ID   GLSA_PSEAE              Reviewed;         302 AA.
AC   Q9I387;
DT   24-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2001, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Glutaminase {ECO:0000255|HAMAP-Rule:MF_00313};
DE            EC=3.5.1.2 {ECO:0000255|HAMAP-Rule:MF_00313};
GN   Name=glsA {ECO:0000255|HAMAP-Rule:MF_00313}; OrderedLocusNames=PA1638;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-glutamine = L-glutamate + NH4(+);
CC         Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=3.5.1.2;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00313};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00313}.
CC   -!- SIMILARITY: Belongs to the glutaminase family. {ECO:0000255|HAMAP-
CC       Rule:MF_00313}.
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DR   EMBL; AE004091; AAG05027.1; -; Genomic_DNA.
DR   PIR; E83441; E83441.
DR   RefSeq; NP_250329.1; NC_002516.2.
DR   RefSeq; WP_003114599.1; NZ_QZGE01000003.1.
DR   AlphaFoldDB; Q9I387; -.
DR   SMR; Q9I387; -.
DR   STRING; 287.DR97_251; -.
DR   PaxDb; Q9I387; -.
DR   PRIDE; Q9I387; -.
DR   EnsemblBacteria; AAG05027; AAG05027; PA1638.
DR   GeneID; 883013; -.
DR   KEGG; pae:PA1638; -.
DR   PATRIC; fig|208964.12.peg.1698; -.
DR   PseudoCAP; PA1638; -.
DR   HOGENOM; CLU_027932_1_1_6; -.
DR   InParanoid; Q9I387; -.
DR   OMA; RPRNPFI; -.
DR   PhylomeDB; Q9I387; -.
DR   BioCyc; PAER208964:G1FZ6-1668-MON; -.
DR   BRENDA; 3.5.1.2; 5087.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0004359; F:glutaminase activity; IBA:GO_Central.
DR   GO; GO:0006537; P:glutamate biosynthetic process; IBA:GO_Central.
DR   GO; GO:0006543; P:glutamine catabolic process; IBA:GO_Central.
DR   Gene3D; 3.40.710.10; -; 1.
DR   HAMAP; MF_00313; Glutaminase; 1.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR015868; Glutaminase.
DR   PANTHER; PTHR12544; PTHR12544; 1.
DR   Pfam; PF04960; Glutaminase; 1.
DR   SUPFAM; SSF56601; SSF56601; 1.
DR   TIGRFAMs; TIGR03814; Gln_ase; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..302
FT                   /note="Glutaminase"
FT                   /id="PRO_0000110616"
FT   BINDING         61
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT   BINDING         111
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT   BINDING         155
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT   BINDING         162
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT   BINDING         186
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT   BINDING         238
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT   BINDING         256
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
SQ   SEQUENCE   302 AA;  33046 MW;  12AE7C2383E3B552 CRC64;
     MQQLLNEILD EVRPLIGRGK VADYIPALAG VEPNQLGIAV YSRDGELFHA GDALRPFSIQ
     SISKVFSLVQ AIQHSGEDIW QRLGHEPSGQ PFNSLVQLEF ERGKPRNPFI NAGALVICDI
     NQSRFAAPAQ SMRDFVRRLC GNPEVVSDSV VARSEYQHRS RNAAAAYLMK SFGNFHNDVE
     AVLFSYFHHC ALRMSCVDLA RAFCFLADKG FCKHSGEQVL NERQTKQVNA IMATSGLYDE
     AGNFAYRVGL PGKSGVGGGI IAVVPGRFTV CVWSPELNAA GNSLAGIAAL EKLSERIGWS
     IF