GLPR_ECOLI
ID GLPR_ECOLI Reviewed; 252 AA.
AC P0ACL0; P09392; Q2M787;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Glycerol-3-phosphate regulon repressor;
GN Name=glpR; OrderedLocusNames=b3423, JW3386;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-6.
RC STRAIN=K12;
RX PubMed=8955387; DOI=10.1128/jb.178.24.7080-7089.1996;
RA Zeng G., Ye S., Larson T.J.;
RT "Repressor for the sn-glycerol 3-phosphate regulon of Escherichia coli K-
RT 12: primary structure and identification of the DNA-binding domain.";
RL J. Bacteriol. 178:7080-7089(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=3045764; DOI=10.1093/nar/16.15.7732;
RA Choi Y.-L., Kawase S., Nishida T., Sakai H., Komano T., Kawamukai M.,
RA Utsumi R., Kohara Y., Akiyama K.;
RT "Nucleotide sequence of the glpR gene encoding the repressor for the
RT glycerol-3-phosphate regulon of Escherichia coli K12.";
RL Nucleic Acids Res. 16:7732-7732(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP SEQUENCE REVISION.
RC STRAIN=K12 / MG1655 / ATCC 700926;
RX PubMed=22081388; DOI=10.1128/jb.06087-11;
RA Freddolino P.L., Amini S., Tavazoie S.;
RT "Newly identified genetic variations in common Escherichia coli MG1655
RT stock cultures.";
RL J. Bacteriol. 194:303-306(2012).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP IDENTIFICATION BY 2D-GEL.
RX PubMed=9298644; DOI=10.1002/elps.1150180805;
RA VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT "Escherichia coli proteome analysis using the gene-protein database.";
RL Electrophoresis 18:1243-1251(1997).
CC -!- FUNCTION: Repressor of the glycerol-3-phosphate regulon.
CC -!- INTERACTION:
CC P0ACL0; P05847: ttdA; NbExp=2; IntAct=EBI-1123685, EBI-1113137;
CC -!- CAUTION: A pseudogene in strain MG1655 / ATCC 700926 due to a recent
CC laboratory-derived single nucleotide deletion, but not its parent
CC MG1655 / ATCC 47076. {ECO:0000305|PubMed:22081388}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA30399.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; M96795; AAC28167.1; -; Genomic_DNA.
DR EMBL; X07520; CAA30399.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U18997; AAA58221.1; -; Genomic_DNA.
DR EMBL; U00096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP009048; BAE77869.1; -; Genomic_DNA.
DR PIR; B65138; B65138.
DR RefSeq; WP_000815099.1; NZ_STEB01000004.1.
DR AlphaFoldDB; P0ACL0; -.
DR SMR; P0ACL0; -.
DR BioGRID; 4261187; 20.
DR DIP; DIP-9798N; -.
DR IntAct; P0ACL0; 2.
DR STRING; 316407.85676619; -.
DR SWISS-2DPAGE; P0ACL0; -.
DR jPOST; P0ACL0; -.
DR PaxDb; P0ACL0; -.
DR PRIDE; P0ACL0; -.
DR EnsemblBacteria; BAE77869; BAE77869; BAE77869.
DR GeneID; 67417050; -.
DR KEGG; ecj:JW3386; -.
DR PATRIC; fig|83333.103.peg.4322; -.
DR EchoBASE; EB0395; -.
DR eggNOG; COG1349; Bacteria.
DR HOGENOM; CLU_060699_0_0_6; -.
DR InParanoid; P0ACL0; -.
DR OMA; STHEPGF; -.
DR PhylomeDB; P0ACL0; -.
DR PRO; PR:P0ACL0; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR014036; DeoR_C.
DR InterPro; IPR001034; DeoR_HTH.
DR InterPro; IPR037171; NagB/RpiA_transferase-like.
DR InterPro; IPR018356; Tscrpt_reg_HTH_DeoR_CS.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00455; DeoRC; 1.
DR Pfam; PF08220; HTH_DeoR; 1.
DR PRINTS; PR00037; HTHLACR.
DR SMART; SM00420; HTH_DEOR; 1.
DR SUPFAM; SSF100950; SSF100950; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00894; HTH_DEOR_1; 1.
DR PROSITE; PS51000; HTH_DEOR_2; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; DNA-binding; Glycerol metabolism;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..252
FT /note="Glycerol-3-phosphate regulon repressor"
FT /id="PRO_0000050251"
FT DOMAIN 3..58
FT /note="HTH deoR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00349"
FT DNA_BIND 20..39
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00349"
FT CONFLICT 155
FT /note="A -> R (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 235
FT /note="P -> PP (in Ref. 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 252 AA; 28048 MW; D547569200AFB6B3 CRC64;
MKQTQRHNGI IELVKQQGYV STEELVEHFS VSPQTIRRDL NELAEQNLIL RHHGGAALPS
SSVNTPWHDR KATQTEEKER IARKVAEQIP NGSTLFIDIG TTPEAVAHAL LNHSNLRIVT
NNLNVANTLM VKEDFRIILA GGELRSRDGG IIGEATLDFI SQFRLDFGIL GISGIDSDGS
LLEFDYHEVR TKRAIIENSR HVMLVVDHSK FGRNAMVNMG SISMVDAVYT DAPPPVSVMQ
VLTDHHIQLE LC
