AMDY_YEAST
ID AMDY_YEAST Reviewed; 549 AA.
AC P22580; D6VSM2;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Probable amidase;
DE EC=3.5.1.4;
GN Name=AMD2; Synonyms=AMDY, AMDY1; OrderedLocusNames=YDR242W;
GN ORFNames=YD8419.09;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2263500; DOI=10.1093/nar/18.23.7180;
RA Chang T.-H., Abelson J.;
RT "Identification of a putative amidase gene in yeast Saccharomyces
RT cerevisiae.";
RL Nucleic Acids Res. 18:7180-7180(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a monocarboxylic acid amide + H2O = a monocarboxylate +
CC NH4(+); Xref=Rhea:RHEA:12020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:35757, ChEBI:CHEBI:83628; EC=3.5.1.4;
CC -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
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DR EMBL; X56043; CAA39514.1; -; Genomic_DNA.
DR EMBL; Z49701; CAA89728.1; -; Genomic_DNA.
DR EMBL; AY723784; AAU09701.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12082.1; -; Genomic_DNA.
DR PIR; S54538; S54538.
DR RefSeq; NP_010528.3; NM_001180550.3.
DR AlphaFoldDB; P22580; -.
DR SMR; P22580; -.
DR BioGRID; 32293; 74.
DR MINT; P22580; -.
DR STRING; 4932.YDR242W; -.
DR MaxQB; P22580; -.
DR PaxDb; P22580; -.
DR PRIDE; P22580; -.
DR EnsemblFungi; YDR242W_mRNA; YDR242W; YDR242W.
DR GeneID; 851829; -.
DR KEGG; sce:YDR242W; -.
DR SGD; S000002650; AMD2.
DR VEuPathDB; FungiDB:YDR242W; -.
DR eggNOG; KOG1212; Eukaryota.
DR HOGENOM; CLU_009600_9_2_1; -.
DR InParanoid; P22580; -.
DR OMA; SMMATET; -.
DR BioCyc; YEAST:YDR242W-MON; -.
DR PRO; PR:P22580; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; P22580; protein.
DR GO; GO:0004040; F:amidase activity; IEA:UniProtKB-EC.
DR GO; GO:0043864; F:indoleacetamide hydrolase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.90.1300.10; -; 1.
DR InterPro; IPR020556; Amidase_CS.
DR InterPro; IPR023631; Amidase_dom.
DR InterPro; IPR036928; AS_sf.
DR Pfam; PF01425; Amidase; 1.
DR SUPFAM; SSF75304; SSF75304; 1.
DR PROSITE; PS00571; AMIDASES; 1.
PE 3: Inferred from homology;
KW Hydrolase; Reference proteome.
FT CHAIN 1..549
FT /note="Probable amidase"
FT /id="PRO_0000105130"
FT ACT_SITE 132
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 209
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 233
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000250"
FT CONFLICT 525
FT /note="G -> S (in Ref. 1; CAA39514)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 549 AA; 61410 MW; E7CB049845C1FCDC CRC64;
MTVHSTWKEK VQLKKDQLNS KIKDEWKLNS TTITRLKNDK KNLIKNIDDL CSSSENQITH
STIMALRQAL EAKELSCHEI TAAFCHRAAL IHQVVNCLSE IMFSEALRLA DYYDSNRPAI
LPPLYGIPIS LKDQCNVEGV DTSLGYLCRT FKPKTKNEES LIVSFLRDLG AIIFVKTTVP
SSMMATDTQS NTFGYTYNSI NLSFSSGGSS GGEGSLIGAH GSLLGLGTDI GGSIRIPSSY
QGLFGLKPTF GRVPYLRVDN SFEGRETIPS VIGPLARDLS DLRYFMSCVI NICQPWVQDV
KCIPYHFDSS TSKLHDNYVV GIWYGDGVID PPPSDIRALK TCEDLVNKTK GMKAVKWEPS
SELSRELFDL ANEADVADSG NEIKNEFEIS GEPLLDILKP MVLENGRPPY TVNEWWDLTK
RVYNAQQLMR DYYLSFPESE RPDVIISPTT LMPFRPGDML KTTLRYILLF NVLNFPSLSI
PVGSVDCQID GLMDTTSALN PEDKMIKTYW NDLIQSGEID GFPIGLQVVS PTFNDNEVCK
FASWLFSKI
