AMI4G_ARATH
ID AMI4G_ARATH Reviewed; 512 AA.
AC A0A1P8B760; Q9SW47;
DT 20-DEC-2017, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2017, sequence version 1.
DT 25-MAY-2022, entry version 29.
DE RecName: Full=Probable amidase At4g34880 {ECO:0000305};
DE EC=3.5.1.4 {ECO:0000250|UniProtKB:Q9FR37};
GN OrderedLocusNames=At4g34880 {ECO:0000312|EMBL:ANM67425.1};
GN ORFNames=F11I11.120 {ECO:0000312|EMBL:ANM67425.1},
GN T11I11.120 {ECO:0000312|EMBL:CAB45449.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=23844031; DOI=10.1371/journal.pone.0067562;
RA Wu X., Huang R., Liu Z., Zhang G.;
RT "Functional characterization of cis-elements conferring vascular vein
RT expression of At4g34880 amidase family protein gene in Arabidopsis.";
RL PLoS ONE 8:E67562-E67562(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a monocarboxylic acid amide + H2O = a monocarboxylate +
CC NH4(+); Xref=Rhea:RHEA:12020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:35757, ChEBI:CHEBI:83628; EC=3.5.1.4;
CC Evidence={ECO:0000250|UniProtKB:Q9FR37};
CC -!- TISSUE SPECIFICITY: Expressed in vasculature of roots, cotyledons,
CC leaves and sepals. {ECO:0000269|PubMed:23844031}.
CC -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AEE86432.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB45449.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80205.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL079347; CAB45449.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161586; CAB80205.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86432.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; ANM67425.1; -; Genomic_DNA.
DR PIR; T10234; T10234.
DR RefSeq; NP_001329257.1; NM_001342309.1.
DR RefSeq; NP_195214.1; NM_119654.2.
DR AlphaFoldDB; A0A1P8B760; -.
DR SMR; A0A1P8B760; -.
DR STRING; 3702.AT4G34880.1; -.
DR ProteomicsDB; 244987; -.
DR EnsemblPlants; AT4G34880.2; AT4G34880.2; AT4G34880.
DR GeneID; 829640; -.
DR Gramene; AT4G34880.2; AT4G34880.2; AT4G34880.
DR KEGG; ath:AT4G34880; -.
DR Araport; AT4G34880; -.
DR TAIR; locus:2116890; AT4G34880.
DR eggNOG; KOG1211; Eukaryota.
DR OMA; DMRSNNY; -.
DR OrthoDB; 1327093at2759; -.
DR PRO; PR:A0A1P8B760; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; A0A1P8B760; baseline and differential.
DR GO; GO:0004040; F:amidase activity; IEA:UniProtKB-EC.
DR GO; GO:0043864; F:indoleacetamide hydrolase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.90.1300.10; -; 1.
DR InterPro; IPR023631; Amidase_dom.
DR InterPro; IPR036928; AS_sf.
DR Pfam; PF01425; Amidase; 1.
DR SUPFAM; SSF75304; SSF75304; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Reference proteome.
FT CHAIN 1..512
FT /note="Probable amidase At4g34880"
FT /id="PRO_5010213546"
FT ACT_SITE 117
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q936X2"
FT ACT_SITE 198
FT /note="Charge relay system"
FT /evidence="ECO:0000250|UniProtKB:Q936X2"
FT ACT_SITE 222
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000250|UniProtKB:Q936X2"
SQ SEQUENCE 512 AA; 54917 MW; FA34C39DF66B671E CRC64;
MVALRFFFFS SLLILLILSQ ALIMSVGSAS QIRLSSTFSI QEATIEDIRV AFNEKRLTSK
QLVELYLEAI SKLNPILHAV IETNPDALIQ AEIADRERDL KNTTKLPILH GVPVLLKDSI
STKDKLNTTA GSFALLGSVV ARDAGVVKRL RESGAVILGK ASLSEWAHFR SFSIPDGWSA
RGLQGKNPYV LSANPSGSSS GSAISVTANL VAVSLGTETD GSILSPASQN SVVGIKPSVG
LTSRAGVVPI SLRQDSIGPI CRTVSDAVHL LDAIVGYDPL DEATKTASEF IPEGGYKQFL
TTSGLKGKRL GIVMKHSSLL DHHIKTLRRE GAIVINNLTI PNIEVIVGGT DSGEEIALLA
EFKMSLNAYL KELVKSPVRS LADVIAYNEE FAEQEKVKEW GQEVFLTAEA TSGMGEKEKT
ALQKMKELSR NGIEKLIEEN KLDAIVTLGS DLSSVLAIGG YPGINVPAGY DSGGVPYGIS
FGGLRFSEPK LIEIAFAFEQ ATLIRKPPKF IA
