GLR25_ARATH
ID GLR25_ARATH Reviewed; 918 AA.
AC Q9LFN5; F4JWF9;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 16-JAN-2004, sequence version 2.
DT 25-MAY-2022, entry version 141.
DE RecName: Full=Glutamate receptor 2.5;
DE AltName: Full=Ligand-gated ion channel 2.5;
DE Flags: Precursor;
GN Name=GLR2.5; OrderedLocusNames=At5g11210; ORFNames=F2I11_100;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11379626; DOI=10.1126/science.292.5521.1486b;
RA Lacombe B., Becker D., Hedrich R., DeSalle R., Hollmann M., Kwak J.M.,
RA Schroeder J.I., Le Novere N., Nam H.G., Spalding E.P., Tester M.,
RA Turano F.J., Chiu J., Coruzzi G.;
RT "The identity of plant glutamate receptors.";
RL Science 292:1486-1487(2001).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=12082126; DOI=10.1093/oxfordjournals.molbev.a004165;
RA Chiu J.C., Brenner E.D., DeSalle R., Nitabach M.N., Holmes T.C.,
RA Coruzzi G.M.;
RT "Phylogenetic and expression analysis of the glutamate-receptor-like gene
RT family in Arabidopsis thaliana.";
RL Mol. Biol. Evol. 19:1066-1082(2002).
CC -!- FUNCTION: Glutamate-gated receptor that probably acts as non-selective
CC cation channel. May be involved in light-signal transduction and
CC calcium homeostasis via the regulation of calcium influx into cells.
CC -!- SUBUNIT: May form heteromers. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed predominantly in roots.
CC {ECO:0000269|PubMed:12082126}.
CC -!- SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10.1)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB96656.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL360314; CAB96656.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; ANM70627.1; -; Genomic_DNA.
DR RefSeq; NP_001332219.1; NM_001343168.1.
DR AlphaFoldDB; Q9LFN5; -.
DR SMR; Q9LFN5; -.
DR STRING; 3702.AT5G11210.1; -.
DR PaxDb; Q9LFN5; -.
DR ProteomicsDB; 248591; -.
DR EnsemblPlants; AT5G11210.5; AT5G11210.5; AT5G11210.
DR GeneID; 830991; -.
DR Gramene; AT5G11210.5; AT5G11210.5; AT5G11210.
DR KEGG; ath:AT5G11210; -.
DR Araport; AT5G11210; -.
DR eggNOG; KOG1052; Eukaryota.
DR HOGENOM; CLU_007358_0_1_1; -.
DR InParanoid; Q9LFN5; -.
DR OrthoDB; 188544at2759; -.
DR PhylomeDB; Q9LFN5; -.
DR PRO; PR:Q9LFN5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LFN5; baseline and differential.
DR Genevisible; Q9LFN5; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0005262; F:calcium channel activity; ISS:UniProtKB.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IEA:InterPro.
DR GO; GO:0008066; F:glutamate receptor activity; ISS:UniProtKB.
DR GO; GO:0015276; F:ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0038023; F:signaling receptor activity; IBA:GO_Central.
DR GO; GO:0006816; P:calcium ion transport; ISS:UniProtKB.
DR GO; GO:0019722; P:calcium-mediated signaling; ISS:UniProtKB.
DR GO; GO:0071230; P:cellular response to amino acid stimulus; ISS:UniProtKB.
DR CDD; cd19990; PBP1_GABAb_receptor_plant; 1.
DR InterPro; IPR001828; ANF_lig-bd_rcpt.
DR InterPro; IPR044440; GABAb_receptor_plant_PBP1.
DR InterPro; IPR000337; GPCR_3.
DR InterPro; IPR001320; Iontro_rcpt.
DR InterPro; IPR017103; Iontropic_Glu_rcpt_pln.
DR InterPro; IPR028082; Peripla_BP_I.
DR InterPro; IPR001638; Solute-binding_3/MltF_N.
DR Pfam; PF01094; ANF_receptor; 1.
DR Pfam; PF00060; Lig_chan; 1.
DR Pfam; PF00497; SBP_bac_3; 1.
DR PIRSF; PIRSF037090; Iontro_Glu-like_rcpt_pln; 1.
DR PRINTS; PR00248; GPCRMGR.
DR SMART; SM00079; PBPe; 1.
DR SUPFAM; SSF53822; SSF53822; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Ion channel; Ion transport; Ligand-gated ion channel;
KW Membrane; Receptor; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix; Transport.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..918
FT /note="Glutamate receptor 2.5"
FT /id="PRO_0000011600"
FT TOPO_DOM 31..586
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 587..607
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 608..616
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 617..637
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 638..647
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 648..668
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 669..828
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 829..849
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 850..918
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 857..881
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 857..875
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 46
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 58
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 122
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 336
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 340
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 546
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 791
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 918 AA; 103541 MW; 75E0C00D1900D3F9 CRC64;
MSLFHHLVSR FLSLWLLIFL VFLVLSLGKS QKEALQVKVG IVLGSNVTLA DLSLRAINMS
LSEFYNTHNG FKTRIVLNVR DSKQTVVGAA ASALYLIKKR EVVAIIGPGT SMQAPFLINL
GNQSKVPIIS FSATSPLLDS LRSPYFIRAT HDDSSQVQAI SAIIESFRWR EVVPIYVDNE
FGEGILPNLV DAFQEINVRI RYRSAISLHY SDDQIKKELY KLMTMPTRVF IVHMLPDLGS
RLFSIAKEID MLSKGYVWIV TNGIADLMSI MGESSLVNMH GVLGVKTYFA KSKELLHLEA
RWQKRFGGEE LNNFACWAYD AATALAMSVE EIRHVNMSFN TTKEDTSRDD IGTDLDELGV
ALSGPKLLDA LSTVSFKGVA GRFQLKNGKL EATTFKIINI EESGERTVGF WKSKVGLVKS
LRVDKVSHSS RRLRPIIWPG DTIFVPKGWE FPTNAKKLRI AVPKKDGFNN FVEVTKDENT
NVPTVTGFCI DVFNTVMSQM PYAVSYEYIP FDTPDGKPRG SYDEMVYNVF LGEFDGAVGD
TTILANRSHY VDFALPYSET GIVFLVPVKD GKEKGEWVFL KPLTKELWLV TAASFLYIGI
MVWIFEYQAD EEFREQMIID KISSVFYFSF STLFFAHRRP SESFFTRVLV VVWCFVLLIL
TQSYTATLTS MLTVQELRPT VRHMDDLRKS GVNIGYQTGS FTFERLKQMR FDESRLKTYN
SPEEMRELFL HKSSNGGIDA AFDEVAYIKL FMAKYCSEYS IIEPTFKADG FGFAFPLGSP
LVSDISRQIL NITEGDAMKA IENKWFLGEK HCLDSTTSDS PIQLDHHSFE ALFLIVFVVS
VILLLLMLAS RGYQERQHNA SPNLPNDQAN AAQEEVNEEG NVGDHIVEVD TALVRRKKLT
SNTIPIRRVA PLSRLKSA
