ID   AMHX_BACSU              Reviewed;         383 AA.
AC   P54983;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 3.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=Amidohydrolase AmhX;
DE            EC=3.5.1.-;
DE   AltName: Full=Aminoacylase;
GN   Name=amhX; OrderedLocusNames=BSU03010;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / JH642;
RX   PubMed=8768514; DOI=10.1111/j.1574-6968.1996.tb08374.x;
RA   Kempf B., Bremer E.;
RT   "A novel amidohydrolase gene from Bacillus subtilis cloning: DNA-sequence
RT   analysis and map position of amhX.";
RL   FEMS Microbiol. Lett. 141:129-137(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA   Yamane K., Kumano M., Kurita K.;
RT   "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT   determination of the sequence of a 146 kb segment and identification of 113
RT   genes.";
RL   Microbiology 142:3047-3056(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   SEQUENCE REVISION TO C-TERMINUS.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 335-383.
RC   STRAIN=168 / JH642;
RX   PubMed=7622480; DOI=10.1074/jbc.270.28.16701;
RA   Kempf B., Bremer E.;
RT   "OpuA, an osmotically regulated binding protein-dependent transport system
RT   for the osmoprotectant glycine betaine in Bacillus subtilis.";
RL   J. Biol. Chem. 270:16701-16713(1995).
CC   -!- SIMILARITY: Belongs to the peptidase M20 family. {ECO:0000305}.
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DR   EMBL; U47860; AAC44168.1; -; Genomic_DNA.
DR   EMBL; D50453; BAA08935.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12095.2; -; Genomic_DNA.
DR   EMBL; U17292; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; B69585; B69585.
DR   RefSeq; NP_388183.2; NC_000964.3.
DR   RefSeq; WP_003246319.1; NZ_JNCM01000030.1.
DR   AlphaFoldDB; P54983; -.
DR   SMR; P54983; -.
DR   STRING; 224308.BSU03010; -.
DR   MEROPS; M20.A26; -.
DR   PaxDb; P54983; -.
DR   PRIDE; P54983; -.
DR   EnsemblBacteria; CAB12095; CAB12095; BSU_03010.
DR   GeneID; 938351; -.
DR   KEGG; bsu:BSU03010; -.
DR   PATRIC; fig|224308.179.peg.313; -.
DR   eggNOG; COG1473; Bacteria.
DR   InParanoid; P54983; -.
DR   OMA; DSHMTMA; -.
DR   PhylomeDB; P54983; -.
DR   BioCyc; BSUB:BSU03010-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016787; F:hydrolase activity; IBA:GO_Central.
DR   CDD; cd08018; M20_Acy1_amhX-like; 1.
DR   InterPro; IPR037484; AmhX-like.
DR   InterPro; IPR017439; Amidohydrolase.
DR   InterPro; IPR036264; Bact_exopeptidase_dim_dom.
DR   InterPro; IPR002933; Peptidase_M20.
DR   InterPro; IPR011650; Peptidase_M20_dimer.
DR   PANTHER; PTHR11014; PTHR11014; 1.
DR   Pfam; PF07687; M20_dimer; 1.
DR   Pfam; PF01546; Peptidase_M20; 1.
DR   PIRSF; PIRSF005962; Pept_M20D_amidohydro; 1.
DR   SUPFAM; SSF55031; SSF55031; 1.
DR   TIGRFAMs; TIGR01891; amidohydrolases; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..383
FT                   /note="Amidohydrolase AmhX"
FT                   /id="PRO_0000061951"
FT   CONFLICT        161
FT                   /note="R -> A (in Ref. 1; AAC44168)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        367..383
FT                   /note="VLKTFQKAESLAAANAS -> CSKHFRRRNHLLPLTLLNAKKAA (in
FT                   Ref. 2; BAA08935)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   383 AA;  41591 MW;  162496395CF67A71 CRC64;
     MQKLSEEMKQ TIMDIFEHLH ANPEVSWKEY ETTSFLKQKL EDLGCRTRTF SDCTGVVGEI
     GSGSPVVAVR ADIDALWQEV NGTFRANHSC GHDSHMTMAL GTLMLLKKQP ELPKGTIRFI
     FQPAEEKGGG ALKMIEEGVL DDIDYLYGVH VRPIQETQNG RCAPSILHGS SQHIEGTIIG
     EEAHGARPHL GKNSIEIAAF LVHKLGLIHI DPQIPHTVKM TKLQAGGESS NIIPGKASFS
     LDLRAQTNEA MEALIAETER ACEAAAAAFG AKIELHKEHS LPAATQNKEA EAIMAEAITE
     IIGAERLDDP LVTTGGEDFH FYAVKVPNLK TTMLGLGCGL QPGLHHPHMT FDRNAMFTGI
     HILANAVLKT FQKAESLAAA NAS