ID   GLPQ1_MYCTO             Reviewed;         274 AA.
AC   P9WMU2; L0TGR7; P96236; Q7D4R6;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=Probable glycerophosphodiester phosphodiesterase 1;
DE            Short=Glycerophosphoryl diester phosphodiesterase 1;
DE            EC=3.1.4.46;
GN   Name=glpQ1; OrderedLocusNames=MT3950;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Glycerophosphoryl diester phosphodiesterase hydrolyzes
CC       deacylated phospholipids to G3P and the corresponding alcohols.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a sn-glycero-3-phosphodiester + H2O = an alcohol + H(+) + sn-
CC         glycerol 3-phosphate; Xref=Rhea:RHEA:12969, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30879, ChEBI:CHEBI:57597,
CC         ChEBI:CHEBI:83408; EC=3.1.4.46;
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC       Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the glycerophosphoryl diester phosphodiesterase
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK48317.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK48317.1; ALT_INIT; Genomic_DNA.
DR   PIR; G70653; G70653.
DR   RefSeq; WP_003420924.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WMU2; -.
DR   SMR; P9WMU2; -.
DR   EnsemblBacteria; AAK48317; AAK48317; MT3950.
DR   GeneID; 45427843; -.
DR   KEGG; mtc:MT3950; -.
DR   PATRIC; fig|83331.31.peg.4248; -.
DR   HOGENOM; CLU_030006_3_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0008889; F:glycerophosphodiester phosphodiesterase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR   Gene3D; 3.20.20.190; -; 1.
DR   InterPro; IPR030395; GP_PDE_dom.
DR   InterPro; IPR017946; PLC-like_Pdiesterase_TIM-brl.
DR   Pfam; PF03009; GDPD; 1.
DR   SUPFAM; SSF51695; SSF51695; 1.
DR   PROSITE; PS51704; GP_PDE; 1.
PE   3: Inferred from homology;
KW   Calcium; Glycerol metabolism; Hydrolase; Metal-binding.
FT   CHAIN           1..274
FT                   /note="Probable glycerophosphodiester phosphodiesterase 1"
FT                   /id="PRO_0000427242"
FT   DOMAIN          12..264
FT                   /note="GP-PDE"
FT   BINDING         44
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         46
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         126
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   274 AA;  29917 MW;  8EBC81ED9BFDCBDF CRC64;
     MTWADEVLAG HPFVVAHRGA SAARPEHTLA AYDLALKEGA DGVECDVRLT RDGHLVCVHD
     RRLDRTSTGA GLVSTMTLAQ LRELEYGAWH DSWRPDGSHG DTSLLTLDAL VSLVLDWHRP
     VKIFVETKHP VRYGSLVENK LLALLHRFGI AAPASADRSR AVVMSFSAAA VWRIRRAAPL
     LPTVLLGKTP RYLTSSAATA VGATAVGPSL PALKEYPQLV DRSAAQGRAV YCWNVDEYED
     IDFCREVGVA WIGTHHPGRT KAWLEDGRAN GTTR