ID   AMIB2_MYCTO             Reviewed;         462 AA.
AC   P9WQ96; L0T8V5; P63492; Q11056;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Putative amidase AmiB2;
DE            EC=3.5.1.4;
GN   Name=amiB2; OrderedLocusNames=MT1301;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a monocarboxylic acid amide + H2O = a monocarboxylate +
CC         NH4(+); Xref=Rhea:RHEA:12020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:35757, ChEBI:CHEBI:83628; EC=3.5.1.4;
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE000516; AAK45560.1; -; Genomic_DNA.
DR   PIR; G70753; G70753.
DR   RefSeq; WP_003406369.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WQ96; -.
DR   SMR; P9WQ96; -.
DR   EnsemblBacteria; AAK45560; AAK45560; MT1301.
DR   GeneID; 45425233; -.
DR   KEGG; mtc:MT1301; -.
DR   PATRIC; fig|83331.31.peg.1405; -.
DR   HOGENOM; CLU_009600_0_4_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004040; F:amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0043864; F:indoleacetamide hydrolase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR000120; Amidase.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR11895; PTHR11895; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   3: Inferred from homology;
KW   Hydrolase.
FT   CHAIN           1..462
FT                   /note="Putative amidase AmiB2"
FT                   /id="PRO_0000426814"
FT   ACT_SITE        81
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        155
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        179
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   462 AA;  49081 MW;  ACBF3B0CCEF64D58 CRC64;
     MDPTDLAFAG AAAQARMLAD GALTAPMLLE VYLQRIERLD SHLRAYRVVQ FDRARAEAEA
     AQQRLDAGER LPLLGVPIAI KDDVDIAGEV TTYGSAGHGP AATSDAEVVR RLRAAGAVII
     GKTNVPELMI MPFTESLAFG ATRNPWCLNR TPGGSSGGSA AAVAAGLAPV ALGSDGGGSI
     RIPCTWCGLF GLKPQRDRIS LEPHDGAWQG LSVNGPIARS VMDAALLLDA TTTVPGPEGE
     FVAAAARQPG RLRIALSTRV PTPLPVRCGK QELAAVHQAG ALLRDLGHDV VVRDPDYPAS
     TYANYLPRFF RGISDDADAQ AHPDRLEART RAIARLGSFF SDRRMAALRA AEVVLSSRIQ
     SIFDDVDVVV TPGAATGPSR IGAYQRRGAV STLLLVVQRV PYFQVWNLTG QPAAVVPWDF
     DGDGLPMSVQ LVGRPYDEAT LLALAAQIES ARPWAHRRPS VS