GLR12_ARATH
ID GLR12_ARATH Reviewed; 867 AA.
AC Q9LV72; Q8LGN3;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 134.
DE RecName: Full=Glutamate receptor 1.2;
DE AltName: Full=Ligand-gated ion channel 1.2;
DE Flags: Precursor;
GN Name=GLR1.2; OrderedLocusNames=At5g48400; ORFNames=MJE7.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=12082126; DOI=10.1093/oxfordjournals.molbev.a004165;
RA Chiu J.C., Brenner E.D., DeSalle R., Nitabach M.N., Holmes T.C.,
RA Coruzzi G.M.;
RT "Phylogenetic and expression analysis of the glutamate-receptor-like gene
RT family in Arabidopsis thaliana.";
RL Mol. Biol. Evol. 19:1066-1082(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11379626; DOI=10.1126/science.292.5521.1486b;
RA Lacombe B., Becker D., Hedrich R., DeSalle R., Hollmann M., Kwak J.M.,
RA Schroeder J.I., Le Novere N., Nam H.G., Spalding E.P., Tester M.,
RA Turano F.J., Chiu J., Coruzzi G.;
RT "The identity of plant glutamate receptors.";
RL Science 292:1486-1487(2001).
CC -!- FUNCTION: Glutamate-gated receptor that probably acts as non-selective
CC cation channel. May be involved in light-signal transduction and
CC calcium homeostasis via the regulation of calcium influx into cells.
CC -!- SUBUNIT: May form heteromers. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=GLR1.2a;
CC IsoId=Q9LV72-1; Sequence=Displayed;
CC Name=2; Synonyms=GLR1.2b;
CC IsoId=Q9LV72-2; Sequence=VSP_009217, VSP_009218;
CC -!- TISSUE SPECIFICITY: Expressed predominantly in roots and siliques.
CC {ECO:0000269|PubMed:12082126}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to an intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10.1)
CC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY072064; AAL61993.1; -; mRNA.
DR EMBL; AY072065; AAL61994.1; -; mRNA.
DR EMBL; AB020745; BAA96960.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95665.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95666.1; -; Genomic_DNA.
DR RefSeq; NP_199651.1; NM_124215.2. [Q9LV72-1]
DR RefSeq; NP_851155.1; NM_180824.2. [Q9LV72-2]
DR AlphaFoldDB; Q9LV72; -.
DR SMR; Q9LV72; -.
DR BioGRID; 20141; 2.
DR IntAct; Q9LV72; 1.
DR STRING; 3702.AT5G48400.2; -.
DR PaxDb; Q9LV72; -.
DR PRIDE; Q9LV72; -.
DR ProteomicsDB; 247361; -. [Q9LV72-1]
DR EnsemblPlants; AT5G48400.1; AT5G48400.1; AT5G48400. [Q9LV72-2]
DR EnsemblPlants; AT5G48400.2; AT5G48400.2; AT5G48400. [Q9LV72-1]
DR GeneID; 834895; -.
DR Gramene; AT5G48400.1; AT5G48400.1; AT5G48400. [Q9LV72-2]
DR Gramene; AT5G48400.2; AT5G48400.2; AT5G48400. [Q9LV72-1]
DR KEGG; ath:AT5G48400; -.
DR Araport; AT5G48400; -.
DR TAIR; locus:2166001; AT5G48400.
DR eggNOG; KOG1052; Eukaryota.
DR HOGENOM; CLU_007358_0_0_1; -.
DR InParanoid; Q9LV72; -.
DR OMA; SFWFTLG; -.
DR OrthoDB; 188544at2759; -.
DR PhylomeDB; Q9LV72; -.
DR PRO; PR:Q9LV72; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LV72; baseline and differential.
DR Genevisible; Q9LV72; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0005262; F:calcium channel activity; ISS:UniProtKB.
DR GO; GO:0008066; F:glutamate receptor activity; ISS:UniProtKB.
DR GO; GO:0015276; F:ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0038023; F:signaling receptor activity; IBA:GO_Central.
DR GO; GO:0006816; P:calcium ion transport; ISS:UniProtKB.
DR GO; GO:0019722; P:calcium-mediated signaling; ISS:UniProtKB.
DR GO; GO:0071230; P:cellular response to amino acid stimulus; ISS:UniProtKB.
DR CDD; cd19990; PBP1_GABAb_receptor_plant; 1.
DR InterPro; IPR001828; ANF_lig-bd_rcpt.
DR InterPro; IPR044440; GABAb_receptor_plant_PBP1.
DR InterPro; IPR001320; Iontro_rcpt.
DR InterPro; IPR017103; Iontropic_Glu_rcpt_pln.
DR InterPro; IPR028082; Peripla_BP_I.
DR InterPro; IPR001638; Solute-binding_3/MltF_N.
DR Pfam; PF01094; ANF_receptor; 1.
DR Pfam; PF00060; Lig_chan; 1.
DR Pfam; PF00497; SBP_bac_3; 1.
DR PIRSF; PIRSF037090; Iontro_Glu-like_rcpt_pln; 1.
DR SMART; SM00079; PBPe; 1.
DR SUPFAM; SSF53822; SSF53822; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Ion channel; Ion transport;
KW Ligand-gated ion channel; Membrane; Receptor; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..867
FT /note="Glutamate receptor 1.2"
FT /id="PRO_0000011593"
FT TOPO_DOM 28..560
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 561..581
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 582..590
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 591..611
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 612..622
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 623..643
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 644..792
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 793..813
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 814..867
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 301
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 400
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 496
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 499
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 676
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 688
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 699
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 748
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 738..750
FT /note="MFQKGSELAPNVS -> VCILLLHHQLLYS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12082126"
FT /id="VSP_009217"
FT VAR_SEQ 751..867
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12082126"
FT /id="VSP_009218"
SQ SEQUENCE 867 AA; 98152 MW; 2AC7E991CEE16690 CRC64;
MVRICIQTPI LLSFLLVLLF FISNCFASSQ NNDDDKRIRV RVGLVLDLGS VEGKIVRSSV
SMALSDFYDN HNDYKTRLSL LVRDSHGEPL LALDSVVDLL QTEGVQAIIG GNSLLEAKLL
AELGEKARVP VISLNSPMSL SLSKYTHLIQ ATHNSASEVK GITAFLHGFD WNSVALVLED
HDDWRESMHF MVDHFHENNV HVQSKVAFSV TSSEDSLMDR LRELKDLGTT VFVVHLSEVI
ATRLFPCAEK LGMMGEGFAW ILTSRSMSSF HDQFIDDLTK EAMEGVVGFK SYIPMSKELH
NFTLRWRKTL PVEEVTGSEI TRLSISGVWA HDVAWSLASA AEVTRMPTVT STLLEAIKES
RFKGLSGNFQ LDDMKLLSDK FEIVNMIGSG ERRVGFWNSN GSFSNRRQLS STHDNLETII
WPGGSAQSPK GRSLRESGRK KLRVLVTSSN RFPRLMKVET DPITHEITIV EGFCIEVFQA
SIAPFNYEVE YIRWLNGTNY TKLAYALHSQ KDKYDAAVGD ITITSDRSMY VDFTLPYTEM
GLGIVAAKER SMWVFFQPLT PNLWITSAAF FVLTGIIVWL IERAENKEFQ GSWPQQIGVV
IWFGFSTLVY AHREKLQHNL SRFVVTVWVF AVLILVTSYT ATLTSMMTVQ QIRFNANEDY
VGHLSGSLIA NAALTNSSLR AMRLLGLNTS EDYAQALMNK SVSYIVSELP YLKILLGENP
GHFLMVKTQS TTNGFGFMFQ KGSELAPNVS REIAKLRTSE RLNEMERRWF DKQLPYTTDD
TSNPITLYRF RGLFMITGVS FAFALAVLLI LWLRERWEIL VNSVNIYFSQ RLRHFRILFT
RTIHPSPLGL DNPIGENAVQ MAQRNRR
