ID   AMIB2_MYCTU             Reviewed;         462 AA.
AC   P9WQ97; L0T8V5; P63492; Q11056;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Putative amidase AmiB2;
DE            EC=3.5.1.4;
GN   Name=amiB2; OrderedLocusNames=Rv1263; ORFNames=MTCY50.19c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX   PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA   Raman K., Yeturu K., Chandra N.;
RT   "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT   through an interactome, reactome and genome-scale structural analysis.";
RL   BMC Syst. Biol. 2:109-109(2008).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a monocarboxylic acid amide + H2O = a monocarboxylate +
CC         NH4(+); Xref=Rhea:RHEA:12020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:35757, ChEBI:CHEBI:83628; EC=3.5.1.4;
CC   -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP44019.1; -; Genomic_DNA.
DR   PIR; G70753; G70753.
DR   RefSeq; NP_215779.1; NC_000962.3.
DR   RefSeq; WP_003406369.1; NZ_NVQJ01000049.1.
DR   AlphaFoldDB; P9WQ97; -.
DR   SMR; P9WQ97; -.
DR   STRING; 83332.Rv1263; -.
DR   PaxDb; P9WQ97; -.
DR   DNASU; 887041; -.
DR   GeneID; 45425233; -.
DR   GeneID; 887041; -.
DR   KEGG; mtu:Rv1263; -.
DR   TubercuList; Rv1263; -.
DR   eggNOG; COG0154; Bacteria.
DR   OMA; NDGMGSL; -.
DR   PhylomeDB; P9WQ97; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0004040; F:amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0043864; F:indoleacetamide hydrolase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR000120; Amidase.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR11895; PTHR11895; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..462
FT                   /note="Putative amidase AmiB2"
FT                   /id="PRO_0000105255"
FT   ACT_SITE        81
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        155
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        179
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   462 AA;  49081 MW;  ACBF3B0CCEF64D58 CRC64;
     MDPTDLAFAG AAAQARMLAD GALTAPMLLE VYLQRIERLD SHLRAYRVVQ FDRARAEAEA
     AQQRLDAGER LPLLGVPIAI KDDVDIAGEV TTYGSAGHGP AATSDAEVVR RLRAAGAVII
     GKTNVPELMI MPFTESLAFG ATRNPWCLNR TPGGSSGGSA AAVAAGLAPV ALGSDGGGSI
     RIPCTWCGLF GLKPQRDRIS LEPHDGAWQG LSVNGPIARS VMDAALLLDA TTTVPGPEGE
     FVAAAARQPG RLRIALSTRV PTPLPVRCGK QELAAVHQAG ALLRDLGHDV VVRDPDYPAS
     TYANYLPRFF RGISDDADAQ AHPDRLEART RAIARLGSFF SDRRMAALRA AEVVLSSRIQ
     SIFDDVDVVV TPGAATGPSR IGAYQRRGAV STLLLVVQRV PYFQVWNLTG QPAAVVPWDF
     DGDGLPMSVQ LVGRPYDEAT LLALAAQIES ARPWAHRRPS VS