GLR14_ARATH
ID GLR14_ARATH Reviewed; 861 AA.
AC Q8LGN1; Q8LGN2; Q9SRR4;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 16-JAN-2004, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Glutamate receptor 1.4;
DE AltName: Full=Ligand-gated ion channel 1.4;
DE Flags: Precursor;
GN Name=GLR1.4; OrderedLocusNames=At3g07520; ORFNames=F21O3.23;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=12082126; DOI=10.1093/oxfordjournals.molbev.a004165;
RA Chiu J.C., Brenner E.D., DeSalle R., Nitabach M.N., Holmes T.C.,
RA Coruzzi G.M.;
RT "Phylogenetic and expression analysis of the glutamate-receptor-like gene
RT family in Arabidopsis thaliana.";
RL Mol. Biol. Evol. 19:1066-1082(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11379626; DOI=10.1126/science.292.5521.1486b;
RA Lacombe B., Becker D., Hedrich R., DeSalle R., Hollmann M., Kwak J.M.,
RA Schroeder J.I., Le Novere N., Nam H.G., Spalding E.P., Tester M.,
RA Turano F.J., Chiu J., Coruzzi G.;
RT "The identity of plant glutamate receptors.";
RL Science 292:1486-1487(2001).
RN [5]
RP FUNCTION.
RX PubMed=18625242; DOI=10.1016/j.jmb.2008.06.076;
RA Tapken D., Hollmann M.;
RT "Arabidopsis thaliana glutamate receptor ion channel function demonstrated
RT by ion pore transplantation.";
RL J. Mol. Biol. 383:36-48(2008).
CC -!- FUNCTION: Glutamate-gated receptor that probably acts as non-selective
CC cation channel. Can transport calcium ions. May be involved in light-
CC signal transduction and calcium homeostasis via the regulation of
CC calcium influx into cells. {ECO:0000269|PubMed:18625242}.
CC -!- SUBUNIT: May form heteromers. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed predominantly in roots and siliques.
CC {ECO:0000269|PubMed:12082126}.
CC -!- SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10.1)
CC family. {ECO:0000305}.
CC -!- CAUTION: AAL61996 is a fragment and was originally reported as a
CC spliced variant of AAL61995. {ECO:0000305|PubMed:12082126}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF02156.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY072066; AAL61995.1; -; mRNA.
DR EMBL; AY072067; AAL61996.1; -; mRNA.
DR EMBL; AC009853; AAF02156.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE74553.1; -; Genomic_DNA.
DR RefSeq; NP_187408.2; NM_111630.2.
DR AlphaFoldDB; Q8LGN1; -.
DR SMR; Q8LGN1; -.
DR BioGRID; 5275; 11.
DR STRING; 3702.AT3G07520.1; -.
DR iPTMnet; Q8LGN1; -.
DR PaxDb; Q8LGN1; -.
DR PRIDE; Q8LGN1; -.
DR EnsemblPlants; AT3G07520.1; AT3G07520.1; AT3G07520.
DR GeneID; 819940; -.
DR Gramene; AT3G07520.1; AT3G07520.1; AT3G07520.
DR KEGG; ath:AT3G07520; -.
DR Araport; AT3G07520; -.
DR TAIR; locus:2079681; AT3G07520.
DR eggNOG; KOG1052; Eukaryota.
DR HOGENOM; CLU_007358_0_0_1; -.
DR InParanoid; Q8LGN1; -.
DR OMA; QQNAIHI; -.
DR OrthoDB; 188544at2759; -.
DR PhylomeDB; Q8LGN1; -.
DR PRO; PR:Q8LGN1; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8LGN1; baseline and differential.
DR Genevisible; Q8LGN1; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0005262; F:calcium channel activity; IDA:UniProtKB.
DR GO; GO:0005261; F:cation channel activity; IDA:TAIR.
DR GO; GO:0008066; F:glutamate receptor activity; ISS:UniProtKB.
DR GO; GO:0015276; F:ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0038023; F:signaling receptor activity; IBA:GO_Central.
DR GO; GO:0006816; P:calcium ion transport; IDA:UniProtKB.
DR GO; GO:0019722; P:calcium-mediated signaling; ISS:UniProtKB.
DR GO; GO:0030003; P:cellular cation homeostasis; IDA:TAIR.
DR GO; GO:0071230; P:cellular response to amino acid stimulus; ISS:UniProtKB.
DR CDD; cd19990; PBP1_GABAb_receptor_plant; 1.
DR InterPro; IPR001828; ANF_lig-bd_rcpt.
DR InterPro; IPR044440; GABAb_receptor_plant_PBP1.
DR InterPro; IPR001320; Iontro_rcpt.
DR InterPro; IPR017103; Iontropic_Glu_rcpt_pln.
DR InterPro; IPR028082; Peripla_BP_I.
DR InterPro; IPR001638; Solute-binding_3/MltF_N.
DR Pfam; PF01094; ANF_receptor; 1.
DR Pfam; PF00060; Lig_chan; 1.
DR Pfam; PF00497; SBP_bac_3; 1.
DR PIRSF; PIRSF037090; Iontro_Glu-like_rcpt_pln; 1.
DR SMART; SM00079; PBPe; 1.
DR SUPFAM; SSF53822; SSF53822; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Ion channel; Ion transport; Ligand-gated ion channel;
KW Membrane; Receptor; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix; Transport.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..861
FT /note="Glutamate receptor 1.4"
FT /id="PRO_0000011595"
FT TOPO_DOM 30..568
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 569..589
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 590..598
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 599..619
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 620..630
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 631..651
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 652..800
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 801..821
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 822..861
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 306
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 534
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 707
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 861 AA; 97760 MW; 0782EE543B78BA63 CRC64;
MENCMIRNTG YFLTIFFLAF ISFAVTCSGT NQKDNVDRLP VVYEDVRIGL VVDMGSMEGK
LVTTSISMAL SDFYHVNNGY RTRVSVLSRD SHGDPLQALA AAMDLLQTEQ VEALVGGQSL
LEAKNLAELG EKTKVPVISS FQVPSSLSLA KYNYFIQATH DTSSEAKGIA ALFSNFDWRT
AVLIYEDDDD WRESIQPLVG HFQQNAIHIE YKAEFSVSSN EECIMKQLRK FKASGIRIFV
AHISERIANR LFPCARRLGM MEEGYAWILT ARSMNNFQDT NYLAKEEMEG VIGFKSYIPL
TEELHNFTLR WKRSLRLEEV VTRMSVCSIW AHDIAWSLAR AAEVAKLPGL SVYDLLEAIP
ESAKHKGLSG DIKFIDKKFI SDKFEIVNMI GRGERSVGLW NSGSFISNRR RRLSSTKALE
TIIWPGGSTR IPKIRSLKEK RHGKKKKLRV LVPAGNITPQ ILEVKTDFKT GVTAATGYCI
DVFETSILPF NYEVEYIPWP GAINYKNYND LVYTLYSQKD KYDAAVGDIT ITDNRSLYVD
FTLPFTDMGL AVVTAKDKSM WIIFKPLTLS LWLTIASFFI LTGAIVWLIE RHDNADFQGS
CFQQIGTLLC FGFSTLVFAH RERLQHNMSR FVVIVWIFAV LILTSNYTAT LTSVMTVQQI
RGLKSNENIG FFSASIAANV VNDNPTFQGP RYKGLKTADD FTNALRNGTI SFIVDEVPYV
KLFVAKHPSE FVIVETESVT NGFGFAFQKG SPLVQKVSRE IEKLRRTEKL KAIENWWFQR
QTTSATSEDT FHPLTVYTFR GLFMITGVSF AFALIVYLIP WNREQRQVVL KHFHRYVSHR
FAREIRPSPT TPNRQNENSV I
