ID   AMIB_PSEAE              Reviewed;         371 AA.
AC   Q51416;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   08-DEC-2000, sequence version 2.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=ATP-dependent protease ATP-binding subunit-like protein AmiB;
GN   Name=amiB; OrderedLocusNames=PA3365;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PAC1;
RX   PubMed=7642533; DOI=10.1074/jbc.270.32.18818;
RA   Wilson S.A., Williams R.J., Pearl L.H., Drew R.E.;
RT   "Identification of two new genes in the Pseudomonas aeruginosa amidase
RT   operon, encoding an ATPase (AmiB) and a putative integral membrane protein
RT   (AmiS).";
RL   J. Biol. Chem. 270:18818-18824(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-13.
RC   STRAIN=PAC1;
RX   PubMed=7539417; DOI=10.1128/jb.177.11.3052-3057.1995;
RA   Wilson S.A., Drew R.E.;
RT   "Transcriptional analysis of the amidase operon from Pseudomonas
RT   aeruginosa.";
RL   J. Bacteriol. 177:3052-3057(1995).
CC   -!- FUNCTION: Unlikely to encode a regulatory protein. Has ATPase activity.
CC       AmiB and AmiS may act jointly into a two component ABC transporter
CC       system.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC   -!- SIMILARITY: Belongs to the ClpX chaperone family. {ECO:0000305}.
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DR   EMBL; X77160; CAA54405.1; -; Genomic_DNA.
DR   EMBL; AE004091; AAG06753.1; -; Genomic_DNA.
DR   PIR; D83226; D83226.
DR   PIR; S44145; S44145.
DR   RefSeq; NP_252055.1; NC_002516.2.
DR   RefSeq; WP_003113130.1; NZ_QZGE01000017.1.
DR   AlphaFoldDB; Q51416; -.
DR   SMR; Q51416; -.
DR   STRING; 287.DR97_4562; -.
DR   PaxDb; Q51416; -.
DR   PRIDE; Q51416; -.
DR   EnsemblBacteria; AAG06753; AAG06753; PA3365.
DR   GeneID; 877799; -.
DR   KEGG; pae:PA3365; -.
DR   PATRIC; fig|208964.12.peg.3524; -.
DR   PseudoCAP; PA3365; -.
DR   HOGENOM; CLU_005070_9_1_6; -.
DR   InParanoid; Q51416; -.
DR   OMA; FCRVDMN; -.
DR   PhylomeDB; Q51416; -.
DR   BioCyc; PAER208964:G1FZ6-3429-MON; -.
DR   SABIO-RK; Q51416; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR   GO; GO:0034605; P:cellular response to heat; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003959; ATPase_AAA_core.
DR   InterPro; IPR019489; Clp_ATPase_C.
DR   InterPro; IPR001270; ClpA/B.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF07724; AAA_2; 1.
DR   Pfam; PF10431; ClpB_D2-small; 1.
DR   PRINTS; PR00300; CLPPROTEASEA.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM01086; ClpB_D2-small; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..371
FT                   /note="ATP-dependent protease ATP-binding subunit-like
FT                   protein AmiB"
FT                   /id="PRO_0000160568"
FT   BINDING         96..103
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        43
FT                   /note="R -> H (in Ref. 1; CAA54405)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        65
FT                   /note="D -> E (in Ref. 1; CAA54405)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        356
FT                   /note="T -> I (in Ref. 1; CAA54405)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   371 AA;  42130 MW;  DFC1C608DA6EEBCF CRC64;
     MPFLSDMLDQ SRRQQDEEQA LARENLAEAS LLQAHLSHRS ALRSRFRFDP AAVMDCLRAE
     VLGQDPALQA VEDMLKVVRA DIADPRRPLF SALFLGPTGV GKTEIVRALA RALHGDAEGF
     CRVDMNTLSQ EHYAAALTGA PPGYVGAKEG TTLLEQDKLD GSPGRPGIVL FDELEKASPE
     VVHALLNVLD NGLLRVASGE RTYHFRNTLV FMTSNLCAHE IQRYDERRQR LPWRLLPVGG
     ERRRRDIDGM VRARLLKTFS PEFVNRLDSV VTFNWIERDV VARLVELEVQ RLNRRLEKHR
     CRLEATPEVL AKIARAGFDR QFGARALRRS VRHHLEVPLA EHLLDHHQPG DGNCTTYLAS
     LEHERVRFVR R