AMID_SYNY3
ID AMID_SYNY3 Reviewed; 506 AA.
AC Q55424;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=Putative amidase;
DE EC=3.5.1.4;
GN OrderedLocusNames=sll0828;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27184 / PCC 6803 / N-1;
RX PubMed=8590279; DOI=10.1093/dnares/2.4.153;
RA Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N.,
RA Sugiura M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region
RT from map positions 64% to 92% of the genome.";
RL DNA Res. 2:153-166(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a monocarboxylic acid amide + H2O = a monocarboxylate +
CC NH4(+); Xref=Rhea:RHEA:12020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:35757, ChEBI:CHEBI:83628; EC=3.5.1.4;
CC -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
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DR EMBL; BA000022; BAA10524.1; -; Genomic_DNA.
DR PIR; S75789; S75789.
DR AlphaFoldDB; Q55424; -.
DR SMR; Q55424; -.
DR IntAct; Q55424; 1.
DR STRING; 1148.1001278; -.
DR PaxDb; Q55424; -.
DR EnsemblBacteria; BAA10524; BAA10524; BAA10524.
DR KEGG; syn:sll0828; -.
DR eggNOG; COG0154; Bacteria.
DR InParanoid; Q55424; -.
DR OMA; FLTIWFA; -.
DR PhylomeDB; Q55424; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0004040; F:amidase activity; IEA:UniProtKB-EC.
DR GO; GO:0043864; F:indoleacetamide hydrolase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.90.1300.10; -; 1.
DR InterPro; IPR000120; Amidase.
DR InterPro; IPR020556; Amidase_CS.
DR InterPro; IPR023631; Amidase_dom.
DR InterPro; IPR036928; AS_sf.
DR PANTHER; PTHR11895; PTHR11895; 2.
DR Pfam; PF01425; Amidase; 1.
DR SUPFAM; SSF75304; SSF75304; 1.
DR PROSITE; PS00571; AMIDASES; 1.
PE 3: Inferred from homology;
KW Hydrolase; Reference proteome.
FT CHAIN 1..506
FT /note="Putative amidase"
FT /id="PRO_0000105262"
FT ACT_SITE 121
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 196
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 220
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 506 AA; 54254 MW; 11062B1FDB63B30A CRC64;
MRRQADRESV LPDYVKVLKP LPFNFSNFSN RQLFANFKMT DFCLLSALDL AQLVRTRQVS
PLEITQYYLD RLGKYDQTVG SFAHVAWESA IADAKQKTKY LAGMGNSEPL PPFFGVPIAV
KDLNCVADMP VSYGVSALKE NLATYDDGVV AKMKAAGFTI VGKTVTSQLG SFPYTEPPGF
LPARNPWHLD YNAGGSSGGS AAAVAAGLVP IAQGSDGGGS VRTPAACCSL VGFKPSRGRV
SQAPVGDYQS GIACHGPLSR TVLEAAALLD VMEGYITGDP YWLPSPDRPF VETTGETPGQ
LRLAYAFSLP PFSSSSEIQG AVAKVIAVCE NLGHQLEEAC FDVTSLIEPF AQIWKAGVGA
SGIPLPLLES VNQWLGETSG TAGDYLRGVR NMQVISRQIV GFMEQYDALI LPVFNHRPPK
IGEWTHLSPP DVVQKIIEWI APCPPANAAG LPAIAIPVGF DDQGLPLSVQ IIGKPAADAL
VLALAHQMEQ QLQGNQPRQL PLQFMP
