ID   AMID_SACS2              Reviewed;         504 AA.
AC   P95896;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   25-MAY-2022, entry version 113.
DE   RecName: Full=Amidase;
DE            EC=3.5.1.4;
GN   OrderedLocusNames=SSO2122; ORFNames=C02016, C02_017;
OS   Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS   (Sulfolobus solfataricus).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Saccharolobus.
OX   NCBI_TaxID=273057;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC   STRAIN=DSM 5833 / MT-4;
RX   PubMed=11453462; DOI=10.1007/s007920100190;
RA   d'Abusco A.S., Ammendola S., Scandurra R., Politi L.;
RT   "Molecular and biochemical characterization of the recombinant amidase from
RT   hyperthermophilic archaeon Sulfolobus solfataricus.";
RL   Extremophiles 5:183-192(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX   PubMed=8899719; DOI=10.1111/j.1365-2958.1996.tb02666.x;
RA   Sensen C.W., Klenk H.-P., Singh R.K., Allard G., Chan C.C.-Y., Liu Q.Y.,
RA   Penny S.L., Young F., Schenk M.E., Gaasterland T., Doolittle W.F.,
RA   Ragan M.A., Charlebois R.L.;
RT   "Organizational characteristics and information content of an archaeal
RT   genome: 156 kb of sequence from Sulfolobus solfataricus P2.";
RL   Mol. Microbiol. 22:175-191(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX   PubMed=11427726; DOI=10.1073/pnas.141222098;
RA   She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA   Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA   Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA   Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA   Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA   Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT   "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
CC   -!- FUNCTION: Enantioselective, active on 2- to 6-carbon aliphatic amides
CC       and on many aromatic amides.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a monocarboxylic acid amide + H2O = a monocarboxylate +
CC         NH4(+); Xref=Rhea:RHEA:12020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:35757, ChEBI:CHEBI:83628; EC=3.5.1.4;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Active from pH 4 to 9.;
CC       Temperature dependence:
CC         Active from 60 to 95 degrees Celsius.;
CC   -!- SUBUNIT: Monomer.
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
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DR   EMBL; AF290611; AAK83092.1; -; Genomic_DNA.
DR   EMBL; Y08256; CAA69448.1; -; Genomic_DNA.
DR   EMBL; AE006641; AAK42301.1; -; Genomic_DNA.
DR   PIR; S74034; S74034.
DR   RefSeq; WP_009990155.1; NC_002754.1.
DR   AlphaFoldDB; P95896; -.
DR   SMR; P95896; -.
DR   STRING; 273057.SSO2122; -.
DR   EnsemblBacteria; AAK42301; AAK42301; SSO2122.
DR   GeneID; 44130832; -.
DR   KEGG; sso:SSO2122; -.
DR   PATRIC; fig|273057.12.peg.2212; -.
DR   eggNOG; arCOG01717; Archaea.
DR   HOGENOM; CLU_009600_18_1_2; -.
DR   InParanoid; P95896; -.
DR   OMA; TTPEFGW; -.
DR   PhylomeDB; P95896; -.
DR   BRENDA; 3.5.1.4; 6163.
DR   BRENDA; 3.5.2.B2; 6163.
DR   BRENDA; 3.5.5.1; 6163.
DR   Proteomes; UP000001974; Chromosome.
DR   GO; GO:0004040; F:amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0043864; F:indoleacetamide hydrolase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR000120; Amidase.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR11895; PTHR11895; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..504
FT                   /note="Amidase"
FT                   /id="PRO_0000105263"
FT   ACT_SITE        96
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        171
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        195
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   504 AA;  55655 MW;  A9103AB7D09A88EE CRC64;
     MGIKLPTLED LREISKQFNL DLEDEELKSF LQLLKLQLES YERLDSLPDY TPRVKYQRTL
     GRPPMKEENP YGALVWITSI KGKEEGKLKG KRICIKDNVM IAGIPMLNGS KMLEGFVPHM
     DATVVSRILD EAGEIVAKTT CEDLCFSGGS HTSYPWPVLN PRNPEYMAGG SSSGSAVAVA
     SGYCDMAVGG DQGGSIRIPS SWVGIYGLKP THGLVPYTGA FSIEPTLDHL GPMANTVKDV
     ALLLEVIAGR DELDSRQPDS LPPPPVKPYS KLIDGDVKDM KVGIVKEGFN WSNSEKDVDE
     LVLDSAKKLE DYGIKVEDTS IPLHRMGLDI WTPIAIEGAT ATMILGSGVG WGRKGLFETQ
     IADFFGNSLK SRARDLPNTV KGVLMLGYLM IKMYNNRYYA KARNLSIVLK EAYDSALRKY
     DALIMPTTPM KAMRYKSEPG FDEYFIMALG MINNTAPFDV TGHPAMNIPV GYSNGLPVGL
     MIIGRHFEED KVLKLANVFE RIKK