GLPF_MYCGA
ID GLPF_MYCGA Reviewed; 243 AA.
AC P52280;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 15-AUG-2003, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Probable glycerol uptake facilitator protein {ECO:0000250|UniProtKB:P0AER0};
GN Name=glpF; OrderedLocusNames=MYCGA0140; ORFNames=MGA_0641;
OS Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
OS (Mycoplasmoides gallisepticum).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=710127;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S6;
RA Forsyth M.H., Saoud S., Geary S.J.;
RT "Gene encoding a gycerol uptake protein from Mycoplasma gallisepticum,
RT strain S6.";
RL Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=R(low / passage 15 / clone 2);
RX PubMed=12949158; DOI=10.1099/mic.0.26427-0;
RA Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F.,
RA Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.;
RT "The complete genome sequence of the avian pathogen Mycoplasma
RT gallisepticum strain R(low).";
RL Microbiology 149:2307-2316(2003).
CC -!- FUNCTION: Mediates glycerol diffusion across the cytoplasmic membrane
CC via a pore-type mechanism. {ECO:0000250|UniProtKB:P0AER0}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=glycerol(in) = glycerol(out); Xref=Rhea:RHEA:29675,
CC ChEBI:CHEBI:17754; Evidence={ECO:0000250|UniProtKB:P0AER0};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC membrane-spanning domains and a pore-forming loop with the signature
CC motif Asn-Pro-Ala (NPA). {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
CC {ECO:0000305}.
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DR EMBL; U35010; AAA79047.1; -; Genomic_DNA.
DR EMBL; AE015450; AAP56364.2; -; Genomic_DNA.
DR RefSeq; WP_011113243.1; NC_004829.2.
DR AlphaFoldDB; P52280; -.
DR SMR; P52280; -.
DR TCDB; 1.A.8.2.3; the major intrinsic protein (mip) family.
DR KEGG; mga:MGA_0641; -.
DR PATRIC; fig|233150.7.peg.16; -.
DR HOGENOM; CLU_020019_9_2_14; -.
DR OMA; MTRTGMF; -.
DR OrthoDB; 1744995at2; -.
DR Proteomes; UP000001418; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015267; F:channel activity; IEA:InterPro.
DR Gene3D; 1.20.1080.10; -; 1.
DR InterPro; IPR023271; Aquaporin-like.
DR InterPro; IPR000425; MIP.
DR InterPro; IPR022357; MIP_CS.
DR Pfam; PF00230; MIP; 1.
DR PRINTS; PR00783; MINTRINSICP.
DR SUPFAM; SSF81338; SSF81338; 1.
DR PROSITE; PS00221; MIP; 1.
PE 3: Inferred from homology;
KW Cell membrane; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..243
FT /note="Probable glycerol uptake facilitator protein"
FT /id="PRO_0000064086"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOTIF 72..74
FT /note="NPA 1"
FT /evidence="ECO:0000305"
FT MOTIF 187..189
FT /note="NPA 2"
FT /evidence="ECO:0000305"
FT CONFLICT 28
FT /note="S -> C (in Ref. 1; AAA79047)"
FT /evidence="ECO:0000305"
FT CONFLICT 32
FT /note="K -> E (in Ref. 1; AAA79047)"
FT /evidence="ECO:0000305"
FT CONFLICT 48
FT /note="L -> F (in Ref. 1; AAA79047)"
FT /evidence="ECO:0000305"
FT CONFLICT 70
FT /note="N -> H (in Ref. 1; AAA79047)"
FT /evidence="ECO:0000305"
FT CONFLICT 204..243
FT /note="AFNKKLQNPVSADFRYGLVPLLAPIAAGLIMGGFSLLINQ -> VV (in
FT Ref. 1; AAA79047)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 243 AA; 26194 MW; 43594672BCE4990C CRC64;
MNTTVNILLG ELIGTMVLII LGNGVCASVN YKNMFAKQVG ANWLLIALGW GFAVFCGVVI
SIQVLKGNAN LNPAVTVYFM INQKGANIGL LFAMIAMQLL GAMIAQIILN SLNWKHIIEN
DAEMLKASSC TGPSHRKAWF RNISYEMLGT MILLAGVMAG DYHKLTGVFF VMAIVMSLGS
VTGCAINPAR DFGPRVIYFL TAKAFNKKLQ NPVSADFRYG LVPLLAPIAA GLIMGGFSLL
INQ
