ID   GLP1_BRANA              Reviewed;         207 AA.
AC   P46271;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Germin-like protein 1;
DE   Flags: Precursor;
GN   Name=GER1;
OS   Brassica napus (Rape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Samourai; TISSUE=Seedling;
RX   PubMed=9349269; DOI=10.1023/a:1005833028582;
RA   Membre N., Berna A., Neutelings G., David A., David H., Staiger D.,
RA   Saez Vasquez J., Raynal M., Delseny M., Bernier F.;
RT   "cDNA sequence, genomic organization and differential expression of three
RT   Arabidopsis genes for germin/oxalate oxidase-like proteins.";
RL   Plant Mol. Biol. 35:459-469(1997).
CC   -!- FUNCTION: May play a role in plant defense. Probably has no oxalate
CC       oxidase activity even if the active site is conserved.
CC   -!- SUBUNIT: Oligomer (believed to be a pentamer but probably hexamer).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the germin family. {ECO:0000305}.
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DR   EMBL; U21743; AAA86365.1; -; mRNA.
DR   PIR; T07854; T07854.
DR   RefSeq; NP_001302724.1; NM_001315795.1.
DR   AlphaFoldDB; P46271; -.
DR   SMR; P46271; -.
DR   GeneID; 106354228; -.
DR   KEGG; bna:106354228; -.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR   Gene3D; 2.60.120.10; -; 1.
DR   InterPro; IPR006045; Cupin_1.
DR   InterPro; IPR001929; Germin.
DR   InterPro; IPR019780; Germin_Mn-BS.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF00190; Cupin_1; 1.
DR   PRINTS; PR00325; GERMIN.
DR   SMART; SM00835; Cupin_1; 1.
DR   SUPFAM; SSF51182; SSF51182; 1.
DR   PROSITE; PS00725; GERMIN; 1.
PE   2: Evidence at transcript level;
KW   Apoplast; Disulfide bond; Glycoprotein; Manganese; Metal-binding; Secreted;
KW   Signal.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255"
FT   CHAIN           18..207
FT                   /note="Germin-like protein 1"
FT                   /id="PRO_0000010836"
FT   DOMAIN          51..197
FT                   /note="Cupin type-1"
FT                   /evidence="ECO:0000255"
FT   BINDING         99
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         101
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         106
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         145
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        58
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        23..38
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   207 AA;  21514 MW;  B6A29465A73B956E CRC64;
     MLRIIFLLSL LFALSNDSVQ DFCVANLKRA ETPAGYPCIR PIHVKASDFV FSLGTPGNTT
     NIISAAVTPG FVAQFPALNG LGISTARLDL APKGVIPMHT HPGASEVLFV LDGSITAGFI
     SSANSVYVQT LKPGQVMVFP QGLLHFQINA GKTPAAALVT FSSASPGLQI LDFALFANTL
     STELVSATTF LPPATVKTLK GVLGGTG