ID   AMID_PSEPU              Reviewed;         466 AA.
AC   O69768;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Amidase;
DE            EC=3.5.1.4;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRRL 18668;
RX   PubMed=9809753; DOI=10.1089/dna.1998.17.915;
RA   Wu S., Fallon R.D., Payne M.S.;
RT   "Cloning and nucleotide sequence of amidase gene from Pseudomonas putida.";
RL   DNA Cell Biol. 17:915-920(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a monocarboxylic acid amide + H2O = a monocarboxylate +
CC         NH4(+); Xref=Rhea:RHEA:12020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:35757, ChEBI:CHEBI:83628; EC=3.5.1.4;
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
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DR   EMBL; U89363; AAC18422.1; -; Genomic_DNA.
DR   AlphaFoldDB; O69768; -.
DR   SMR; O69768; -.
DR   GO; GO:0004040; F:amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0043864; F:indoleacetamide hydrolase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR000120; Amidase.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR11895; PTHR11895; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   3: Inferred from homology;
KW   Hydrolase.
FT   CHAIN           1..466
FT                   /note="Amidase"
FT                   /id="PRO_0000105125"
FT   REGION          128..152
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        79
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        148
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        172
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   466 AA;  50162 MW;  AE9FF610B7CF4CF5 CRC64;
     MSSLTRLTLA QVAQKLKARE VSAVEVLDAC LTQVRSTEKQ ISAYVCVLED QARAAAHATD
     ADIRGRWKGP LHGVPVAVKD LYDIAGVPTT ASSPAHELDA QQDPARVRRL QDAGAVILGK
     THTHEFAYGR ITPKSRNPRD PGRTPGGSSG GSAATVAACC VYLATGTDTG GSVRIPSSMC
     NTVGLKQPTV GRVHGAGVSS LSWSLDHPGP ITRTVEDTAL MLQVMAGFDP ADPRSLDEPV
     PSYAEGLGQG VKGLRWGVPK NYFFDRVDPE VESAVRAAID QLKELGAELV EVEVPMAEQI
     IPVKFGIMLP EASAYHRTML RESPELYTAD VRILLELGDL VTATDYLQAQ RVRTLMQRAV
     AEMYQRIDVL IAPTLPIPAA RSGEEVHTWP DGTVEALVMA YTRFTSFGNV TGLPTLNLPC
     GFSKDGLRSA CRSGRPLDEK TLLRAGLAYE KATTWHQRHP ELIGAG