ID   GLOX_VITPS              Reviewed;         523 AA.
AC   Q3HRQ2;
DT   08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Aldehyde oxidase GLOX {ECO:0000305};
DE            EC=1.2.3.1 {ECO:0000305};
DE   AltName: Full=Glyoxal oxidase {ECO:0000303|Ref.1};
DE            Short=VpGLOX {ECO:0000303|PubMed:20512385};
DE   Flags: Precursor;
GN   Name=GLOX {ECO:0000303|PubMed:23090239};
OS   Vitis pseudoreticulata (Chinese wild grapevine).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; Vitales; Vitaceae; Viteae; Vitis.
OX   NCBI_TaxID=231512;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION BY THE GRAPEVINE POWDERY MILDEW.
RC   TISSUE=Leaf;
RA   Zhou B.J., Wang X.P., Wang Y.J.;
RT   "cDNA cloning, expression, protein purification, and characterization of a
RT   novel glyoxal oxidase related gene from Vitis pseudoreticulata.";
RL   Biol. Plant. 51:458-466(2007).
RN   [2]
RP   FUNCTION.
RX   PubMed=20512385; DOI=10.1007/s00709-010-0162-4;
RA   Guan X., Zhao H., Xu Y., Wang Y.;
RT   "Transient expression of glyoxal oxidase from the Chinese wild grape Vitis
RT   pseudoreticulata can suppress powdery mildew in a susceptible genotype.";
RL   Protoplasma 248:415-423(2011).
RN   [3]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=23090239; DOI=10.1007/s00709-012-0462-y;
RA   Zhao H., Guan X., Xu Y., Wang Y.;
RT   "Characterization of novel gene expression related to glyoxal oxidase by
RT   agro-infiltration of the leaves of accession Baihe-35-1 of Vitis
RT   pseudoreticulata involved in production of H2O2 for resistance to Erysiphe
RT   necator.";
RL   Protoplasma 250:765-777(2013).
CC   -!- FUNCTION: Catalyzes the oxidation of aldehydes to the corresponding
CC       carboxylate by coupling the reaction to the reduction of dioxygen to
CC       hydrogen peroxide. Substrates include glyoxal and other aldehydes (By
CC       similarity). Involved in disease resistance against the grapevine
CC       powdery mildew E.necator. Is sufficient to confer disease resistance to
CC       E.necator (PubMed:20512385, PubMed:23090239). Can produce hydrogen
CC       peroxide in response to E.necator infection, and this may directly play
CC       a role in the defense mechanism during plant-pathogen interactions
CC       (PubMed:23090239). {ECO:0000250|UniProtKB:Q01772,
CC       ECO:0000269|PubMed:20512385, ECO:0000269|PubMed:23090239}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an aldehyde + H2O + O2 = a carboxylate + H(+) + H2O2;
CC         Xref=Rhea:RHEA:16829, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:16240, ChEBI:CHEBI:17478,
CC         ChEBI:CHEBI:29067; EC=1.2.3.1; Evidence={ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC       {ECO:0000269|PubMed:23090239}.
CC   -!- INDUCTION: Induced by infection by the grapevine powdery mildew
CC       E.necator. {ECO:0000269|Ref.1}.
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DR   EMBL; DQ201181; ABA42922.1; -; mRNA.
DR   AlphaFoldDB; Q3HRQ2; -.
DR   SMR; Q3HRQ2; -.
DR   PRIDE; Q3HRQ2; -.
DR   GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR   GO; GO:0004031; F:aldehyde oxidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050832; P:defense response to fungus; IMP:UniProtKB.
DR   CDD; cd02851; E_set_GO_C; 1.
DR   Gene3D; 2.130.10.80; -; 1.
DR   Gene3D; 2.60.40.10; -; 1.
DR   InterPro; IPR011043; Gal_Oxase/kelch_b-propeller.
DR   InterPro; IPR037293; Gal_Oxidase_central_sf.
DR   InterPro; IPR009880; Glyoxal_oxidase_N.
DR   InterPro; IPR015202; GO-like_E_set.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   Pfam; PF09118; DUF1929; 1.
DR   Pfam; PF07250; Glyoxal_oxid_N; 1.
DR   SUPFAM; SSF50965; SSF50965; 1.
DR   SUPFAM; SSF81296; SSF81296; 1.
PE   2: Evidence at transcript level;
KW   Cell wall; Oxidoreductase; Plant defense; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..523
FT                   /note="Aldehyde oxidase GLOX"
FT                   /id="PRO_0000436454"
SQ   SEQUENCE   523 AA;  57319 MW;  35667B2BC504792D CRC64;
     MILDAAIVAL ADLPGTWELI VPNAGIASMH TAVTRYGTVV LLDRTNIGPS RKMLPKGHCR
     YDPKDEVLKR DCYAHSVILD LNTNKIRPLK ILTDTWCSSG QFLPDGSLLQ TGGDLDGVKK
     IRKFVPCGPH GFCDWEELKD VELETGRWYA TNQILPDGSV IIVGGRAANS VEYYPPRKGG
     AVQLPFLSDV EDKQMDNLYP YVHLLPNGHL FIFANNKAVM YDYTSNKVML EYPPLDGGPR
     NYPSAGSSVM LALEGDYSMA IIVVCGGAQF GAFIQKSTDT PAHGSCGRIV ATSPHPVWEM
     EDMPFGRIMG DMVMLPTGDV LIINGAQAGS QGFELASSPC FFPLLYRPNQ PLGLRFMTLT
     PGTVPRMYHS TANLLPDGRV LIAGSNPHYF YKFAAEFPTE LRIEAFSPEY LFADKANIRP
     VIDESPEMVR FGEQFDVFVS VSLPVVGSME VNLASAPFAT HSFSQGQRLV KLTVSPTVPD
     ADERYRIVCT APPGGKIAPP GYYMMFAVNL GVPSVARWVQ LVP