ID   AMIB_BUCAP              Reviewed;         233 AA.
AC   Q8K908;
DT   08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Putative N-acetylmuramoyl-L-alanine amidase;
DE            EC=3.5.1.28;
GN   Name=amiB; OrderedLocusNames=BUsg_555;
OS   Buchnera aphidicola subsp. Schizaphis graminum (strain Sg).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=198804;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sg;
RX   PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- FUNCTION: Cell-wall hydrolase involved in septum cleavage during cell
CC       division. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolyzes the link between N-acetylmuramoyl residues and L-
CC         amino acid residues in certain cell-wall glycopeptides.; EC=3.5.1.28;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.
CC       {ECO:0000305}.
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DR   EMBL; AE013218; AAM68093.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8K908; -.
DR   SMR; Q8K908; -.
DR   STRING; 198804.BUsg_555; -.
DR   EnsemblBacteria; AAM68093; AAM68093; BUsg_555.
DR   KEGG; bas:BUsg_555; -.
DR   eggNOG; COG0860; Bacteria.
DR   HOGENOM; CLU_014322_4_1_6; -.
DR   OMA; QIRPVHH; -.
DR   Proteomes; UP000000416; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008745; F:N-acetylmuramoyl-L-alanine amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009253; P:peptidoglycan catabolic process; IEA:InterPro.
DR   CDD; cd02696; MurNAc-LAA; 1.
DR   InterPro; IPR002508; MurNAc-LAA_cat.
DR   Pfam; PF01520; Amidase_3; 1.
DR   SMART; SM00646; Ami_3; 1.
PE   3: Inferred from homology;
KW   Cell wall biogenesis/degradation; Hydrolase; Secreted.
FT   CHAIN           1..233
FT                   /note="Putative N-acetylmuramoyl-L-alanine amidase"
FT                   /id="PRO_0000164422"
FT   DOMAIN          1..219
FT                   /note="MurNAc-LAA"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   233 AA;  27259 MW;  4F492C6C2D5FF31E CRC64;
     MIDPGHGGQD PGAINSLGLQ EKKITLKIGI KLKNLLQNSD LFYPVLTRND DSYVSLKKRR
     DFLKNNHVSF LISIHADSSK KRYVSGASIW ITTNDRMHRE INNFIKNREE NIYFPKNIQN
     LIQKNKHDFF LKKTVLDLQF NNFQKMEINL SRYIFQQLKK IIKLDKINLN YASLGILSSI
     NTPSMLIETG FITNFLEEKK LRTNKYQNKI ANAIYIALKN YFQDRLLSNL RNT