GLPD_PSEAE
ID GLPD_PSEAE Reviewed; 512 AA.
AC P52111;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Glycerol-3-phosphate dehydrogenase;
DE EC=1.1.5.3;
GN Name=glpD; OrderedLocusNames=PA3584;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=CD10;
RX PubMed=8157588; DOI=10.1128/jb.176.8.2184-2193.1994;
RA Schweizer H.P., Po C.;
RT "Cloning and nucleotide sequence of the glpD gene encoding sn-glycerol-3-
RT phosphate dehydrogenase of Pseudomonas aeruginosa.";
RL J. Bacteriol. 176:2184-2193(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinone + sn-glycerol 3-phosphate = a quinol +
CC dihydroxyacetone phosphate; Xref=Rhea:RHEA:18977, ChEBI:CHEBI:24646,
CC ChEBI:CHEBI:57597, ChEBI:CHEBI:57642, ChEBI:CHEBI:132124; EC=1.1.5.3;
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the FAD-dependent glycerol-3-phosphate
CC dehydrogenase family. {ECO:0000305}.
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DR EMBL; L06231; AAA81584.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG06972.1; -; Genomic_DNA.
DR PIR; A55207; A55207.
DR PIR; B83197; B83197.
DR RefSeq; NP_252274.1; NC_002516.2.
DR RefSeq; WP_003098460.1; NZ_QZGE01000001.1.
DR AlphaFoldDB; P52111; -.
DR SMR; P52111; -.
DR STRING; 287.DR97_4355; -.
DR PaxDb; P52111; -.
DR PRIDE; P52111; -.
DR EnsemblBacteria; AAG06972; AAG06972; PA3584.
DR GeneID; 880179; -.
DR KEGG; pae:PA3584; -.
DR PATRIC; fig|208964.12.peg.3751; -.
DR PseudoCAP; PA3584; -.
DR HOGENOM; CLU_015740_5_0_6; -.
DR InParanoid; P52111; -.
DR OMA; CIVNAAG; -.
DR PhylomeDB; P52111; -.
DR BioCyc; PAER208964:G1FZ6-3653-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
DR GO; GO:0004368; F:glycerol-3-phosphate dehydrogenase (quinone) activity; IBA:GO_Central.
DR GO; GO:0052591; F:sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity; IEA:UniProtKB-EC.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0046168; P:glycerol-3-phosphate catabolic process; IBA:GO_Central.
DR Gene3D; 1.10.8.870; -; 1.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR031656; DAO_C.
DR InterPro; IPR038299; DAO_C_sf.
DR InterPro; IPR006076; FAD-dep_OxRdtase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR000447; G3P_DH_FAD-dep.
DR PANTHER; PTHR11985; PTHR11985; 1.
DR Pfam; PF01266; DAO; 1.
DR Pfam; PF16901; DAO_C; 1.
DR PRINTS; PR01001; FADG3PDH.
DR SUPFAM; SSF51905; SSF51905; 1.
DR PROSITE; PS00977; FAD_G3PDH_1; 1.
DR PROSITE; PS00978; FAD_G3PDH_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; FAD; Flavoprotein; Glycerol metabolism; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..512
FT /note="Glycerol-3-phosphate dehydrogenase"
FT /id="PRO_0000126104"
FT BINDING 16..44
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255"
FT CONFLICT 77..78
FT /note="EA -> DT (in Ref. 1; AAA81584)"
FT /evidence="ECO:0000305"
FT CONFLICT 133..140
FT /note="GLRFTGSS -> ACVHRQQ (in Ref. 1; AAA81584)"
FT /evidence="ECO:0000305"
FT CONFLICT 180..208
FT /note="RTRCVSARRSKGLWHLHLERSDGSLYSIR -> APAASAPVAARDSGTCTWS
FT AATAACIRS (in Ref. 1; AAA81584)"
FT /evidence="ECO:0000305"
FT CONFLICT 425..430
FT /note="LRWART -> PALGAH (in Ref. 1; AAA81584)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 512 AA; 57135 MW; DFF734821ABD730C CRC64;
MSQAHTPSAP LAEVYDVAVV GGGINGVGIA ADAAGRGLSV FLCEQHDLAQ HTSSASSKLI
HGGLRYLEHY EFRLVREALA EREVLLAKAP HIVKPLRFVL PHRPHLRPAW MIRAGLFLYD
HLGKREKLPA SRGLRFTGSS PLKAEIRRGF EYSDCAVDDA RLVVLNAISA REHGAHVHTR
TRCVSARRSK GLWHLHLERS DGSLYSIRAR ALVNAAGPWV ARFIQDDLKQ KSPYGIRLIQ
GSHIIVPKLY EGEHAYILQN EDRRIVFAIP YLDRFTMIGT TDREYQGDPA KVAISEEETA
YLLQVVNAHF KQQLAAADIL HSFAGVRPLC DDESDEPSAI TRDYTLSLSA GNGEPPLLSV
FGGKLTTYRK LAESALTQLQ PFFANLGPAW TAKAPLPGGE QMQSVEALTE QLANRYAWLD
RELALRWART YGTRVWRLLD GVNGEADLGE HLGGGLYARE VDYLCKHEWA QDAEDILWRR
SKLGLFLSPS QQVRLGQYLQ SEHPHRPRVH AA
