GLS1_CAEEL
ID GLS1_CAEEL Reviewed; 1052 AA.
AC Q8I4M5; C3UPA2; C6KRM2; Q93342;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 2.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Germline survival defective-1 {ECO:0000312|EMBL:ACO95124.1};
DE Flags: Precursor;
GN Name=gls-1 {ECO:0000312|EMBL:ACO95124.1, ECO:0000312|WormBase:C36B1.8b};
GN ORFNames=C36B1.8;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000305, ECO:0000312|EMBL:ACO95124.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C), ALTERNATIVE SPLICING, FUNCTION,
RP INTERACTION WITH GLD-3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=19461891; DOI=10.1371/journal.pgen.1000494;
RA Rybarska A., Harterink M., Jedamzik B., Kupinski A.P., Schmid M.,
RA Eckmann C.R.;
RT "GLS-1, a novel P granule component, modulates a network of conserved RNA
RT regulators to influence germ cell fate decisions.";
RL PLoS Genet. 5:E1000494-E1000494(2009).
RN [2] {ECO:0000312|EMBL:CAD56562.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305, ECO:0000312|EMBL:ACO95124.1}
RP FUNCTION, AND INTERACTION WITH GLD-4.
RX PubMed=19339688; DOI=10.1101/gad.494009;
RA Schmid M., Kuchler B., Eckmann C.R.;
RT "Two conserved regulatory cytoplasmic poly(A) polymerases, GLD-4 and GLD-2,
RT regulate meiotic progression in C. elegans.";
RL Genes Dev. 23:824-836(2009).
CC -!- FUNCTION: Required maternally for germline survival by forming a
CC maternal complex with gld-3. During hermaphrodite development forms a
CC complex with gld-3 which promotes the sperm/oocyte switch freeing the
CC translational repressor fbf to turn off sperm promoting factors.
CC Required for proper oocyte differentiation and oogenic meiotic arrest.
CC Stimulates the enzymatic activity of gld-4 and together they prevent
CC gld-1 mRNA degradation. {ECO:0000269|PubMed:19339688,
CC ECO:0000269|PubMed:19461891}.
CC -!- SUBUNIT: Isoform C interacts (via C-terminus) with gld-3 isoform A (via
CC C-terminus) in an RNA-independent manner. Isoform C interacts with gld-
CC 4. {ECO:0000269|PubMed:19339688, ECO:0000269|PubMed:19461891}.
CC -!- INTERACTION:
CC Q8I4M5; Q95ZK7-1: gld-3; NbExp=3; IntAct=EBI-322416, EBI-14989519;
CC Q8I4M5-3; Q95ZK7-1: gld-3; NbExp=6; IntAct=EBI-14989623, EBI-14989519;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19461891}.
CC Cytoplasmic granule {ECO:0000269|PubMed:19461891}. Note=Localizes to P
CC granules. {ECO:0000269|PubMed:19461891}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Comment=Additional isoforms seem to exist. Experimental confirmation
CC may be lacking for some isoforms. {ECO:0000305};
CC Name=b {ECO:0000269|PubMed:9851916};
CC IsoId=Q8I4M5-1; Sequence=Displayed;
CC Name=a {ECO:0000269|PubMed:9851916};
CC IsoId=Q8I4M5-2; Sequence=VSP_043968;
CC Name=c {ECO:0000269|PubMed:19461891, ECO:0000269|PubMed:9851916};
CC IsoId=Q8I4M5-3; Sequence=VSP_043967;
CC -!- TISSUE SPECIFICITY: Expressed in the germline (at protein level). In
CC the early embryo is expressed in all cells, then becomes gradually
CC restricted to the germ cell lineage and enriches in P granules (at
CC protein level). In adult hermaphrodites, is expressed in the mitotic
CC region, accumulates during early stages of meiotic prophase I and is
CC slightly less abundant in maturing oocytes (at protein level).
CC {ECO:0000269|PubMed:19461891}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically
CC throughout development and in adult hermaphrodites (at protein level).
CC {ECO:0000269|PubMed:19461891}.
CC -!- DISRUPTION PHENOTYPE: Loss of maternal and zygotic expression causes
CC germline survival defects. Zygotic mutants show a masculinized
CC hermaphrodite germline. At 25 degress Celsius, hermaphrodite zygotic
CC mutants show small oogenic cells that are either arrested in pachytene
CC or have undergone abnormal meiotic prophase progression and failed to
CC arrest in diakinesis, often resulting in endoreduplicating oocyte
CC nuclei. {ECO:0000269|PubMed:19461891}.
CC -!- MISCELLANEOUS: Trans-spliced exclusively to SL2, suggesting a
CC transcriptional coregulation with the upstream gene dhfr-1.
CC {ECO:0000269|PubMed:19461891}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACO95124.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; FJ610055; ACO95124.1; ALT_INIT; mRNA.
DR EMBL; Z80215; CAB02273.3; -; Genomic_DNA.
DR EMBL; Z80215; CAD56562.2; -; Genomic_DNA.
DR EMBL; Z80215; CAZ65476.1; -; Genomic_DNA.
DR PIR; T19779; T19779.
DR RefSeq; NP_001250878.1; NM_001263949.1. [Q8I4M5-3]
DR RefSeq; NP_492365.3; NM_059964.3. [Q8I4M5-2]
DR RefSeq; NP_871882.2; NM_182082.5. [Q8I4M5-1]
DR AlphaFoldDB; Q8I4M5; -.
DR BioGRID; 38115; 12.
DR ComplexPortal; CPX-1132; gls-1-gld-3 complex.
DR DIP; DIP-25830N; -.
DR IntAct; Q8I4M5; 6.
DR STRING; 6239.C36B1.8b; -.
DR EPD; Q8I4M5; -.
DR PaxDb; Q8I4M5; -.
DR PeptideAtlas; Q8I4M5; -.
DR EnsemblMetazoa; C36B1.8a.1; C36B1.8a.1; WBGene00007975. [Q8I4M5-2]
DR EnsemblMetazoa; C36B1.8b.1; C36B1.8b.1; WBGene00007975. [Q8I4M5-1]
DR EnsemblMetazoa; C36B1.8c.1; C36B1.8c.1; WBGene00007975. [Q8I4M5-3]
DR GeneID; 172682; -.
DR KEGG; cel:CELE_C36B1.8; -.
DR UCSC; C36B1.8b; c. elegans.
DR CTD; 172682; -.
DR WormBase; C36B1.8a; CE34472; WBGene00007975; gls-1. [Q8I4M5-2]
DR WormBase; C36B1.8b; CE34473; WBGene00007975; gls-1. [Q8I4M5-1]
DR WormBase; C36B1.8c; CE43936; WBGene00007975; gls-1. [Q8I4M5-3]
DR eggNOG; ENOG502TI2R; Eukaryota.
DR GeneTree; ENSGT00520000055637; -.
DR HOGENOM; CLU_013517_0_0_1; -.
DR InParanoid; Q8I4M5; -.
DR OMA; CEEFNGT; -.
DR OrthoDB; 933081at2759; -.
DR SignaLink; Q8I4M5; -.
DR PRO; PR:Q8I4M5; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00007975; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0043186; C:P granule; IDA:WormBase.
DR GO; GO:0005844; C:polysome; IDA:WormBase.
DR GO; GO:0018992; P:germ-line sex determination; IMP:ComplexPortal.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:ComplexPortal.
DR GO; GO:0006417; P:regulation of translation; IC:ComplexPortal.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Developmental protein; Meiosis;
KW Reference proteome; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..1052
FT /note="Germline survival defective-1"
FT /evidence="ECO:0000255"
FT /id="PRO_0000418088"
FT REGION 41..320
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 424..732
FT /note="gld-4 binding"
FT /evidence="ECO:0000269|PubMed:19339688"
FT REGION 478..543
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 667..689
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 892..1052
FT /note="gld-3 binding"
FT /evidence="ECO:0000269|PubMed:19461891"
FT REGION 933..965
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1033..1052
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 56..149
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 161..177
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 178..192
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 193..214
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 225..282
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 289..315
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 478..536
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 933..949
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 950..965
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..44
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000303|PubMed:19461891,
FT ECO:0000303|PubMed:9851916"
FT /id="VSP_043967"
FT VAR_SEQ 854..856
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000303|PubMed:9851916"
FT /id="VSP_043968"
SQ SEQUENCE 1052 AA; 116014 MW; 56C1934E11A5BE6A CRC64;
MRCLISYLFH SFLIFLKFIR SDVTALTLQE KRKKSRLTGI LMKSMANKKN HQQKKSTDGS
TMNGNNATAT AAATTQNSSQ IGQNSNSSHS VTNDNTSSNN ASTSTSSITS TTTTTTTVPS
SQQSQSQNQY SHQRLSSTSS TSQQTGISKF PAKKAGHNEL EKTSNTANSQ SGAFKGTTNK
DRPKEKEKNT HSGNRQSQVN HNHHHSNHVS GNQQNRKNKN RDHSNQSYRG GYTHNHNNYH
SLENAKSSGF LSNSSLSSAG QISASSAPPV STTPTAIPIF QESKIVQTDP PVEEAKKKKE
KPKIKRDEEP MPYKSTDPKN MDSVMAFKEH DEWDKVEFMC ELVSFLSPTD LRLLGNCIEG
SVRCYNNQMR PVEKTSNCSD PTAGLPQFVC SPPPPPQQVY YFPGIADAAL YQTRNAVNSV
FVQQHPPGLP PLLGGMQNIM YPPDANFHHS TSCTTPSSSV SVANKDVNNV PVVLSSSVNG
ISNNIPSDRQ QLDSKPNTAR GSSGNINQSN TTSPEPEPGS NHISTTAANP AHPTSVTVPQ
SVPPVPQEPE LFLKSVLDLT SYIYTLMAVC SSTNRKSAAK ISDYVQNVIL REKSQILERI
PDELDKITVL QEIGKIVAAM THHPAITLDD KMKYAALRDG LRAQIETLFR QYYTTQKQLE
QSNLVVPSSQ AVGDENDTDS DHESEEEFEP LSGVMGSRFE SNPVQPSPSV PGTFFIIRFI
GRQIEKNDNL FSLEIHWSDG DRTFAQRSRD QLKALQHRLL DEFGQQRSEK YLHQGCTTSY
SSFDDDNKKL SASTSTMETF APNGERIVPR LARDATPAQY VQYINELSDL PARMMLSAVI
CEEFNGTRAK TEDLLQETRE ASDGLIYSRW KNPRAKSPVR YFKRNATGSI DPIELPVNMQ
PFLYSNIPQT QVQTLFPSCS NCGGPHAPKH CEKQTLLSKK GDHRTRSDGE GSQQNGGTSS
SNSYAPIHAN LPHAVYIENP QAMLGSNHFN HHQQQHIIQN TIFHNGGQFR ANGTYEPQIP
IAYYHAQGTV QNSNNTGGVN GNSGGGNQNS NF
