GLR35_ARATH
ID GLR35_ARATH Reviewed; 953 AA.
AC Q9SW97; F4ITQ1; Q9ZV67;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 16-JAN-2004, sequence version 2.
DT 25-MAY-2022, entry version 136.
DE RecName: Full=Glutamate receptor 3.5;
DE AltName: Full=Ionotropic glutamate receptor GLR6;
DE AltName: Full=Ligand-gated ion channel 3.5;
DE Flags: Precursor;
GN Name=GLR3.5; Synonyms=GLR6; OrderedLocusNames=At2g32390; ORFNames=T32F6.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND NOMENCLATURE.
RC TISSUE=Seedling;
RX PubMed=11379626; DOI=10.1126/science.292.5521.1486b;
RA Lacombe B., Becker D., Hedrich R., DeSalle R., Hollmann M., Kwak J.M.,
RA Schroeder J.I., Le Novere N., Nam H.G., Spalding E.P., Tester M.,
RA Turano F.J., Chiu J., Coruzzi G.;
RT "The identity of plant glutamate receptors.";
RL Science 292:1486-1487(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=12082126; DOI=10.1093/oxfordjournals.molbev.a004165;
RA Chiu J.C., Brenner E.D., DeSalle R., Nitabach M.N., Holmes T.C.,
RA Coruzzi G.M.;
RT "Phylogenetic and expression analysis of the glutamate-receptor-like gene
RT family in Arabidopsis thaliana.";
RL Mol. Biol. Evol. 19:1066-1082(2002).
CC -!- FUNCTION: Glutamate-gated receptor that probably acts as non-selective
CC cation channel. May be involved in light-signal transduction and
CC calcium homeostasis via the regulation of calcium influx into cells.
CC -!- SUBUNIT: May form heteromers. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9SW97-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed predominantly in roots. Also detected in
CC shoots. {ECO:0000269|PubMed:12082126}.
CC -!- SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10.1)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC69939.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AEC08676.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF170494; AAD50976.1; -; mRNA.
DR EMBL; AC005700; AAC69939.2; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC08676.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; ANM61816.1; -; Genomic_DNA.
DR PIR; E84732; E84732.
DR PIR; T51134; T51134.
DR RefSeq; NP_001324013.1; NM_001336391.1. [Q9SW97-1]
DR RefSeq; NP_565743.1; NM_128798.1.
DR AlphaFoldDB; Q9SW97; -.
DR SMR; Q9SW97; -.
DR STRING; 3702.AT2G32390.3; -.
DR PRIDE; Q9SW97; -.
DR ProteomicsDB; 247365; -. [Q9SW97-1]
DR EnsemblPlants; AT2G32390.5; AT2G32390.5; AT2G32390. [Q9SW97-1]
DR GeneID; 817800; -.
DR Gramene; AT2G32390.5; AT2G32390.5; AT2G32390. [Q9SW97-1]
DR KEGG; ath:AT2G32390; -.
DR Araport; AT2G32390; -.
DR InParanoid; Q9SW97; -.
DR OMA; YHCNFRI; -.
DR OrthoDB; 188544at2759; -.
DR PhylomeDB; Q9SW97; -.
DR PRO; PR:Q9SW97; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SW97; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0005262; F:calcium channel activity; ISS:UniProtKB.
DR GO; GO:0008066; F:glutamate receptor activity; ISS:UniProtKB.
DR GO; GO:0015276; F:ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0038023; F:signaling receptor activity; IBA:GO_Central.
DR GO; GO:0006816; P:calcium ion transport; ISS:UniProtKB.
DR GO; GO:0019722; P:calcium-mediated signaling; ISS:UniProtKB.
DR GO; GO:0071230; P:cellular response to amino acid stimulus; ISS:UniProtKB.
DR CDD; cd19990; PBP1_GABAb_receptor_plant; 1.
DR InterPro; IPR001828; ANF_lig-bd_rcpt.
DR InterPro; IPR044440; GABAb_receptor_plant_PBP1.
DR InterPro; IPR001320; Iontro_rcpt.
DR InterPro; IPR017103; Iontropic_Glu_rcpt_pln.
DR InterPro; IPR028082; Peripla_BP_I.
DR InterPro; IPR001638; Solute-binding_3/MltF_N.
DR Pfam; PF01094; ANF_receptor; 1.
DR Pfam; PF00060; Lig_chan; 1.
DR Pfam; PF00497; SBP_bac_3; 1.
DR PIRSF; PIRSF037090; Iontro_Glu-like_rcpt_pln; 1.
DR SMART; SM00079; PBPe; 1.
DR SUPFAM; SSF53822; SSF53822; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Ion channel; Ion transport;
KW Ligand-gated ion channel; Membrane; Receptor; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..953
FT /note="Glutamate receptor 3.5"
FT /id="PRO_0000011609"
FT TOPO_DOM 30..606
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 607..627
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 628..636
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 637..657
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 658..668
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 669..689
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 690..850
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 851..871
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 872..953
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 928..953
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 937..953
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 38
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 95
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 223
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 371
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 397
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 436
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 454
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 569
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 78
FT /note="D -> G (in Ref. 1; AAD50976)"
FT /evidence="ECO:0000305"
FT CONFLICT 104..107
FT /note="GALQ -> E (in Ref. 1; AAD50976)"
FT /evidence="ECO:0000305"
FT CONFLICT 127
FT /note="I -> V (in Ref. 1; AAD50976)"
FT /evidence="ECO:0000305"
FT CONFLICT 159
FT /note="R -> C (in Ref. 1; AAD50976)"
FT /evidence="ECO:0000305"
FT CONFLICT 443
FT /note="A -> V (in Ref. 1; AAD50976)"
FT /evidence="ECO:0000305"
FT CONFLICT 459
FT /note="D -> N (in Ref. 1; AAD50976)"
FT /evidence="ECO:0000305"
FT CONFLICT 495
FT /note="V -> I (in Ref. 1; AAD50976)"
FT /evidence="ECO:0000305"
FT CONFLICT 550
FT /note="S -> G (in Ref. 1; AAD50976)"
FT /evidence="ECO:0000305"
FT CONFLICT 860
FT /note="V -> A (in Ref. 1; AAD50976)"
FT /evidence="ECO:0000305"
FT CONFLICT 878
FT /note="Q -> H (in Ref. 1; AAD50976)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 953 AA; 106811 MW; 9C13F10ECFFF5116 CRC64;
MGFFVMIRDV SMGFMLLCIS ALWVLPIQGA GRESFSRNSS SSSLPSSVNV GALFTYDSFI
GRAAKLAFVA AIEDINADQS ILRGTKLNIV FQDTNCSGFV GTMGALQLME NKVVAAIGPQ
SSGIGHIISH VANELHVPFL SFAATDPTLS SLQYPYFLRT TQNDYFQMNA ITDFVSYFRW
REVVAIFVDD EYGRNGISVL GDALAKKRAK ISYKAAFPPG ADNSSISDLL ASVNLMESRI
FVVHVNPDSG LNIFSVAKSL GMMGSGYVWI TTDWLLTALD SMEPLDPRAL DLLQGVVAFR
HYTPESDNKR QFKGRWKNLR FKESLKSDDG FNSYALYAYD SVWLVARALD VFFSQGNTVT
FSNDPSLRNT NDSGIKLSKL HIFNEGERFL QVILEMNYTG LTGQIEFNSE KNRINPAYDI
LNIKSTGPLR VGYWSNHTGF SVAPPETLYS KPSNTSAKDQ RLNEIIWPGE VIKPPRGWVF
PENGKPLKIG VPNRVSYKNY ASKDKNPLGV KGFCIDIFEA AIQLLPYPVP RTYILYGDGK
KNPSYDNLIS EVAANIFDVA VGDVTIITNR TKFVDFTQPF IESGLVVVAP VKGAKSSPWS
FLKPFTIEMW AVTGALFLFV GAVIWILEHR FNEEFRGPPR RQIITVFWFS FSTMFFSHRE
NTVSTLGRFV LLVWLFVVLI INSSYTASLT SILTVQQLTS RIEGMDTLIA SNEPIGVQDG
TFAWKFLVNE LNIAPSRIIP LKDEEEYLSA LQRGPRGGGV AAIVDELPYI KALLSNSNCK
FRTVGQEFTR TGWGFAFQRD SPLAVDMSTA ILQLAEEGKL EKIRKKWLTY DHECTMQISD
TENYQISVQS FWGLFLICGV VWFIALTLFC WKVFWQYQRL RPEESDEVQA RSEEAGSSRG
KSLRAVSFKD LIKVVDKREA EIKEMLKEKS SKKLKDGQSS AENSQSKDHE TPQ
