GLPO_STRPN
ID GLPO_STRPN Reviewed; 608 AA.
AC P35596; O87017;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 26-SEP-2001, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Alpha-glycerophosphate oxidase;
DE EC=1.1.3.21;
DE AltName: Full=Exported protein 6;
DE AltName: Full=Glycerol-3-phosphate oxidase;
GN Name=glpO; Synonyms=exp6; OrderedLocusNames=SP_2185;
OS Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=170187;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 27336;
RX PubMed=9726992; DOI=10.1074/jbc.273.37.23812;
RA Parsonage D., Luba J., Mallett T.C., Claiborne A.;
RT "The soluble alpha-glycerophosphate oxidase from Enterococcus
RT casseliflavus. Sequence homology with the membrane-associated dehydrogenase
RT and kinetic analysis of the recombinant enzyme.";
RL J. Biol. Chem. 273:23812-23822(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-334 / TIGR4;
RX PubMed=11463916; DOI=10.1126/science.1061217;
RA Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K.,
RA Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A.,
RA Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT "Complete genome sequence of a virulent isolate of Streptococcus
RT pneumoniae.";
RL Science 293:498-506(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 271-408.
RC STRAIN=R6x;
RX PubMed=7934910; DOI=10.1111/j.1365-2958.1993.tb01233.x;
RA Pearce B.J., Yin Y.B., Masure H.R.;
RT "Genetic identification of exported proteins in Streptococcus pneumoniae.";
RL Mol. Microbiol. 9:1037-1050(1993).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=O2 + sn-glycerol 3-phosphate = dihydroxyacetone phosphate +
CC H2O2; Xref=Rhea:RHEA:18369, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240,
CC ChEBI:CHEBI:57597, ChEBI:CHEBI:57642; EC=1.1.3.21;
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC -!- PATHWAY: Membrane lipid metabolism; glycerophospholipid metabolism.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC -!- SIMILARITY: Belongs to the FAD-dependent glycerol-3-phosphate
CC dehydrogenase family. {ECO:0000305}.
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DR EMBL; U94770; AAC34740.1; -; Genomic_DNA.
DR EMBL; AE005672; AAK76236.1; -; Genomic_DNA.
DR EMBL; L20560; AAA26882.1; -; Genomic_DNA.
DR PIR; C95255; C95255.
DR RefSeq; WP_000395957.1; NZ_AKVY01000001.1.
DR AlphaFoldDB; P35596; -.
DR SMR; P35596; -.
DR STRING; 170187.SP_2185; -.
DR EnsemblBacteria; AAK76236; AAK76236; SP_2185.
DR KEGG; spn:SP_2185; -.
DR eggNOG; COG0578; Bacteria.
DR OMA; CIVNAAG; -.
DR PhylomeDB; P35596; -.
DR BioCyc; SPNE170187:G1FZB-2283-MON; -.
DR UniPathway; UPA00940; -.
DR Proteomes; UP000000585; Chromosome.
DR GO; GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004368; F:glycerol-3-phosphate dehydrogenase (quinone) activity; IEA:InterPro.
DR GO; GO:0004369; F:glycerol-3-phosphate oxidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006072; P:glycerol-3-phosphate metabolic process; IEA:InterPro.
DR GO; GO:0006650; P:glycerophospholipid metabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.8.870; -; 1.
DR Gene3D; 3.50.50.60; -; 2.
DR InterPro; IPR031656; DAO_C.
DR InterPro; IPR038299; DAO_C_sf.
DR InterPro; IPR006076; FAD-dep_OxRdtase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR000447; G3P_DH_FAD-dep.
DR PANTHER; PTHR11985; PTHR11985; 1.
DR Pfam; PF01266; DAO; 1.
DR Pfam; PF16901; DAO_C; 1.
DR PRINTS; PR01001; FADG3PDH.
DR SUPFAM; SSF51905; SSF51905; 1.
DR PROSITE; PS00977; FAD_G3PDH_1; 1.
PE 3: Inferred from homology;
KW Cell membrane; FAD; Flavoprotein; Glycerol metabolism; Membrane;
KW Oxidoreductase.
FT CHAIN 1..608
FT /note="Alpha-glycerophosphate oxidase"
FT /id="PRO_0000126108"
FT REGION 393..418
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 21..49
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255"
FT CONFLICT 100
FT /note="S -> P (in Ref. 1; AAC34740)"
FT /evidence="ECO:0000305"
FT CONFLICT 138
FT /note="A -> T (in Ref. 1; AAC34740)"
FT /evidence="ECO:0000305"
FT CONFLICT 275
FT /note="Y -> S (in Ref. 3; AAA26882)"
FT /evidence="ECO:0000305"
FT CONFLICT 367
FT /note="S -> R (in Ref. 3; AAA26882)"
FT /evidence="ECO:0000305"
FT CONFLICT 544
FT /note="T -> A (in Ref. 1; AAC34740)"
FT /evidence="ECO:0000305"
FT CONFLICT 572
FT /note="I -> V (in Ref. 1; AAC34740)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 608 AA; 66796 MW; A29B42FF4A75E406 CRC64;
MEFSKKTREL SIKKMQERTL DLLIIGGGIT GAGVALQAAA SGLETGLIEM QDFAEGTSSR
STKLVHGGLR YLKQFDVEVV SDTVSERAVV QQIAPHIPKS DPMLLPVYDE DGATFSLFRL
KVAMDLYDLL AGVSNTPAAN KVLSKDQVLE RQPNLKKEGL VGGGVYLDFR NNDARLVIEN
IKRANQDGAL IANHVKAEGF LFDESGKITG VVARDLLTDQ VFEIKARLVI NTTGPWSDKV
RNLSNKGTQF SQMRPTKGVH LVVDSSKIKV SQPVYFDTGL GDGRMVFVLP RENKTYFGTT
DTDYTGDLEH PKVTQEDVDY LLGIVNNRFP ESNITIDDIE SSWAGLRPLI AGNSASDYNG
GNNGTISDES FDNLIATVES YLSKEKTRED VESAVSKLES STSEKHLDPS AVSRGSSLDR
DDNGLLTLAG GKITDYRKMA EGAMERVVDI LKAEFDRSFK LINSKTYPVS GGELNPANVD
SEIEAFAQLG VSRGLDSKEA HYLANLYGSN APKVFALAHS LEQAPGLSLA DTLSLHYAMR
NELTLSPVDF LLRRTNHMLF MRDSLDSIVE PILDEMGRFY DWTEEEKATY RADVEAALAN
NDLAELKN
