GLPF4_LACPL
ID GLPF4_LACPL Reviewed; 238 AA.
AC F9UMX3;
DT 27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT 19-OCT-2011, sequence version 1.
DT 03-AUG-2022, entry version 58.
DE RecName: Full=Glycerol uptake facilitator protein 4 {ECO:0000303|PubMed:23799297};
DE AltName: Full=D/L-lactic acid transporter {ECO:0000303|PubMed:23799297};
DE AltName: Full=Lactic acid channel {ECO:0000303|PubMed:23799297};
GN Name=glpF4 {ECO:0000303|PubMed:23799297, ECO:0000312|EMBL:CCC78562.1};
GN OrderedLocusNames=lp_1175 {ECO:0000312|EMBL:CCC78562.1};
OS Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
OS (Lactobacillus plantarum).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactiplantibacillus.
OX NCBI_TaxID=220668;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX PubMed=12566566; DOI=10.1073/pnas.0337704100;
RA Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P.,
RA Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J.,
RA Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M.,
RA Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M.,
RA Siezen R.J.;
RT "Complete genome sequence of Lactobacillus plantarum WCFS1.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX PubMed=22156394; DOI=10.1128/jb.06275-11;
RA Siezen R.J., Francke C., Renckens B., Boekhorst J., Wels M.,
RA Kleerebezem M., van Hijum S.A.;
RT "Complete resequencing and reannotation of the Lactobacillus plantarum
RT WCFS1 genome.";
RL J. Bacteriol. 194:195-196(2012).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX PubMed=23799297; DOI=10.1042/bj20130388;
RA Bienert G.P., Desguin B., Chaumont F., Hols P.;
RT "Channel-mediated lactic acid transport: a novel function for
RT aquaglyceroporins in bacteria.";
RL Biochem. J. 454:559-570(2013).
CC -!- FUNCTION: Transporter that facilitates the transmembrane diffusion of
CC water, dihydroxyacetone, glycerol, urea, H(2)O(2) and D/L-lactic acid.
CC Is involved in the cellular racemization of lactate and lactate
CC metabolism, but has likely a more general physiological role. The
CC transported molecule is indeed lactic acid and not the lactate anion,
CC in agreement with the assumption that, with very few exceptions, MIPs
CC (major intrinsic proteins) only facilitate the transport of uncharged
CC solutes. {ECO:0000269|PubMed:23799297}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23799297};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene show a decreased lactate
CC transport ability. {ECO:0000269|PubMed:23799297}.
CC -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
CC {ECO:0000305}.
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DR EMBL; AL935263; CCC78562.1; -; Genomic_DNA.
DR RefSeq; WP_003643315.1; NC_004567.2.
DR RefSeq; YP_004889076.1; NC_004567.2.
DR AlphaFoldDB; F9UMX3; -.
DR SMR; F9UMX3; -.
DR STRING; 220668.lp_1175; -.
DR EnsemblBacteria; CCC78562; CCC78562; lp_1175.
DR GeneID; 57024809; -.
DR GeneID; 66449064; -.
DR KEGG; lpl:lp_1175; -.
DR PATRIC; fig|220668.9.peg.993; -.
DR eggNOG; COG0580; Bacteria.
DR HOGENOM; CLU_020019_9_2_9; -.
DR OMA; MSAGNME; -.
DR PhylomeDB; F9UMX3; -.
DR BioCyc; LPLA220668:G1GW0-1005-MON; -.
DR Proteomes; UP000000432; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015267; F:channel activity; IEA:InterPro.
DR Gene3D; 1.20.1080.10; -; 1.
DR InterPro; IPR023271; Aquaporin-like.
DR InterPro; IPR000425; MIP.
DR Pfam; PF00230; MIP; 1.
DR PRINTS; PR00783; MINTRINSICP.
DR SUPFAM; SSF81338; SSF81338; 1.
PE 3: Inferred from homology;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..238
FT /note="Glycerol uptake facilitator protein 4"
FT /id="PRO_0000441645"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOTIF 62..64
FT /note="NPA 1"
FT /evidence="ECO:0000305|PubMed:23799297"
FT MOTIF 185..187
FT /note="NPA 2"
FT /evidence="ECO:0000305|PubMed:23799297"
SQ SEQUENCE 238 AA; 25367 MW; 513701808D5D62C5 CRC64;
MIHQLLAEFM GTALMIIFGV GVHCSEVLKG TKYRGSGHIF AITTWGFGIT IALFIFGNVC
INPAMVLAQC ILGNLSWSLF IPYSVAEVLG GVVGAVIVWI MYADHFAASA DEISPITIRN
LFSTAPAVRN LPRNFFVEFF DTFIFISGIL AISEVKTPGI VPIGVGLLVW AIGMGLGGPT
GFAMNLARDM GPRIAHAILP IKNKADSDWQ YGIIVPGIAP FVGAACAALF MHGFFGIG
