ID   GLPF_MYCGE              Reviewed;         258 AA.
AC   P47279;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=Probable glycerol uptake facilitator protein {ECO:0000250|UniProtKB:P0AER0};
GN   Name=glpF; OrderedLocusNames=MG033;
OS   Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
OS   (Mycoplasmoides genitalium).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=243273;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX   PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA   Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA   Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA   Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA   Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA   Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA   Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT   "The minimal gene complement of Mycoplasma genitalium.";
RL   Science 270:397-403(1995).
CC   -!- FUNCTION: Mediates glycerol diffusion across the cytoplasmic membrane
CC       via a pore-type mechanism. {ECO:0000250|UniProtKB:P0AER0}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glycerol(in) = glycerol(out); Xref=Rhea:RHEA:29675,
CC         ChEBI:CHEBI:17754; Evidence={ECO:0000250|UniProtKB:P0AER0};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC       membrane-spanning domains and a pore-forming loop with the signature
CC       motif Asn-Pro-Ala (NPA). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L43967; AAC71249.1; -; Genomic_DNA.
DR   RefSeq; WP_009885697.1; NZ_AAGX01000003.1.
DR   AlphaFoldDB; P47279; -.
DR   SMR; P47279; -.
DR   STRING; 243273.MG_033; -.
DR   EnsemblBacteria; AAC71249; AAC71249; MG_033.
DR   KEGG; mge:MG_033; -.
DR   eggNOG; COG0580; Bacteria.
DR   HOGENOM; CLU_020019_9_2_14; -.
DR   OMA; MTRTGMF; -.
DR   OrthoDB; 1744995at2; -.
DR   BioCyc; MGEN243273:G1GJ2-33-MON; -.
DR   Proteomes; UP000000807; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0015254; F:glycerol channel activity; IBA:GO_Central.
DR   GO; GO:0015793; P:glycerol transmembrane transport; IBA:GO_Central.
DR   Gene3D; 1.20.1080.10; -; 1.
DR   InterPro; IPR023271; Aquaporin-like.
DR   InterPro; IPR000425; MIP.
DR   InterPro; IPR022357; MIP_CS.
DR   Pfam; PF00230; MIP; 1.
DR   PRINTS; PR00783; MINTRINSICP.
DR   SUPFAM; SSF81338; SSF81338; 1.
DR   PROSITE; PS00221; MIP; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Reference proteome; Repeat; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..258
FT                   /note="Probable glycerol uptake facilitator protein"
FT                   /id="PRO_0000064087"
FT   TRANSMEM        11..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        50..70
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        95..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        152..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        180..200
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        233..253
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOTIF           77..79
FT                   /note="NPA 1"
FT                   /evidence="ECO:0000305"
FT   MOTIF           206..208
FT                   /note="NPA 2"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   258 AA;  27667 MW;  238DBE6ABA4E1438 CRC64;
     MYFQNSTQLG WWFLAELIGT FILIIFGNGA VAQVNLKKMA TSETKAKFLT VALTWGIGVL
     FGVLTANAIF KGSGHLNPAI SLFYAINGSI KSPTALIWPG FVIGILAQFL GAMIAQTTLN
     FLFWKQLSST DPQTVLAMHC TSPSVFNITR NFLTEFIATL ILIGGVVAAS HFLHNNPNSV
     PPGFMGLWLV AGIIIAFGGA TGSAINPARD LGTRIVFQLT PIKNKDANWK YSWIPVIAPL
     SAGLVLSIII GFSPAPVL