AMHT_ALKPO
ID AMHT_ALKPO Reviewed; 390 AA.
AC D3FSJ3; O50576;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 23-MAR-2010, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Ammonium/H(+) antiporter subunit AmhT;
GN Name=amhT; OrderedLocusNames=BpOF4_09530;
OS Alkalihalophilus pseudofirmus (strain ATCC BAA-2126 / JCM 17055 / OF4)
OS (Bacillus pseudofirmus).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalophilus.
OX NCBI_TaxID=398511;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC BAA-2126 / JCM 17055 / OF4;
RX PubMed=12923086; DOI=10.1128/jb.185.17.5133-5147.2003;
RA Wei Y., Southworth T.W., Kloster H., Ito M., Guffanti A.A., Moir A.,
RA Krulwich T.A.;
RT "Mutational loss of a K+ and NH4+ transporter affects the growth and
RT endospore formation of alkaliphilic Bacillus pseudofirmus OF4.";
RL J. Bacteriol. 185:5133-5147(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-2126 / JCM 17055 / OF4;
RX PubMed=21951522; DOI=10.1111/j.1462-2920.2011.02591.x;
RA Janto B., Ahmed A., Ito M., Liu J., Hicks D.B., Pagni S., Fackelmayer O.J.,
RA Smith T.A., Earl J., Elbourne L.D., Hassan K., Paulsen I.T., Kolsto A.B.,
RA Tourasse N.J., Ehrlich G.D., Boissy R., Ivey D.M., Li G., Xue Y., Ma Y.,
RA Hu F.Z., Krulwich T.A.;
RT "Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that
RT support the ability to grow in an external pH range from 7.5 to 11.4.";
RL Environ. Microbiol. 13:3289-3309(2011).
RN [3]
RP FUNCTION AS AN ANTIPORTER, ACTIVITY REGULATION, AND SUBUNIT.
RC STRAIN=ATCC BAA-2126 / JCM 17055 / OF4;
RX PubMed=17679694; DOI=10.1073/pnas.0703709104;
RA Fujisawa M., Ito M., Krulwich T.A.;
RT "Three two-component transporters with channel-like properties have
RT monovalent cation/proton antiport activity.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:13289-13294(2007).
CC -!- FUNCTION: Ammonium/proton antiporter that mediates the efflux of
CC ammonium ions. Can also transport potassium or rubidium, but not sodium
CC or lithium. {ECO:0000269|PubMed:12923086, ECO:0000269|PubMed:17679694}.
CC -!- ACTIVITY REGULATION: AmhT alone exhibits antiport activity, but
CC interaction with AmhM confers different properties, such as higher KM
CC for potassium. {ECO:0000269|PubMed:17679694}.
CC -!- SUBUNIT: Interacts with AmhM. {ECO:0000305|PubMed:17679694}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Deletion affects endospore formation and
CC germination. {ECO:0000269|PubMed:12923086}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 2 (CPA2)
CC transporter (TC 2.A.37) family. {ECO:0000305}.
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DR EMBL; U89914; AAB87747.2; -; Genomic_DNA.
DR EMBL; CP001878; ADC49961.1; -; Genomic_DNA.
DR PIR; T52552; T52552.
DR RefSeq; WP_012957327.1; NC_013791.2.
DR AlphaFoldDB; D3FSJ3; -.
DR SMR; D3FSJ3; -.
DR STRING; 398511.BpOF4_09530; -.
DR TCDB; 2.A.37.5.1; the monovalent cation:proton antiporter-2 (cpa2) family.
DR EnsemblBacteria; ADC49961; ADC49961; BpOF4_09530.
DR KEGG; bpf:BpOF4_09530; -.
DR eggNOG; COG0475; Bacteria.
DR HOGENOM; CLU_005126_4_2_9; -.
DR OMA; KWSAKER; -.
DR OrthoDB; 941006at2; -.
DR Proteomes; UP000001544; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015299; F:solute:proton antiporter activity; IEA:InterPro.
DR GO; GO:0006812; P:cation transport; IEA:InterPro.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR Pfam; PF00999; Na_H_Exchanger; 1.
PE 1: Evidence at protein level;
KW Ammonia transport; Antiport; Cell membrane; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..390
FT /note="Ammonium/H(+) antiporter subunit AmhT"
FT /id="PRO_0000422714"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 266..286
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 390 AA; 41791 MW; 2669D69C32C98F80 CRC64;
MVIPELFSAG LILLLLFITG FVGMKMKIPD VVIFILLGIA VGGLLSGSHL LHFAGEVGIV
LLFFMLGMEF PLKQLMSIAK KVLRAGILDV ALSFGVTMAI CMMMGLDVIT SLIIGGVAYA
TSSSITAKML ESSKRMANPE SEFMLGLLIF EDLVAPILVA VLVGLTAGMA LTAGSMSLLV
VKVVALVAGA VILGVFLFRK LGSFFDRHMK HDLFILFVIG LALMYGGLAL YLDLSEVLGA
FLAGIMLAEV KRTHELELMV VRFRDLLLPL FFLYFGTTIS FSEGIPMIPL LILVLVWSVI
AKVIVGVLGG RWYGLTKKVS LRAGLSLTQR GEFSIIIASL AAGSIKAFSS VFILASAMIG
ILLFQFAPSI ANKFYGKKAK TSVKQHVGSA
