ID   GLP1_IPONI              Reviewed;         214 AA.
AC   P45853;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Germin-like protein;
DE   Flags: Precursor;
GN   Name=GLP;
OS   Ipomoea nil (Japanese morning glory) (Pharbitis nil).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Convolvulaceae; Ipomoeeae; Ipomoea.
OX   NCBI_TaxID=35883;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Choisy; TISSUE=Cotyledon;
RX   PubMed=8888623; DOI=10.1093/oxfordjournals.pcp.a029022;
RA   Ono M., Sage-Ono K., Inoue M., Kamada H., Harada H.;
RT   "Transient increase in the level of mRNA for a germin-like protein in
RT   leaves of the short-day plant Pharbitis nil during the photoperiodic
RT   induction of flowering.";
RL   Plant Cell Physiol. 37:855-861(1996).
CC   -!- SUBUNIT: Oligomer (believed to be a pentamer but probably hexamer).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Cotyledons and leaves.
CC   -!- DEVELOPMENTAL STAGE: Increased transiently during flower induction.
CC   -!- INDUCTION: Expressed with a circadian rhythm, with peak expression 10
CC       hours from the beginning of the night.
CC   -!- SIMILARITY: Belongs to the germin family. {ECO:0000305}.
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DR   EMBL; D45425; BAA08266.1; -; mRNA.
DR   AlphaFoldDB; P45853; -.
DR   SMR; P45853; -.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR   Gene3D; 2.60.120.10; -; 1.
DR   InterPro; IPR006045; Cupin_1.
DR   InterPro; IPR001929; Germin.
DR   InterPro; IPR019780; Germin_Mn-BS.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF00190; Cupin_1; 1.
DR   PRINTS; PR00325; GERMIN.
DR   SMART; SM00835; Cupin_1; 1.
DR   SUPFAM; SSF51182; SSF51182; 1.
DR   PROSITE; PS00725; GERMIN; 1.
PE   2: Evidence at transcript level;
KW   Apoplast; Disulfide bond; Manganese; Metal-binding; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..214
FT                   /note="Germin-like protein"
FT                   /id="PRO_0000010838"
FT   DOMAIN          58..204
FT                   /note="Cupin type-1"
FT                   /evidence="ECO:0000255"
FT   BINDING         106
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         108
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         113
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   DISULFID        28..44
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   214 AA;  22608 MW;  2BF187AA8CCAC4AC CRC64;
     MVMMRIFFFL FLLAFPVFTA NASVNDFCVA NGPGARDTPS GFVCKNTAKV TAADFVYSGL
     AKPGNTTNII NAAVTPAFVG QFPGVIGLGV TLARLDLAPG GVIPMHTHPG ASEILNVVEG
     TILAAFISSG NKVYEKALYP GDVMVFPQGL LDFQEITGKI APGLCELRQR NPGLQILEFQ
     HCFPTIFPPK TIAGTTCLDE ATIKKLKSGL GGTN