ID   AMHM_ALKPO              Reviewed;         167 AA.
AC   D3FSJ2; O50575;
DT   26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT   23-MAR-2010, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Ammonium/H(+) antiporter subunit AmhM;
DE   AltName: Full=Accessory protein for AmhT;
GN   Name=amhM; OrderedLocusNames=BpOF4_09525;
OS   Alkalihalophilus pseudofirmus (strain ATCC BAA-2126 / JCM 17055 / OF4)
OS   (Bacillus pseudofirmus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalophilus.
OX   NCBI_TaxID=398511;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC BAA-2126 / JCM 17055 / OF4;
RX   PubMed=12923086; DOI=10.1128/jb.185.17.5133-5147.2003;
RA   Wei Y., Southworth T.W., Kloster H., Ito M., Guffanti A.A., Moir A.,
RA   Krulwich T.A.;
RT   "Mutational loss of a K+ and NH4+ transporter affects the growth and
RT   endospore formation of alkaliphilic Bacillus pseudofirmus OF4.";
RL   J. Bacteriol. 185:5133-5147(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-2126 / JCM 17055 / OF4;
RX   PubMed=21951522; DOI=10.1111/j.1462-2920.2011.02591.x;
RA   Janto B., Ahmed A., Ito M., Liu J., Hicks D.B., Pagni S., Fackelmayer O.J.,
RA   Smith T.A., Earl J., Elbourne L.D., Hassan K., Paulsen I.T., Kolsto A.B.,
RA   Tourasse N.J., Ehrlich G.D., Boissy R., Ivey D.M., Li G., Xue Y., Ma Y.,
RA   Hu F.Z., Krulwich T.A.;
RT   "Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that
RT   support the ability to grow in an external pH range from 7.5 to 11.4.";
RL   Environ. Microbiol. 13:3289-3309(2011).
RN   [3]
RP   FUNCTION, AND SUBUNIT.
RC   STRAIN=ATCC BAA-2126 / JCM 17055 / OF4;
RX   PubMed=17679694; DOI=10.1073/pnas.0703709104;
RA   Fujisawa M., Ito M., Krulwich T.A.;
RT   "Three two-component transporters with channel-like properties have
RT   monovalent cation/proton antiport activity.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:13289-13294(2007).
CC   -!- FUNCTION: Modulates the activity of the ammonium/proton antiporter
CC       AmhT. {ECO:0000269|PubMed:12923086, ECO:0000269|PubMed:17679694}.
CC   -!- SUBUNIT: Interacts with AmhT. {ECO:0000305|PubMed:17679694}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
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DR   EMBL; U89914; AAB87746.1; -; Genomic_DNA.
DR   EMBL; CP001878; ADC49960.1; -; Genomic_DNA.
DR   PIR; T52551; T52551.
DR   RefSeq; WP_012957326.1; NC_013791.2.
DR   AlphaFoldDB; D3FSJ2; -.
DR   SMR; D3FSJ2; -.
DR   STRING; 398511.BpOF4_09525; -.
DR   TCDB; 2.A.37.5.1; the monovalent cation:proton antiporter-2 (cpa2) family.
DR   EnsemblBacteria; ADC49960; ADC49960; BpOF4_09525.
DR   KEGG; bpf:BpOF4_09525; -.
DR   eggNOG; COG0490; Bacteria.
DR   HOGENOM; CLU_116143_0_0_9; -.
DR   OMA; EGAYFQP; -.
DR   OrthoDB; 941006at2; -.
DR   Proteomes; UP000001544; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008324; F:cation transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006813; P:potassium ion transport; IEA:InterPro.
DR   Gene3D; 3.30.70.1450; -; 1.
DR   InterPro; IPR026278; K(+)/H(+)_antiporter_KhtT.
DR   InterPro; IPR006037; RCK_C.
DR   InterPro; IPR036721; RCK_C_sf.
DR   Pfam; PF02080; TrkA_C; 1.
DR   PIRSF; PIRSF005028; KhtT; 1.
DR   SUPFAM; SSF116726; SSF116726; 1.
DR   PROSITE; PS51202; RCK_C; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Reference proteome.
FT   CHAIN           1..167
FT                   /note="Ammonium/H(+) antiporter subunit AmhM"
FT                   /id="PRO_0000422713"
FT   DOMAIN          79..163
FT                   /note="RCK C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00544"
SQ   SEQUENCE   167 AA;  18525 MW;  9292713CC1E16A19 CRC64;
     MKITSGDLPG VGKKISFITS EGSMVVLVIH HTGKREMYFF DDADDDEVSF SLTLSAEETK
     QMGAQLLGAI LNPADTDKID RIKLIRKQVV VEWIDITKHS PIISKSIAQI EKMKPKGISI
     VGVFKNDEMM VDPEPTLVLE KGDTLMAVGK RDAIQKFEEL CACKENN