GLP1_MORAL
ID GLP1_MORAL Reviewed; 209 AA.
AC L8BRS3; B3EWH8;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2013, sequence version 1.
DT 03-AUG-2022, entry version 18.
DE RecName: Full=Germin-like protein {ECO:0000303|PubMed:23284650};
DE Short=Ma-Glp {ECO:0000303|PubMed:23284650};
DE Flags: Precursor;
OS Morus alba (White mulberry).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Moraceae; Morus.
OX NCBI_TaxID=3498;
RN [1] {ECO:0000305, ECO:0000312|EMBL:CCH57381.3}
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-36, FUNCTION,
RP BIOPHYSICOCHEMICAL PROPERTIES, AND MASS SPECTROMETRY.
RC TISSUE=Leaf {ECO:0000312|EMBL:CCH57381.3};
RX PubMed=23284650; DOI=10.1371/journal.pone.0050900;
RA Patnaik B.B., Kim D.H., Oh S.H., Song Y.S., Chanh N.D., Kim J.S.,
RA Jung W.J., Saha A.K., Bindroo B.B., Han Y.S.;
RT "Molecular cloning and characterization of novel Morus alba germin-like
RT protein gene which encodes for a silkworm gut digestion-resistant
RT antimicrobial protein.";
RL PLoS ONE 7:E50900-E50900(2012).
CC -!- FUNCTION: Has antibacterial activity against B.subtilis (MIC=5 ug),
CC B.cereus (MIC=50 ug), A.hydrophila (MIC=2.5 ug), S.marcescens(MIC=10
CC ug), S.enterica (MIC=10 ug), P.entomophila (MIC=2.5 ug) and P.rhodesiae
CC (MIC=10 ug). Has antifungal activity against F.solani KACC 40384 and
CC F.oxysporum KACC 40032. Probably has no oxalate oxidase activity even
CC if the active site is conserved. {ECO:0000250|UniProtKB:P94072,
CC ECO:0000269|PubMed:23284650}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Antibacterial activity retained between pH 7 and 10.
CC {ECO:0000269|PubMed:23284650};
CC Temperature dependence:
CC Antibacterial activity retained between 10 and 70 degrees Celsius.
CC {ECO:0000269|PubMed:23284650};
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000250|UniProtKB:P45850}.
CC -!- MASS SPECTROMETRY: Mass=21285; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:23284650};
CC -!- SIMILARITY: Belongs to the germin family. {ECO:0000255}.
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DR EMBL; HE805964; CCH57381.3; -; mRNA.
DR AlphaFoldDB; L8BRS3; -.
DR SMR; L8BRS3; -.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR006045; Cupin_1.
DR InterPro; IPR001929; Germin.
DR InterPro; IPR019780; Germin_Mn-BS.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR Pfam; PF00190; Cupin_1; 1.
DR PRINTS; PR00325; GERMIN.
DR SMART; SM00835; Cupin_1; 1.
DR SUPFAM; SSF51182; SSF51182; 1.
DR PROSITE; PS00725; GERMIN; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Apoplast; Direct protein sequencing;
KW Disulfide bond; Fungicide; Glycoprotein; Manganese; Metal-binding;
KW Receptor; Secreted; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000269|PubMed:23284650"
FT CHAIN 19..209
FT /note="Germin-like protein"
FT /evidence="ECO:0000269|PubMed:23284650"
FT /id="PRO_0000422700"
FT DOMAIN 53..199
FT /note="Cupin type-1"
FT /evidence="ECO:0000255"
FT BINDING 101
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:P45850"
FT BINDING 103
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:P45850"
FT BINDING 108
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:P45850"
FT BINDING 147
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250|UniProtKB:P45850"
FT CARBOHYD 60
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 24..39
FT /evidence="ECO:0000250|UniProtKB:P45850"
SQ SEQUENCE 209 AA; 21888 MW; 3FB6E79A33FC4003 CRC64;
MIVPIFFLFS LLFSSSHGAI QDFCVADYSA PQGPAGYSCK NPAKVTVDNF VYSGLGITGN
TTNLIKAAVT TAFDNQFPGV NGLGISLARP DLAPGGVIPF HTHPGASEII IVIEGSLCAA
FVSSDNKVYL KSLKKGDTMI FPSGLLHFQL NAGKNNALFF VAFNSPNPGL QLVDYALFGN
DLATELVAAA SFLDPAEIKR LKAVLGGSG
