ID   AMDS_ASPOR              Reviewed;         545 AA.
AC   Q12559; Q2U364;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2006, sequence version 2.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=Acetamidase;
DE            EC=3.5.1.4;
GN   Name=amdS; ORFNames=AO090038000171;
OS   Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=510516;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 42149 / RIB 40;
RX   PubMed=1840550; DOI=10.1016/0378-1119(91)90491-s;
RA   Gomi K., Kitamoto K., Kumagai C.;
RT   "Cloning and molecular characterization of the acetamidase-encoding gene
RT   (amdS) from Aspergillus oryzae.";
RL   Gene 108:91-98(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 42149 / RIB 40;
RX   PubMed=16372010; DOI=10.1038/nature04300;
RA   Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA   Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA   Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA   Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA   Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA   Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA   Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA   Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA   Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA   Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA   Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA   Kikuchi H.;
RT   "Genome sequencing and analysis of Aspergillus oryzae.";
RL   Nature 438:1157-1161(2005).
CC   -!- FUNCTION: Allows acetamide to be used as a sole carbon or nitrogen
CC       source.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a monocarboxylic acid amide + H2O = a monocarboxylate +
CC         NH4(+); Xref=Rhea:RHEA:12020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:35757, ChEBI:CHEBI:83628; EC=3.5.1.4;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetamide + H2O = acetate + NH4(+); Xref=Rhea:RHEA:45048,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:27856, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:30089; EC=3.5.1.4;
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAE64001.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; D10492; BAA01373.1; -; Genomic_DNA.
DR   EMBL; AP007169; BAE64001.1; ALT_SEQ; Genomic_DNA.
DR   PIR; JS0633; JS0633.
DR   RefSeq; XP_001825134.2; XM_001825082.2.
DR   AlphaFoldDB; Q12559; -.
DR   SMR; Q12559; -.
DR   STRING; 510516.Q12559; -.
DR   EnsemblFungi; BAE64001; BAE64001; AO090038000171.
DR   Proteomes; UP000006564; Chromosome 6.
DR   GO; GO:0004040; F:amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0043864; F:indoleacetamide hydrolase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..545
FT                   /note="Acetamidase"
FT                   /id="PRO_0000105128"
FT   ACT_SITE        130
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        205
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        229
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        247
FT                   /note="G -> D (in Ref. 1; BAA01373)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   545 AA;  60098 MW;  F0686F4C08752EE3 CRC64;
     MPSASWEDLA ADKRARLEKS IPDEWKFKSV PIEGSVIDLP EKSGILSPSE IKITNSSATE
     LVAQLANGTL KSVDVTLAFC KRAALAHQLV NCAHDFFPEL ALAQARELDR YFETHKKPVG
     PLHGLPISLK DQLRVKGTET CMAYISWLGK RDTSDSILTA LLRKAGAVFL VKTSVPQTLM
     VCETVNNIIG RTSNPRNLNL SCGGSSGGEG AMIAMRGGAI GIGTDIGGSI RVPAAFNSLY
     GIRPSHGRLP YGGMTNSMEG QETIHSVVGP IAHSAQDVRL FLQSVLKEEP WKYDSKVIPL
     PWREAEENAA QAKIAEKSLN FAFYDFDGVV RPHPPITRGV EIVRSTLEKD GHTVAPWTPY
     KHAFAVDLAN KIYAADGSTD VYKHINASGE PAIPNIKDLM NPNLPKADLN EVWDAQLQKW
     RYQCEYLDKW REWEERTGKE LDAIIAPVAA TAAVRHNQFR YYGYATVFNV LDYTSVVVPV
     TYADKAVDHR LADYQPVSDM DKAVYAEYDP EVYHGAPVAV QIIGRRLSEE RTLAIAEYVG
     KLLGH