GLSA_GEOKA
ID GLSA_GEOKA Reviewed; 308 AA.
AC Q5KY26;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Glutaminase {ECO:0000255|HAMAP-Rule:MF_00313};
DE EC=3.5.1.2 {ECO:0000255|HAMAP-Rule:MF_00313};
GN Name=glsA {ECO:0000255|HAMAP-Rule:MF_00313}; OrderedLocusNames=GK2125;
OS Geobacillus kaustophilus (strain HTA426).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus;
OC Geobacillus thermoleovorans group.
OX NCBI_TaxID=235909;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HTA426;
RX PubMed=15576355; DOI=10.1093/nar/gkh970;
RA Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H.,
RA Matsui S., Uchiyama I.;
RT "Thermoadaptation trait revealed by the genome sequence of thermophilic
RT Geobacillus kaustophilus.";
RL Nucleic Acids Res. 32:6292-6303(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + L-glutamine = L-glutamate + NH4(+);
CC Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=3.5.1.2;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00313};
CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00313}.
CC -!- SIMILARITY: Belongs to the glutaminase family. {ECO:0000255|HAMAP-
CC Rule:MF_00313}.
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DR EMBL; BA000043; BAD76410.1; -; Genomic_DNA.
DR RefSeq; WP_011231610.1; NC_006510.1.
DR PDB; 2PBY; X-ray; 2.07 A; A/B/C/D=1-308.
DR PDBsum; 2PBY; -.
DR AlphaFoldDB; Q5KY26; -.
DR SMR; Q5KY26; -.
DR STRING; 235909.GK2125; -.
DR EnsemblBacteria; BAD76410; BAD76410; GK2125.
DR KEGG; gka:GK2125; -.
DR PATRIC; fig|235909.7.peg.2278; -.
DR eggNOG; COG2066; Bacteria.
DR HOGENOM; CLU_027932_1_0_9; -.
DR OMA; RPRNPFI; -.
DR EvolutionaryTrace; Q5KY26; -.
DR Proteomes; UP000001172; Chromosome.
DR GO; GO:0004359; F:glutaminase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:InterPro.
DR Gene3D; 3.40.710.10; -; 1.
DR HAMAP; MF_00313; Glutaminase; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR015868; Glutaminase.
DR PANTHER; PTHR12544; PTHR12544; 1.
DR Pfam; PF04960; Glutaminase; 1.
DR SUPFAM; SSF56601; SSF56601; 1.
DR TIGRFAMs; TIGR03814; Gln_ase; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Reference proteome.
FT CHAIN 1..308
FT /note="Glutaminase"
FT /id="PRO_1000048336"
FT BINDING 65
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT BINDING 116
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT BINDING 161
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT BINDING 168
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT BINDING 192
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT BINDING 244
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT BINDING 262
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT HELIX 6..17
FT /evidence="ECO:0007829|PDB:2PBY"
FT HELIX 18..22
FT /evidence="ECO:0007829|PDB:2PBY"
FT HELIX 30..34
FT /evidence="ECO:0007829|PDB:2PBY"
FT STRAND 41..46
FT /evidence="ECO:0007829|PDB:2PBY"
FT TURN 47..49
FT /evidence="ECO:0007829|PDB:2PBY"
FT STRAND 50..56
FT /evidence="ECO:0007829|PDB:2PBY"
FT HELIX 66..87
FT /evidence="ECO:0007829|PDB:2PBY"
FT HELIX 116..123
FT /evidence="ECO:0007829|PDB:2PBY"
FT STRAND 128..130
FT /evidence="ECO:0007829|PDB:2PBY"
FT HELIX 131..146
FT /evidence="ECO:0007829|PDB:2PBY"
FT HELIX 155..164
FT /evidence="ECO:0007829|PDB:2PBY"
FT HELIX 166..177
FT /evidence="ECO:0007829|PDB:2PBY"
FT HELIX 185..196
FT /evidence="ECO:0007829|PDB:2PBY"
FT STRAND 198..200
FT /evidence="ECO:0007829|PDB:2PBY"
FT HELIX 202..213
FT /evidence="ECO:0007829|PDB:2PBY"
FT TURN 214..216
FT /evidence="ECO:0007829|PDB:2PBY"
FT TURN 219..221
FT /evidence="ECO:0007829|PDB:2PBY"
FT STRAND 224..226
FT /evidence="ECO:0007829|PDB:2PBY"
FT HELIX 228..240
FT /evidence="ECO:0007829|PDB:2PBY"
FT STRAND 241..243
FT /evidence="ECO:0007829|PDB:2PBY"
FT HELIX 247..253
FT /evidence="ECO:0007829|PDB:2PBY"
FT STRAND 258..260
FT /evidence="ECO:0007829|PDB:2PBY"
FT STRAND 264..270
FT /evidence="ECO:0007829|PDB:2PBY"
FT TURN 271..273
FT /evidence="ECO:0007829|PDB:2PBY"
FT STRAND 274..279
FT /evidence="ECO:0007829|PDB:2PBY"
FT STRAND 287..289
FT /evidence="ECO:0007829|PDB:2PBY"
FT HELIX 290..303
FT /evidence="ECO:0007829|PDB:2PBY"
SQ SEQUENCE 308 AA; 33773 MW; 9D8954EFFC212BB9 CRC64;
MLVYNQEELV RFVEEAKQYA RYGKVADYIP ALGKANPNEL SIAIYTPDDE VVSAGDVTVK
VTLQSISKII ALALVLIDRG EDEVFHKVGM EPTDYPFHSI AKLEEKPAKP LNPMINAGAL
VVTSMIQGGS VSERLERLLA FVRRLAGNER ISYSDEVARS EFETAFLNRS LCYFLKQHRI
IDEDVEELME LYTKQCAIEM TCIDLARIGL VLALDGRDPH SSEPLMPLDV ARICKTFMVT
CGMYNSSGEF AIKVGIPAKS GVSGGILAAV PGRCGIGVFG PALDDKGNSL TGVKLLERLS
KTYSLSIF
