AMIA_STRPN
ID AMIA_STRPN Reviewed; 659 AA.
AC P18791; P18792;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 26-SEP-2001, sequence version 3.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Oligopeptide-binding protein AmiA;
DE Flags: Precursor;
GN Name=amiA; OrderedLocusNames=SP_1891;
OS Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=170187;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=R6 / R800;
RX PubMed=2352474; DOI=10.1111/j.1365-2958.1990.tb00632.x;
RA Alloing G., Trombe M.C., Claverys J.-P.;
RT "The ami locus of the Gram-positive bacterium Streptococcus pneumoniae is
RT similar to binding protein-dependent transport operons of Gram-negative
RT bacteria.";
RL Mol. Microbiol. 4:633-644(1990).
RN [2]
RP SEQUENCE REVISION.
RA Claverys J.-P.;
RL Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-334 / TIGR4;
RX PubMed=11463916; DOI=10.1126/science.1061217;
RA Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K.,
RA Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A.,
RA Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT "Complete genome sequence of a virulent isolate of Streptococcus
RT pneumoniae.";
RL Science 293:498-506(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
RC STRAIN=R6 / R800;
RX PubMed=2684766; DOI=10.1016/0378-1119(89)90287-4;
RA Martin B., Alloing G., Boucraut C., Claverys J.-P.;
RT "The difficulty of cloning Streptococcus pneumoniae mal and ami loci in
RT Escherichia coli: toxicity of malX and amiA gene products.";
RL Gene 80:227-238(1989).
RN [5]
RP PARTIAL PROTEIN SEQUENCE.
RX PubMed=3208757; DOI=10.1002/j.1460-2075.1988.tb03284.x;
RA Gilson E., Alloing G., Schmidt T., Claverys J.-P., Dudler R., Hofnung M.;
RT "Evidence for high affinity binding-protein dependent transport systems in
RT Gram-positive bacteria and in Mycoplasma.";
RL EMBO J. 7:3971-3974(1988).
CC -!- FUNCTION: Part of the binding-protein-dependent transport system for
CC oligopeptides; probably an oligopeptide binding protein.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor.
CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 5 family.
CC {ECO:0000305}.
CC -!- CAUTION: The revised sequence of AmiA now includes, in the C-terminal
CC section, the sequence of an ORF which was previously known as AmiB.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X17337; CAA35213.1; -; Genomic_DNA.
DR EMBL; AE005672; AAK75962.1; -; Genomic_DNA.
DR PIR; A95221; A95221.
DR PIR; S11148; S11148.
DR PIR; S11149; S11149.
DR RefSeq; WP_000742235.1; NZ_AKVY01000001.1.
DR AlphaFoldDB; P18791; -.
DR SMR; P18791; -.
DR STRING; 170187.SP_1891; -.
DR PRIDE; P18791; -.
DR EnsemblBacteria; AAK75962; AAK75962; SP_1891.
DR KEGG; spn:SP_1891; -.
DR eggNOG; COG4166; Bacteria.
DR OMA; TWTPDYA; -.
DR PhylomeDB; P18791; -.
DR BioCyc; SPNE170187:G1FZB-1921-MON; -.
DR Proteomes; UP000000585; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProt.
DR GO; GO:0015833; P:peptide transport; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR InterPro; IPR030678; Peptide/Ni-bd.
DR InterPro; IPR039424; SBP_5.
DR InterPro; IPR023765; SBP_5_CS.
DR InterPro; IPR000914; SBP_5_dom.
DR PANTHER; PTHR30290; PTHR30290; 1.
DR Pfam; PF00496; SBP_bac_5; 1.
DR PIRSF; PIRSF002741; MppA; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
DR PROSITE; PS01040; SBP_BACTERIAL_5; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Direct protein sequencing; Lipoprotein; Membrane; Palmitate;
KW Peptide transport; Protein transport; Signal; Transport.
FT SIGNAL 1..22
FT /evidence="ECO:0000305"
FT CHAIN 23..659
FT /note="Oligopeptide-binding protein AmiA"
FT /id="PRO_0000031787"
FT LIPID 23
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000305"
FT LIPID 23
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000305"
FT CONFLICT 54
FT /note="S -> R (in Ref. 1; CAA35213)"
FT /evidence="ECO:0000305"
FT CONFLICT 421
FT /note="A -> R (in Ref. 1; CAA35213)"
FT /evidence="ECO:0000305"
FT CONFLICT 557
FT /note="R -> K (in Ref. 1; CAA35213)"
FT /evidence="ECO:0000305"
FT CONFLICT 562
FT /note="L -> F (in Ref. 1; CAA35213)"
FT /evidence="ECO:0000305"
FT CONFLICT 622
FT /note="S -> L (in Ref. 1; CAA35213)"
FT /evidence="ECO:0000305"
FT CONFLICT 626
FT /note="V -> A (in Ref. 1; CAA35213)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 659 AA; 72465 MW; C2ED06A1DB8A7B81 CRC64;
MKKNRVFATA GLVLLAAGVL AACSSSKSSD SSAPKAYGYV YTADPETLDY LISSKNSTTV
VTSNGIDGLF TNDNYGNLAP AVAEDWEVSK DGLTYTYKIR KGVKWFTSDG EEYAEVTAKD
FVNGLKHAAD KKSEAMYLAE NSVKGLADYL SGTSTDFSTV GVKAVDDYTL QYTLNQPEPF
WNSKLTYSIF WPLNEEFETS KGSDFAKPTD PTSLLYNGPF LLKGLTAKSS VEFVKNEQYW
DKENVHLDTI NLAYYDGSDQ ESLERNFTSG AYSYARLYPT SSNYSKVAEE YKDNIYYTQS
GSGIAGLGVN IDRQSYNYTS KTTDSEKVAT KKALLNKDFR QALNFALDRS AYSAQINGKD
GAALAVRNLF VKPDFVSAGE KTFGDLVAAQ LPAYGDEWKG VNLADGQDGL FNADKAKAEF
AKAKKALEAD GVQFPIHLDV PVDQASKNYI SRIQSFKQSV ETVLGVENVV VDIQQMTSDE
FLNITYYAAN ASSEDWDVSG GVSWGPDYQD PSTYLDILKT TSSETTKTYL GFDNPNSPSV
VQVGLKEYDK LVDEAARETS DLNVRYEKYA AAQAWLTDSS LFIPAMASSG AAPVLSRIVP
FTGASAQTGS KGSDVYFKYL KSQDKVVTKE EYEKAREKWL KEKAESNEKA QKELASHVK
