ID   GLPF_STRR6              Reviewed;         234 AA.
AC   P0A3Q8; P52281;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Glycerol uptake facilitator protein {ECO:0000250|UniProtKB:P0AER0};
GN   Name=glpF; OrderedLocusNames=spr1988;
OS   Streptococcus pneumoniae (strain ATCC BAA-255 / R6).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=171101;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-255 / R6;
RX   PubMed=11544234; DOI=10.1128/jb.183.19.5709-5717.2001;
RA   Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S.,
RA   DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C.,
RA   Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J.,
RA   Lee L.N., Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M.,
RA   McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B.,
RA   Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y.,
RA   Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R.,
RA   Rosteck P.R. Jr., Skatrud P.L., Glass J.I.;
RT   "Genome of the bacterium Streptococcus pneumoniae strain R6.";
RL   J. Bacteriol. 183:5709-5717(2001).
CC   -!- FUNCTION: Mediates glycerol diffusion across the cytoplasmic membrane
CC       via a pore-type mechanism. {ECO:0000250|UniProtKB:P0AER0}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glycerol(in) = glycerol(out); Xref=Rhea:RHEA:29675,
CC         ChEBI:CHEBI:17754; Evidence={ECO:0000250|UniProtKB:P0AER0};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DOMAIN: Aquaporins contain two tandem repeats each containing three
CC       membrane-spanning domains and a pore-forming loop with the signature
CC       motif Asn-Pro-Ala (NPA). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
CC       {ECO:0000305}.
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DR   EMBL; AE007317; AAL00790.1; -; Genomic_DNA.
DR   PIR; A99520; A99520.
DR   RefSeq; NP_359579.1; NC_003098.1.
DR   RefSeq; WP_000980827.1; NC_003098.1.
DR   AlphaFoldDB; P0A3Q8; -.
DR   SMR; P0A3Q8; -.
DR   STRING; 171101.spr1988; -.
DR   EnsemblBacteria; AAL00790; AAL00790; spr1988.
DR   GeneID; 60232961; -.
DR   KEGG; spr:spr1988; -.
DR   PATRIC; fig|171101.6.peg.2152; -.
DR   eggNOG; COG0580; Bacteria.
DR   HOGENOM; CLU_020019_9_2_9; -.
DR   OMA; GDLPWAS; -.
DR   Proteomes; UP000000586; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0015254; F:glycerol channel activity; IBA:GO_Central.
DR   GO; GO:0015793; P:glycerol transmembrane transport; IBA:GO_Central.
DR   Gene3D; 1.20.1080.10; -; 1.
DR   InterPro; IPR023271; Aquaporin-like.
DR   InterPro; IPR000425; MIP.
DR   InterPro; IPR022357; MIP_CS.
DR   Pfam; PF00230; MIP; 1.
DR   PRINTS; PR00783; MINTRINSICP.
DR   SUPFAM; SSF81338; SSF81338; 1.
DR   TIGRFAMs; TIGR00861; MIP; 1.
DR   PROSITE; PS00221; MIP; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Reference proteome; Repeat; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..234
FT                   /note="Glycerol uptake facilitator protein"
FT                   /id="PRO_0000064091"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        37..57
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        83..103
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        135..155
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        159..179
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        214..234
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOTIF           65..67
FT                   /note="NPA 1"
FT                   /evidence="ECO:0000305"
FT   MOTIF           186..188
FT                   /note="NPA 2"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   234 AA;  24345 MW;  497670A3A6336065 CRC64;
     MMNELFGEFL GTLILILLGN GVVAGVVLPK TKSNSSGWIV ITMGWGIAVA VAVFVSGKLS
     PAHLNPAVTI GVALKGGLPW ASVLPYILAQ FAGAMLGQIL VWLQFKPHYE AEENAGNILA
     TFSTGPAIKD TVSNLISEIL GTFVLVLTIF ALGLYDFQAG IGTFAVGTLI VGIGLSLGGT
     TGYALNPARD LGPRIMHSIL PIPNKGDGDW SYAWIPVVGP VIGAALAVLV FSLF