GLPF_BACSU
ID GLPF_BACSU Reviewed; 274 AA.
AC P18156;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 2.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Glycerol uptake facilitator protein {ECO:0000250|UniProtKB:P0AER0};
GN Name=glpF; OrderedLocusNames=BSU09280;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8436953; DOI=10.1099/00221287-139-2-349;
RA Beijer L., Nilsson R.-P., Holmberg C., Rutberg L.;
RT "The glpP and glpF genes of the glycerol regulon in Bacillus subtilis.";
RL J. Gen. Microbiol. 139:349-359(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9579061; DOI=10.1099/00221287-144-4-859;
RA Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H.,
RA Venema G., Bron S.;
RT "The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus
RT subtilis chromosome contains several dysfunctional genes, the glyB marker,
RT many genes encoding transporter proteins, and the ubiquitous hit gene.";
RL Microbiology 144:859-875(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 94-274.
RX PubMed=2127799; DOI=10.1099/00221287-136-12-2367;
RA Holmberg C., Beijer L., Rutberg B., Rutberg L.;
RT "Glycerol catabolism in Bacillus subtilis: nucleotide sequence of the genes
RT encoding glycerol kinase (glpK) and glycerol-3-phosphate dehydrogenase
RT (glpD).";
RL J. Gen. Microbiol. 136:2367-2375(1990).
CC -!- FUNCTION: Mediates glycerol diffusion across the cytoplasmic membrane
CC via a pore-type mechanism. {ECO:0000250|UniProtKB:P0AER0}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=glycerol(in) = glycerol(out); Xref=Rhea:RHEA:29675,
CC ChEBI:CHEBI:17754; Evidence={ECO:0000250|UniProtKB:P0AER0};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Requires glycerol 3-phosphate and the GlpP product;
CC repressed by glucose.
CC -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC membrane-spanning domains and a pore-forming loop with the signature
CC motif Asn-Pro-Ala (NPA). {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
CC {ECO:0000305}.
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DR EMBL; M99611; AAA22490.1; -; Genomic_DNA.
DR EMBL; Y14079; CAA74428.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12756.1; -; Genomic_DNA.
DR EMBL; M34393; AAA22485.1; ALT_SEQ; Genomic_DNA.
DR PIR; C47700; C47700.
DR RefSeq; NP_388809.1; NC_000964.3.
DR RefSeq; WP_003233386.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; P18156; -.
DR SMR; P18156; -.
DR STRING; 224308.BSU09280; -.
DR TCDB; 1.A.8.2.1; the major intrinsic protein (mip) family.
DR PaxDb; P18156; -.
DR PRIDE; P18156; -.
DR EnsemblBacteria; CAB12756; CAB12756; BSU_09280.
DR GeneID; 939266; -.
DR KEGG; bsu:BSU09280; -.
DR PATRIC; fig|224308.179.peg.1001; -.
DR eggNOG; COG0580; Bacteria.
DR InParanoid; P18156; -.
DR OMA; MTRTGMF; -.
DR PhylomeDB; P18156; -.
DR BioCyc; BSUB:BSU09280-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015254; F:glycerol channel activity; IBA:GO_Central.
DR GO; GO:0015793; P:glycerol transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1080.10; -; 1.
DR InterPro; IPR023271; Aquaporin-like.
DR InterPro; IPR000425; MIP.
DR InterPro; IPR022357; MIP_CS.
DR Pfam; PF00230; MIP; 1.
DR PRINTS; PR00783; MINTRINSICP.
DR SUPFAM; SSF81338; SSF81338; 1.
DR TIGRFAMs; TIGR00861; MIP; 1.
DR PROSITE; PS00221; MIP; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..274
FT /note="Glycerol uptake facilitator protein"
FT /id="PRO_0000064077"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 238..258
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOTIF 64..66
FT /note="NPA 1"
FT /evidence="ECO:0000305"
FT MOTIF 185..187
FT /note="NPA 2"
FT /evidence="ECO:0000305"
SQ SEQUENCE 274 AA; 28735 MW; 8624D0046A63075D CRC64;
MTAFWGEVIG TMLLIIFGAG VCAGVNLKKS LSFQSGWIVV VFGWGLGVAM AAYAVGGISG
AHLNPALTIA LAFVGDFPWK EVPVYIAAQM IGAIIGAVII YLHYLPHWKS TDDPAAKLGV
FSTGPSIPHT FANVLSEVIG TFVLVLGILA IGANQFTEGL NPLIVGFLIV AIGISLGGTT
GYAINPARDL GPRIAHAFLP IPGKGSSNWK YAWVPVVGPI LGGSFGGVFY NAAFKGHITS
SFWIVSVILV VVLLGLYVYT KSHSAKTLSN SKYI
