ID   AMI3_MYCTU              Reviewed;         473 AA.
AC   P9WQ95; L0TAY0; P63494; Q10811;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 37.
DE   RecName: Full=Putative amidase AmiC;
DE            EC=3.5.1.4;
GN   Name=amiC; OrderedLocusNames=Rv2888c; ORFNames=MTCY274.19c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a monocarboxylic acid amide + H2O = a monocarboxylate +
CC         NH4(+); Xref=Rhea:RHEA:12020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:35757, ChEBI:CHEBI:83628; EC=3.5.1.4;
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP45690.1; -; Genomic_DNA.
DR   PIR; C70925; C70925.
DR   RefSeq; NP_217404.1; NC_000962.3.
DR   RefSeq; WP_003414672.1; NZ_NVQJ01000006.1.
DR   AlphaFoldDB; P9WQ95; -.
DR   SMR; P9WQ95; -.
DR   STRING; 83332.Rv2888c; -.
DR   PaxDb; P9WQ95; -.
DR   DNASU; 887401; -.
DR   GeneID; 887401; -.
DR   KEGG; mtu:Rv2888c; -.
DR   TubercuList; Rv2888c; -.
DR   eggNOG; COG0154; Bacteria.
DR   OMA; WNTDHTA; -.
DR   PhylomeDB; P9WQ95; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0004040; F:amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0043864; F:indoleacetamide hydrolase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR000120; Amidase.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR11895; PTHR11895; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..473
FT                   /note="Putative amidase AmiC"
FT                   /id="PRO_0000105258"
FT   ACT_SITE        82
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        157
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        181
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   473 AA;  50916 MW;  E01EF4572EF8D47C CRC64;
     MSRVHAFVDD ALGDLDAVAL ADAIRSGRVG RADVVEAAIA RAEAVNPALN ALAYAAFDVA
     RDAAAMGTGQ EAFFSGVPTF IKDNVDVAGQ PSMHGTDAWE PYAAVADSEI TRVVLGTGLV
     SLGKTQLSEF GFSAVAEHPR LGPVRNPWNT DYTAGASSSG SGALVAAGVV PIAHANDGGG
     SIRIPAACNG LVGLKPSRGR LPLEPEYRRL PVGIVANGVL TRTVRDTAAF YREAERLWRN
     HQLPPVGDVT SPVKQRLRIA VVTRSVLREA SPEVRQLTLK LAGLLEELGH RVEHVDHPPA
     PASFVDDFVL YWGFLALAQV RSGRRTFGRT FDPTRLDELT LGLARHTGRN LHRLPLAIMR
     LRMLRRRSVR FFGTYDVLLT PTVAEATPQV GYLAPTDYQT VLDRLSSWVV FTPVQNVTGV
     PAISLPLAQS ADGMPVGMML SADTGREALL LELAYELEEA RPWARIHAPN IAE