ID   AMI3_MYCTO              Reviewed;         473 AA.
AC   P9WQ94; L0TAY0; P63494; Q10811;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Putative amidase AmiC;
DE            EC=3.5.1.4;
GN   Name=amiC; OrderedLocusNames=MT2956;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a monocarboxylic acid amide + H2O = a monocarboxylate +
CC         NH4(+); Xref=Rhea:RHEA:12020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:35757, ChEBI:CHEBI:83628; EC=3.5.1.4;
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
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DR   EMBL; AE000516; AAK47281.1; -; Genomic_DNA.
DR   PIR; C70925; C70925.
DR   RefSeq; WP_003414672.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WQ94; -.
DR   SMR; P9WQ94; -.
DR   EnsemblBacteria; AAK47281; AAK47281; MT2956.
DR   KEGG; mtc:MT2956; -.
DR   PATRIC; fig|83331.31.peg.3193; -.
DR   HOGENOM; CLU_009600_0_4_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004040; F:amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0043864; F:indoleacetamide hydrolase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR000120; Amidase.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR11895; PTHR11895; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   3: Inferred from homology;
KW   Hydrolase.
FT   CHAIN           1..473
FT                   /note="Putative amidase AmiC"
FT                   /id="PRO_0000426815"
FT   ACT_SITE        82
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        157
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        181
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   473 AA;  50916 MW;  E01EF4572EF8D47C CRC64;
     MSRVHAFVDD ALGDLDAVAL ADAIRSGRVG RADVVEAAIA RAEAVNPALN ALAYAAFDVA
     RDAAAMGTGQ EAFFSGVPTF IKDNVDVAGQ PSMHGTDAWE PYAAVADSEI TRVVLGTGLV
     SLGKTQLSEF GFSAVAEHPR LGPVRNPWNT DYTAGASSSG SGALVAAGVV PIAHANDGGG
     SIRIPAACNG LVGLKPSRGR LPLEPEYRRL PVGIVANGVL TRTVRDTAAF YREAERLWRN
     HQLPPVGDVT SPVKQRLRIA VVTRSVLREA SPEVRQLTLK LAGLLEELGH RVEHVDHPPA
     PASFVDDFVL YWGFLALAQV RSGRRTFGRT FDPTRLDELT LGLARHTGRN LHRLPLAIMR
     LRMLRRRSVR FFGTYDVLLT PTVAEATPQV GYLAPTDYQT VLDRLSSWVV FTPVQNVTGV
     PAISLPLAQS ADGMPVGMML SADTGREALL LELAYELEEA RPWARIHAPN IAE