ID   AMIB2_MYCBO             Reviewed;         462 AA.
AC   P63493; A0A1R3XY58; Q11056; X2BHG8;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Putative amidase AmiB2;
DE            EC=3.5.1.4;
GN   Name=amiB2; OrderedLocusNames=BQ2027_MB1294;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a monocarboxylic acid amide + H2O = a monocarboxylate +
CC         NH4(+); Xref=Rhea:RHEA:12020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:35757, ChEBI:CHEBI:83628; EC=3.5.1.4;
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
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DR   EMBL; LT708304; SIT99895.1; -; Genomic_DNA.
DR   RefSeq; NP_854948.1; NC_002945.3.
DR   RefSeq; WP_003406369.1; NC_002945.4.
DR   AlphaFoldDB; P63493; -.
DR   SMR; P63493; -.
DR   EnsemblBacteria; SIT99895; SIT99895; BQ2027_MB1294.
DR   GeneID; 45425233; -.
DR   PATRIC; fig|233413.5.peg.1419; -.
DR   OMA; NDGMGSL; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0004040; F:amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0043864; F:indoleacetamide hydrolase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR000120; Amidase.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR11895; PTHR11895; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   3: Inferred from homology;
KW   Hydrolase.
FT   CHAIN           1..462
FT                   /note="Putative amidase AmiB2"
FT                   /id="PRO_0000105256"
FT   ACT_SITE        81
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        155
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        179
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   462 AA;  49081 MW;  ACBF3B0CCEF64D58 CRC64;
     MDPTDLAFAG AAAQARMLAD GALTAPMLLE VYLQRIERLD SHLRAYRVVQ FDRARAEAEA
     AQQRLDAGER LPLLGVPIAI KDDVDIAGEV TTYGSAGHGP AATSDAEVVR RLRAAGAVII
     GKTNVPELMI MPFTESLAFG ATRNPWCLNR TPGGSSGGSA AAVAAGLAPV ALGSDGGGSI
     RIPCTWCGLF GLKPQRDRIS LEPHDGAWQG LSVNGPIARS VMDAALLLDA TTTVPGPEGE
     FVAAAARQPG RLRIALSTRV PTPLPVRCGK QELAAVHQAG ALLRDLGHDV VVRDPDYPAS
     TYANYLPRFF RGISDDADAQ AHPDRLEART RAIARLGSFF SDRRMAALRA AEVVLSSRIQ
     SIFDDVDVVV TPGAATGPSR IGAYQRRGAV STLLLVVQRV PYFQVWNLTG QPAAVVPWDF
     DGDGLPMSVQ LVGRPYDEAT LLALAAQIES ARPWAHRRPS VS