ID   GLPO_STRPQ              Reviewed;         612 AA.
AC   P0DB21; Q8K666;
DT   27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=Alpha-glycerophosphate oxidase;
DE            EC=1.1.3.21;
DE   AltName: Full=Glycerol-3-phosphate oxidase;
GN   Name=glpO; OrderedLocusNames=SPs0399;
OS   Streptococcus pyogenes serotype M3 (strain SSI-1).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=193567;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SSI-1;
RX   PubMed=12799345; DOI=10.1101/gr.1096703;
RA   Nakagawa I., Kurokawa K., Yamashita A., Nakata M., Tomiyasu Y.,
RA   Okahashi N., Kawabata S., Yamazaki K., Shiba T., Yasunaga T., Hayashi H.,
RA   Hattori M., Hamada S.;
RT   "Genome sequence of an M3 strain of Streptococcus pyogenes reveals a large-
RT   scale genomic rearrangement in invasive strains and new insights into phage
RT   evolution.";
RL   Genome Res. 13:1042-1055(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=O2 + sn-glycerol 3-phosphate = dihydroxyacetone phosphate +
CC         H2O2; Xref=Rhea:RHEA:18369, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240,
CC         ChEBI:CHEBI:57597, ChEBI:CHEBI:57642; EC=1.1.3.21;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the FAD-dependent glycerol-3-phosphate
CC       dehydrogenase family. {ECO:0000305}.
CC   -!- CAUTION: As S.pyogenes is unable to produce acid from glycerol, the
CC       significance and/or function of the glpO gene in this organism is at
CC       present unknown. {ECO:0000305}.
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DR   EMBL; BA000034; BAC63494.1; -; Genomic_DNA.
DR   RefSeq; WP_011054908.1; NC_004606.1.
DR   AlphaFoldDB; P0DB21; -.
DR   SMR; P0DB21; -.
DR   KEGG; sps:SPs0399; -.
DR   HOGENOM; CLU_015740_5_2_9; -.
DR   OMA; CIVNAAG; -.
DR   GO; GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
DR   GO; GO:0004368; F:glycerol-3-phosphate dehydrogenase (quinone) activity; IEA:InterPro.
DR   GO; GO:0004369; F:glycerol-3-phosphate oxidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006072; P:glycerol-3-phosphate metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.8.870; -; 1.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR031656; DAO_C.
DR   InterPro; IPR038299; DAO_C_sf.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR000447; G3P_DH_FAD-dep.
DR   PANTHER; PTHR11985; PTHR11985; 1.
DR   Pfam; PF01266; DAO; 1.
DR   Pfam; PF16901; DAO_C; 1.
DR   PRINTS; PR01001; FADG3PDH.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   PROSITE; PS00977; FAD_G3PDH_1; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; FAD; Flavoprotein; Glycerol metabolism; Oxidoreductase.
FT   CHAIN           1..612
FT                   /note="Alpha-glycerophosphate oxidase"
FT                   /id="PRO_0000411348"
FT   REGION          398..418
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         21..49
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   612 AA;  67653 MW;  10BD47B2ED358B47 CRC64;
     MEFSRETRRL ALQKMQERDL DLLIIGGGIT GAGVALQAAA SGLDTGLIEM QDFAQGTSSR
     STKLVHGGLR YLKQFDVEVV SDTVSERAVV QQIAPHIPKP DPMLLPVYDE PGSTFSMFRL
     KVAMDLYDLL AGVSNTPAAN KVLTKEEVLK REPDLKQEGL LGGGVYLDFR NNDARLVIEN
     IKRANRDGAL IASHVKAEDF LLDDDGKIIG VKARDLLSDQ EIIIKAKLVI NTTGPWSDEI
     RQFSHKGQPI HQMRPTKGVH LVVDRQKLPV SQPVYVDTGL NDGRMVFVLP REEKTYFGTT
     DTDYTGDLEH PQVTQEDVDY LLGVVNNRFP NANVTIDDIE SSWAGLRPLL SGNSASDYNG
     GNSGKVSDDS FDHLVDTVKA YINHEDSREA VEKAIKQVET STSEKELDPS AVSRGSSFDR
     DENGLFTLAG GKITDYRKMA EGALTGIIQI LKEEFGKSFK LINSKTYPVS GGEINPANVD
     LEIEAYAQLG TLSGLSMDDA RYLANLYGSN APKVFALTRQ LTAAEGLSLA ETLSLHYAMD
     YEMALKPTDY FLRRTNHLLF MRDSLDALID PVINEMAKHF EWSDQERVAQ EDDLRRVIAD
     NDLSALKGHQ EG