ID   AMID_PSECL              Reviewed;         506 AA.
AC   P27765;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Amidase;
DE            EC=3.5.1.4;
OS   Pseudomonas chlororaphis (Pseudomonas aureofaciens).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-35.
RC   STRAIN=B23;
RX   PubMed=2013568; DOI=10.1128/jb.173.8.2465-2472.1991;
RA   Nishiyama M., Horinouchi S., Kobayashi M., Nagasawa T., Yamada H.,
RA   Beppu T.;
RT   "Cloning and characterization of genes responsible for metabolism of
RT   nitrile compounds from Pseudomonas chlororaphis B23.";
RL   J. Bacteriol. 173:2465-2472(1991).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a monocarboxylic acid amide + H2O = a monocarboxylate +
CC         NH4(+); Xref=Rhea:RHEA:12020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:35757, ChEBI:CHEBI:83628; EC=3.5.1.4;
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
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DR   EMBL; D90216; BAA14244.1; -; Genomic_DNA.
DR   PIR; C42725; C42725.
DR   AlphaFoldDB; P27765; -.
DR   SMR; P27765; -.
DR   BioCyc; MetaCyc:MON-15571; -.
DR   GO; GO:0004040; F:amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0043864; F:indoleacetamide hydrolase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR000120; Amidase.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR11895; PTHR11895; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:2013568"
FT   CHAIN           2..506
FT                   /note="Amidase"
FT                   /id="PRO_0000105124"
FT   ACT_SITE        98
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        173
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        197
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   506 AA;  54178 MW;  60C6C22B24DCBC35 CRC64;
     MAITRPTLDQ VLDIRTQLHM QLTHEQAASY LELMQPSFDA YDLVDELADF VPPIRYDRSS
     GYRHRPSAKE NPLNAWYYRT EVNGAREGLL AGKTVALKDN ISLAGVPMMN GAAPLEGFVP
     GFDATVVTRL LDAGATILGK ATCEHYCLSG GSHTSDPAPV HNPHRHGYAS GGSSSGSAAL
     VASGEVDIAV GGDQGGSIRI PSAFCGTYGM KPTHGLVPYT GVMAIEATID HVGPITGNVR
     DNALMLQAMA GADGLDPRQA APQVDDYCSY LEKGVSGLRI GVLQEGFALA NQDPRVADKV
     RDAIARLEAL GAHVEPVSIP EHNLAGLLWH PIGCEGLTMQ MMHGNGAGFN WKGLYDVGLL
     DKQASWRDDA DQLSASLKLC MFVGQYGLSR YNGRYYAKAQ NLARFARQGY DKALQTYDLL
     VMPTTPITAQ PHPPANCSIT EYVARALEMI GNTAPQDITG HPAMSIPCGL LDGLPVGLML
     VAKHYAEGTI YQAAAAFEAS VDWRTL