000131788; ENSMUSG00000017286. [Q9CPV4-3]
DR GeneID; 67201; -.
DR KEGG; mmu:67201; -.
DR UCSC; uc007kfc.2; mouse. [Q9CPV4-2]
DR UCSC; uc007kfl.2; mouse. [Q9CPV4-1]
DR CTD; 51031; -.
DR MGI; MGI:1914451; Glod4.
DR VEuPathDB; HostDB:ENSMUSG00000017286; -.
DR eggNOG; KOG2943; Eukaryota.
DR GeneTree; ENSGT00390000012340; -.
DR InParanoid; Q9CPV4; -.
DR OMA; GCEAACN; -.
DR OrthoDB; 824414at2759; -.
DR PhylomeDB; Q9CPV4; -.
DR TreeFam; TF105801; -.
DR BioGRID-ORCS; 67201; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Glod4; mouse.
DR PRO; PR:Q9CPV4; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q9CPV4; protein.
DR Bgee; ENSMUSG00000017286; Expressed in ureteric bud tip and 262 other tissues.
DR ExpressionAtlas; Q9CPV4; baseline and differential.
DR Genevisible; Q9CPV4; MM.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR CDD; cd16357; GLOD4_C; 1.
DR Gene3D; 3.10.180.10; -; 2.
DR InterPro; IPR043193; GLOD4.
DR InterPro; IPR043194; GLOD4_C.
DR InterPro; IPR029068; Glyas_Bleomycin-R_OHBP_Dase.
DR InterPro; IPR037523; VOC.
DR PANTHER; PTHR46466; PTHR46466; 1.
DR SUPFAM; SSF54593; SSF54593; 1.
DR PROSITE; PS51819; VOC; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Mitochondrion; Phosphoprotein; Reference proteome;
KW Repeat.
FT CHAIN 1..298
FT /note="Glyoxalase domain-containing protein 4"
FT /id="PRO_0000280392"
FT DOMAIN 5..130
FT /note="VOC 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01163"
FT DOMAIN 137..258
FT /note="VOC 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01163"
FT MOD_RES 109
FT /note="N6-succinyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
FT MOD_RES 131
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 273
FT /note="N6-succinyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
FT VAR_SEQ 1..30
FT /note="MATRRALHFVFKVKNRFQTVHFFRDVLGMQ -> MICGSVLNSPD (in
FT isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_023649"
FT VAR_SEQ 278..291
FT /note="AMEADKSDEWFATR -> GEGCTDRVRTANPG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_023650"
FT VAR_SEQ 292..298
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_023651"
FT CONFLICT 46
FT /note="N -> Y (in Ref. 1; BAB27311)"
FT /evidence="ECO:0000305"
FT CONFLICT 84
FT /note="N -> K (in Ref. 1; BAB24818)"
FT /evidence="ECO:0000305"
FT CONFLICT 214
FT /note="L -> I (in Ref. 1; BAB27311)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 298 AA; 33317 MW; F2CD5FD2387A78CE CRC64;
MATRRALHFV FKVKNRFQTV HFFRDVLGMQ VLRHEEFEEG CKAACNGPYD GKWSKTMVGF
GPEDDHFVAE LTYNYGIGDY KLGNDFMGIT LASSQAVSNA RKLEWPLSKV AEGIFETEAP
GGYKFYLQDR SPSQSDPVLK VTLAVSDLQK SLNYWSNLLG MKIYEQDEEK QRALLGYADN
QCKLELQGIQ GAVDHAAAFG RIAFSCPQKE LPDLEDLMKR ESHSILTPLV SLDTPGKATV
QVVILADPDG HEICFVGDEA FRELSKMDPK GSKLLDDAME ADKSDEWFAT RNKPKASG
