GLR1_DROME
ID GLR1_DROME Reviewed; 991 AA.
AC Q03445; B6IDJ5; Q86PC2; Q9VRX9;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 21-JUN-2005, sequence version 2.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=Glutamate receptor 1;
DE AltName: Full=Glutamate receptor I;
DE Short=dGLUR-I;
DE AltName: Full=Kainate-selective glutamate receptor;
DE Flags: Precursor;
GN Name=GluRIA; Synonyms=Glu-RI; ORFNames=CG8442;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=Canton-S; TISSUE=Embryo;
RX PubMed=1359540; DOI=10.1073/pnas.89.21.10484;
RA Ultsch A., Schuster C.M., Laube B., Schloss P., Schmitt B., Betz H.;
RT "Glutamate receptors of Drosophila melanogaster: cloning of a kainate-
RT selective subunit expressed in the central nervous system.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:10484-10488(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Booth B.,
RA Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E.,
RA George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G.,
RA Miranda A., Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S.,
RA Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M.,
RA Celniker S.E.;
RL Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Receptor for glutamate. L-glutamate acts as an excitatory
CC neurotransmitter at many synapses in the central nervous system. The
CC postsynaptic actions of Glu are mediated by a variety of receptors that
CC are named according to their selective agonists (By similarity). Forms
CC ligand-gated ion channels which are activated by kainate. {ECO:0000250,
CC ECO:0000269|PubMed:1359540}.
CC -!- SUBUNIT: Homooligomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:1359540};
CC Multi-pass membrane protein {ECO:0000269|PubMed:1359540}. Postsynaptic
CC cell membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=A;
CC IsoId=Q03445-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q03445-2; Sequence=VSP_014219;
CC -!- TISSUE SPECIFICITY: Central nervous system.
CC {ECO:0000269|PubMed:1359540}.
CC -!- DEVELOPMENTAL STAGE: No expression is seen in early embryogenesis,
CC whereas high expression occurs in late embryos. During larval
CC development, expression decreases to undetectable levels in late
CC larvae, resumes at the early pupal stage and gradually increases in
CC late pupae and early adult flies. High levels of expression coincide
CC with major stages of neurogenesis. {ECO:0000269|PubMed:1359540}.
CC -!- SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10.1)
CC family. {ECO:0000305}.
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DR EMBL; M97192; AAA28575.1; -; mRNA.
DR EMBL; AE014296; AAF50652.2; -; Genomic_DNA.
DR EMBL; BT003218; AAO24973.1; -; mRNA.
DR EMBL; BT050435; ACJ13142.1; -; mRNA.
DR PIR; A46421; A46421.
DR RefSeq; NP_476855.1; NM_057507.4. [Q03445-1]
DR PDB; 5DT6; X-ray; 1.60 A; A=474-594, A=739-880.
DR PDB; 5ICT; X-ray; 1.68 A; A=474-594, A=739-880.
DR PDBsum; 5DT6; -.
DR PDBsum; 5ICT; -.
DR AlphaFoldDB; Q03445; -.
DR SMR; Q03445; -.
DR BioGRID; 64192; 1.
DR IntAct; Q03445; 1.
DR STRING; 7227.FBpp0076691; -.
DR GlyGen; Q03445; 9 sites.
DR PaxDb; Q03445; -.
DR PRIDE; Q03445; -.
DR EnsemblMetazoa; FBtr0076982; FBpp0076691; FBgn0004619. [Q03445-1]
DR GeneID; 38742; -.
DR KEGG; dme:Dmel_CG8442; -.
DR CTD; 38742; -.
DR FlyBase; FBgn0004619; GluRIA.
DR VEuPathDB; VectorBase:FBgn0004619; -.
DR eggNOG; KOG1054; Eukaryota.
DR GeneTree; ENSGT00940000168258; -.
DR HOGENOM; CLU_007257_1_2_1; -.
DR InParanoid; Q03445; -.
DR OMA; VEFFCRS; -.
DR PhylomeDB; Q03445; -.
DR Reactome; R-DME-204005; COPII-mediated vesicle transport.
DR Reactome; R-DME-399710; Activation of AMPA receptors.
DR Reactome; R-DME-438066; Unblocking of NMDA receptors, glutamate binding and activation.
DR Reactome; R-DME-451308; Activation of Ca-permeable Kainate Receptor.
DR Reactome; R-DME-5694530; Cargo concentration in the ER.
DR BioGRID-ORCS; 38742; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 38742; -.
DR PRO; PR:Q03445; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0004619; Expressed in brain and 19 other tissues.
DR Genevisible; Q03445; DM.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0008328; C:ionotropic glutamate receptor complex; IC:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015277; F:kainate selective glutamate receptor activity; IDA:FlyBase.
DR GO; GO:0015276; F:ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0038023; F:signaling receptor activity; IBA:GO_Central.
DR GO; GO:0006812; P:cation transport; IDA:FlyBase.
DR InterPro; IPR001828; ANF_lig-bd_rcpt.
DR InterPro; IPR019594; Glu/Gly-bd.
DR InterPro; IPR001320; Iontro_rcpt.
DR InterPro; IPR028082; Peripla_BP_I.
DR Pfam; PF01094; ANF_receptor; 1.
DR Pfam; PF00060; Lig_chan; 1.
DR Pfam; PF10613; Lig_chan-Glu_bd; 1.
DR SMART; SM00918; Lig_chan-Glu_bd; 1.
DR SMART; SM00079; PBPe; 1.
DR SUPFAM; SSF53822; SSF53822; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell membrane; Glycoprotein;
KW Ion channel; Ion transport; Ligand-gated ion channel; Membrane;
KW Postsynaptic cell membrane; Receptor; Reference proteome; Signal; Synapse;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..991
FT /note="Glutamate receptor 1"
FT /id="PRO_0000011555"
FT TOPO_DOM 28..611
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 612..632
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 633..710
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 711..731
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 732..895
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 896..916
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 917..991
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 300..321
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 354..379
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..321
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 360..379
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 67
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 195
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 208
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 281
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 376
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 385
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 426
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 437
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 477
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..265
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_014219"
FT CONFLICT 61
FT /note="M -> L (in Ref. 1; AAA28575)"
FT /evidence="ECO:0000305"
FT CONFLICT 301
FT /note="S -> N (in Ref. 1; AAA28575)"
FT /evidence="ECO:0000305"
FT CONFLICT 309..316
FT /note="GQSQSQNA -> AKAKARTQ (in Ref. 1; AAA28575)"
FT /evidence="ECO:0000305"
FT CONFLICT 324
FT /note="A -> P (in Ref. 1; AAA28575)"
FT /evidence="ECO:0000305"
FT CONFLICT 747
FT /note="T -> A (in Ref. 1; AAA28575)"
FT /evidence="ECO:0000305"
FT CONFLICT 759
FT /note="Y -> H (in Ref. 1; AAA28575)"
FT /evidence="ECO:0000305"
FT CONFLICT 916
FT /note="M -> V (in Ref. 1; AAA28575)"
FT /evidence="ECO:0000305"
FT CONFLICT 944
FT /note="A -> G (in Ref. 1; AAA28575)"
FT /evidence="ECO:0000305"
FT STRAND 476..484
FT /evidence="ECO:0007829|PDB:5DT6"
FT TURN 488..490
FT /evidence="ECO:0007829|PDB:5DT6"
FT STRAND 491..493
FT /evidence="ECO:0007829|PDB:5DT6"
FT HELIX 504..507
FT /evidence="ECO:0007829|PDB:5DT6"
FT STRAND 508..510
FT /evidence="ECO:0007829|PDB:5DT6"
FT HELIX 511..523
FT /evidence="ECO:0007829|PDB:5DT6"
FT STRAND 526..531
FT /evidence="ECO:0007829|PDB:5DT6"
FT HELIX 550..556
FT /evidence="ECO:0007829|PDB:5DT6"
FT STRAND 561..563
FT /evidence="ECO:0007829|PDB:5DT6"
FT HELIX 571..574
FT /evidence="ECO:0007829|PDB:5DT6"
FT STRAND 577..579
FT /evidence="ECO:0007829|PDB:5DT6"
FT STRAND 583..586
FT /evidence="ECO:0007829|PDB:5DT6"
FT STRAND 588..593
FT /evidence="ECO:0007829|PDB:5DT6"
FT HELIX 743..746
FT /evidence="ECO:0007829|PDB:5DT6"
FT STRAND 753..755
FT /evidence="ECO:0007829|PDB:5DT6"
FT HELIX 761..768
FT /evidence="ECO:0007829|PDB:5DT6"
FT HELIX 772..783
FT /evidence="ECO:0007829|PDB:5DT6"
FT HELIX 784..787
FT /evidence="ECO:0007829|PDB:5DT6"
FT STRAND 788..791
FT /evidence="ECO:0007829|PDB:5DT6"
FT HELIX 792..801
FT /evidence="ECO:0007829|PDB:5DT6"
FT TURN 802..804
FT /evidence="ECO:0007829|PDB:5DT6"
FT STRAND 806..811
FT /evidence="ECO:0007829|PDB:5DT6"
FT HELIX 812..819
FT /evidence="ECO:0007829|PDB:5DT6"
FT STRAND 826..830
FT /evidence="ECO:0007829|PDB:5DT6"
FT STRAND 836..838
FT /evidence="ECO:0007829|PDB:5DT6"
FT STRAND 841..843
FT /evidence="ECO:0007829|PDB:5DT6"
FT HELIX 849..861
FT /evidence="ECO:0007829|PDB:5DT6"
FT HELIX 864..872
FT /evidence="ECO:0007829|PDB:5DT6"
FT HELIX 874..876
FT /evidence="ECO:0007829|PDB:5DT6"
SQ SEQUENCE 991 AA; 111668 MW; C8917868ABA06F98 CRC64;
MHSRLKFLAY LHFICASSIF WPEFSSAQQQ QQTVSLTEKI PLGAIFEQGT DDVQSAFKYA
MLNHNLNVSS RRFELQAYVD VINTADAFKL SRLICNQFSR GVYSMLGAVS PDSFDTLHSY
SNTFQMPFVT PWFPEKVLAP SSGLLDFAIS MRPDYHQAII DTIQYYGWQS IIYLYDSHDG
LLRLQQIYQE LKPGNETFRV QMVKRIANVT MAIEFLHTLE DLGRFSKKRI VLDCPAEMAK
EIIVQHVRDI KLGRRTYHYL LSGLVMDNHW PSDVVEFGAI NITGFRIVDS NRRAVRDFHD
SRKRLEPSGQ SQSQNAGGPN SLPAISAQAA LMYDAVFVLV EAFNRILRKK PDQFRSNHLQ
RRSHGGSSSS SATGTNESSA LLDCNTSKGW VTPWEQGEKI SRVLRKVEID GLSGEIRFDE
DGRRINYTLH VVEMSVNSTL QQVAEWRDDA GLLPLHSHNY ASSSRSASAS TGDYDRNHTY
IVSSLLEEPY LSLKQYTYGE SLVGNDRFEG YCKDLADMLA AQLGIKYEIR LVQDGNYGAE
NQYAPGGWDG MVGELIRKEA DIAISAMTIT AERERVIDFS KPFMTLGISI MIKKPVKQTP
GVFSFLNPLS QEIWISVILS YVGVSFVLYF VTRFPPYEWR IVRRPQADST AQQPPGIIGG
ATLSEPQAHV PPVPPNEFTM LNSFWYSLAA FMQQGCDITP PSIAGRIAAA VWWFFTIILI
SSYTANLAAF LTVERMVAPI KTPEDLTMQT DVNYGTLLYG STWEFFRRSQ IGLHNKMWEY
MNANQHHSVH TYDEGIRRVR QSKGKYALLV ESPKNEYVNA RPPCDTMKVG RNIDTKGFGV
ATPIGSPLRK RLNEAVLTLK ENGELLRIRN KWWFDKTECN LDQETSTPNE LSLSNVAGIY
YILIGGLLLA VIVAIMEFFC RNKTPQLKSP GSNGSAGGVP GMLASSTYQR DSLSDAIMHS
QAKLAMQASS EYDERLVGVE LASNVRYQYS M
