GLSA_RALSO
ID GLSA_RALSO Reviewed; 304 AA.
AC Q8XQS6;
DT 24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Glutaminase {ECO:0000255|HAMAP-Rule:MF_00313};
DE EC=3.5.1.2 {ECO:0000255|HAMAP-Rule:MF_00313};
GN Name=glsA {ECO:0000255|HAMAP-Rule:MF_00313}; OrderedLocusNames=RSp1143;
GN ORFNames=RS05469;
OS Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum).
OG Plasmid megaplasmid Rsp.
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Ralstonia.
OX NCBI_TaxID=267608;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GMI1000;
RX PubMed=11823852; DOI=10.1038/415497a;
RA Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M.,
RA Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M.,
RA Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M.,
RA Moisan A., Robert C., Saurin W., Schiex T., Siguier P., Thebault P.,
RA Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A.;
RT "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL Nature 415:497-502(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + L-glutamine = L-glutamate + NH4(+);
CC Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=3.5.1.2;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00313};
CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00313}.
CC -!- SIMILARITY: Belongs to the glutaminase family. {ECO:0000255|HAMAP-
CC Rule:MF_00313}.
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DR EMBL; AL646053; CAD18294.1; -; Genomic_DNA.
DR RefSeq; WP_011004432.1; NC_003296.1.
DR AlphaFoldDB; Q8XQS6; -.
DR SMR; Q8XQS6; -.
DR STRING; 267608.RSp1143; -.
DR PRIDE; Q8XQS6; -.
DR EnsemblBacteria; CAD18294; CAD18294; RSp1143.
DR GeneID; 60504053; -.
DR KEGG; rso:RSp1143; -.
DR eggNOG; COG2066; Bacteria.
DR HOGENOM; CLU_027932_1_1_4; -.
DR OMA; RPRNPFI; -.
DR Proteomes; UP000001436; Plasmid megaplasmid Rsp.
DR GO; GO:0004359; F:glutaminase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:InterPro.
DR Gene3D; 3.40.710.10; -; 1.
DR HAMAP; MF_00313; Glutaminase; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR015868; Glutaminase.
DR PANTHER; PTHR12544; PTHR12544; 1.
DR Pfam; PF04960; Glutaminase; 1.
DR SUPFAM; SSF56601; SSF56601; 1.
DR TIGRFAMs; TIGR03814; Gln_ase; 1.
PE 3: Inferred from homology;
KW Hydrolase; Plasmid; Reference proteome.
FT CHAIN 1..304
FT /note="Glutaminase"
FT /id="PRO_0000110618"
FT BINDING 63
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT BINDING 113
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT BINDING 157
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT BINDING 164
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT BINDING 188
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT BINDING 240
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
FT BINDING 258
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00313"
SQ SEQUENCE 304 AA; 33262 MW; 03B1D8BBF4AC88B9 CRC64;
MDYQAILETI HRDIQPWLGK GRVADYIPEL AKASATDFGM AIVTPRGEVF RVGQAETLFS
IQSISKLFAC TLAFQLEGES LWQRVGREPS GNAFNSLVQL EHENGIPRNP FINAGALVVT
DVLCRRFVQA ETAMVQFMRR LVDNPRVDYN PRVALSELEH ADRNRAMAHF MRSFGNLHMP
VETVIDAYCR QCAIEMHCVD LARAVLFLAN GGVVPWSGER VIETSPAKRL SALMLTCGTY
DAAGDFVYRV GLPAKSGVGG GIVAVLPGEF GVCVWSPGLD VSGNSLAGLQ ALEWLTTLSG
RSIF