ID   AMID_RHORH              Reviewed;         517 AA.
AC   P84650;
DT   27-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=Enantioselective amidase;
DE            EC=3.5.1.4;
GN   Name=amdA;
OS   Rhodococcus rhodochrous.
OC   Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Rhodococcus.
OX   NCBI_TaxID=1829;
RN   [1]
RP   PROTEIN SEQUENCE, CATALYTIC ACTIVITY, SUBUNIT, AND INDUCTION.
RC   STRAIN=M8 {ECO:0000269|Ref.1};
RA   Pertsovich S.I., Guranda D.T., Svedas V.K.;
RT   "New amidase from Rhodococcus rhodochrous possesses unusual substrate
RT   specificity.";
RL   Submitted (SEP-2005) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a monocarboxylic acid amide + H2O = a monocarboxylate +
CC         NH4(+); Xref=Rhea:RHEA:12020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:35757, ChEBI:CHEBI:83628; EC=3.5.1.4;
CC         Evidence={ECO:0000269|Ref.1};
CC   -!- SUBUNIT: Homooctamer. {ECO:0000269|Ref.1}.
CC   -!- INDUCTION: By aromatic and aliphatic amides. {ECO:0000269|Ref.1}.
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000255}.
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DR   AlphaFoldDB; P84650; -.
DR   SMR; P84650; -.
DR   GO; GO:0004040; F:amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0043864; F:indoleacetamide hydrolase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR000120; Amidase.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR11895; PTHR11895; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase.
FT   CHAIN           1..517
FT                   /note="Enantioselective amidase"
FT                   /id="PRO_0000105127"
FT   ACT_SITE        96
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:P97612"
FT   ACT_SITE        173
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:P97612"
FT   ACT_SITE        197
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:P97612"
SQ   SEQUENCE   517 AA;  54735 MW;  10F75F7AF1AA40F5 CRC64;
     MSSLTPPNSN QMSALNNHFF FGLTTPKLEE FAPALEATLA YAVTDERVYE RTAPEPPDRS
     WTTPTAAENP LSAWYVTTSI SYTDGGPLAG RTVAIKDNVT VAGVPMMNGS RVVEGFTPRY
     DATVLRRLLD AGATKAGKAV CEDLCFSGSS VTSHPQPVRN PWDESHGYKA GGSSSGSEAL
     VASGHVDCAV GGDGGGSIRI PLACCGIVGC KPTHGLKPYT FPIERTIDHL GPMTRTVGDA
     AMMLTVLAGT DGLDPRQADH RIEPVDYLAA LAEPAGLRVV VVTEGFDTPV QDAAVDDAVR
     AAILVLRSGC LTVEIVSIPI HLDAFAVWNV IATEGAAYQM LDGNYYGMNT GGFYDPELII
     HFSRRRLEHG HQLSKTVKLV GMGGRYTSET GGGKYAAAAR QLVREVRAAY DLALARYDVL
     VMPTLPYTAT KIPITDIPLA DYLDTALSMI INTAPFDVTG HPALCPVAGA VHGLPVGMMI
     IGKAHDDDAT VLRVAAFEHA VGNYPVPPEA ASTLATL