ID   GLPF_STRPN              Reviewed;         234 AA.
AC   P0A3Q7; P52281;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Glycerol uptake facilitator protein {ECO:0000250|UniProtKB:P0AER0};
GN   Name=glpF; OrderedLocusNames=SP_2184;
OS   Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=170187;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=P13;
RX   PubMed=7565084; DOI=10.1111/j.1365-2958.1995.tb02294.x;
RA   Saluja S.K., Weiser J.N.;
RT   "The genetic basis of colony opacity in Streptococcus pneumoniae: evidence
RT   for the effect of box elements on the frequency of phenotypic variation.";
RL   Mol. Microbiol. 16:215-227(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-334 / TIGR4;
RX   PubMed=11463916; DOI=10.1126/science.1061217;
RA   Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA   Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA   Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA   Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA   Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA   McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K.,
RA   Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A.,
RA   Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT   "Complete genome sequence of a virulent isolate of Streptococcus
RT   pneumoniae.";
RL   Science 293:498-506(2001).
CC   -!- FUNCTION: Mediates glycerol diffusion across the cytoplasmic membrane
CC       via a pore-type mechanism. {ECO:0000250|UniProtKB:P0AER0}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glycerol(in) = glycerol(out); Xref=Rhea:RHEA:29675,
CC         ChEBI:CHEBI:17754; Evidence={ECO:0000250|UniProtKB:P0AER0};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC       membrane-spanning domains and a pore-forming loop with the signature
CC       motif Asn-Pro-Ala (NPA). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U12567; AAA91618.1; -; Genomic_DNA.
DR   EMBL; AE005672; AAK76235.1; -; Genomic_DNA.
DR   PIR; B95255; B95255.
DR   PIR; S67937; S67937.
DR   RefSeq; WP_000980835.1; NZ_AKVY01000001.1.
DR   AlphaFoldDB; P0A3Q7; -.
DR   SMR; P0A3Q7; -.
DR   STRING; 170187.SP_2184; -.
DR   EnsemblBacteria; AAK76235; AAK76235; SP_2184.
DR   KEGG; spn:SP_2184; -.
DR   eggNOG; COG0580; Bacteria.
DR   OMA; GDLPWAS; -.
DR   PhylomeDB; P0A3Q7; -.
DR   BioCyc; SPNE170187:G1FZB-2282-MON; -.
DR   Proteomes; UP000000585; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015267; F:channel activity; IEA:InterPro.
DR   Gene3D; 1.20.1080.10; -; 1.
DR   InterPro; IPR023271; Aquaporin-like.
DR   InterPro; IPR000425; MIP.
DR   Pfam; PF00230; MIP; 1.
DR   PRINTS; PR00783; MINTRINSICP.
DR   SUPFAM; SSF81338; SSF81338; 1.
DR   TIGRFAMs; TIGR00861; MIP; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Repeat; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..234
FT                   /note="Glycerol uptake facilitator protein"
FT                   /id="PRO_0000064090"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        37..57
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        83..103
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        135..155
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        159..179
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        214..234
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOTIF           65..67
FT                   /note="NPA 1"
FT                   /evidence="ECO:0000305"
FT   MOTIF           186..188
FT                   /note="NPA 2"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        44..45
FT                   /note="GW -> V (in Ref. 1; AAA91618)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        63
FT                   /note="Y -> H (in Ref. 1; AAA91618)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   234 AA;  24371 MW;  221BDBC7E32ECB01 CRC64;
     MMNELFGEFL GTLILILLGN GVVAGVVLPK TKSNSSGWIV ITMGWGIAVA VAVFVSGKLS
     PAYLNPAVTI GVALKGGLPW ASVLPYILAQ FAGAMLGQIL VWLQFKPHYE AEENAGNILA
     TFSTGPAIKD TVSNLISEIL GTFVLVLTIF ALGLYDFQAG IGTFAVGTLI VGIGLSLGGT
     TGYALNPARD LGPRIMHSIL PIPNKGDGDW SYAWIPVVGP VIGAALAVLV FSLF