ID   GLSA_RHIEC              Reviewed;         309 AA.
AC   O87405; Q2K7U5;
DT   24-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 2.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Thermolabile glutaminase;
DE            EC=3.5.1.2;
DE   AltName: Full=Glutaminase A;
GN   Name=glsA; OrderedLocusNames=RHE_CH02311;
OS   Rhizobium etli (strain CFN 42 / ATCC 51251).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=347834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10095071; DOI=10.1016/s0167-4781(99)00026-3;
RA   Calderon J., Huerta-Saquero A., Du Pont G., Duran S.;
RT   "Sequence and molecular analysis of the Rhizobium etli glsA gene, encoding
RT   a thermolabile glutaminase.";
RL   Biochim. Biophys. Acta 1444:451-456(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFN 42 / ATCC 51251;
RX   PubMed=16505379; DOI=10.1073/pnas.0508502103;
RA   Gonzalez V., Santamaria R.I., Bustos P., Hernandez-Gonzalez I.,
RA   Medrano-Soto A., Moreno-Hagelsieb G., Janga S.C., Ramirez M.A.,
RA   Jimenez-Jacinto V., Collado-Vides J., Davila G.;
RT   "The partitioned Rhizobium etli genome: genetic and metabolic redundancy in
RT   seven interacting replicons.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:3834-3839(2006).
RN   [3]
RP   CHARACTERIZATION.
RX   PubMed=9126674; DOI=10.1007/bf00570126;
RA   Duran S., Sanchez-Linares L., Huerta-Saquero A., Du Pont G.,
RA   Huerta-Zepeda A., Calderon J.;
RT   "Identification of two glutaminases in Rhizobium etli.";
RL   Biochem. Genet. 34:453-465(1996).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-glutamine = L-glutamate + NH4(+);
CC         Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=3.5.1.2;
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glutaminase family. {ECO:0000305}.
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DR   EMBL; AF057158; AAC63991.1; -; Genomic_DNA.
DR   EMBL; CP000133; ABC91091.1; -; Genomic_DNA.
DR   RefSeq; WP_011425571.1; NC_007761.1.
DR   AlphaFoldDB; O87405; -.
DR   SMR; O87405; -.
DR   STRING; 347834.RHE_CH02311; -.
DR   EnsemblBacteria; ABC91091; ABC91091; RHE_CH02311.
DR   GeneID; 61480721; -.
DR   KEGG; ret:RHE_CH02311; -.
DR   eggNOG; COG2066; Bacteria.
DR   HOGENOM; CLU_027932_1_1_5; -.
DR   OMA; RPRNPFI; -.
DR   Proteomes; UP000001936; Chromosome.
DR   GO; GO:0004359; F:glutaminase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:InterPro.
DR   Gene3D; 3.40.710.10; -; 1.
DR   HAMAP; MF_00313; Glutaminase; 1.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR015868; Glutaminase.
DR   PANTHER; PTHR12544; PTHR12544; 1.
DR   Pfam; PF04960; Glutaminase; 1.
DR   SUPFAM; SSF56601; SSF56601; 1.
DR   TIGRFAMs; TIGR03814; Gln_ase; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..309
FT                   /note="Thermolabile glutaminase"
FT                   /id="PRO_0000110619"
FT   BINDING         64
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         114
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         160
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         167
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         191
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         243
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         261
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        94
FT                   /note="A -> T (in Ref. 1; AAC63991)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   309 AA;  32987 MW;  CC678FB5E6A048FD CRC64;
     MADLQATLDS IYTDILPRIG EGKVADYIPE LAKIDPRQFG MAIVTVDGQV FRVGDADIAF
     SIQSISKVFM LTLALGKVGE GLWKRVGREP SGSAFNSIVQ LEHESGIPRN PFINAGAIAV
     TDVVMAGHAP REAIGELLRF VRYLADDESI TIDDKVARSE TQTGYRNVAL ANFMRAYRNL
     DHPVDHVLGV YFHQCALAMS CEQLARAGLF LAARGSNPMT GHSVVSPKRA RRINALMLTC
     GHYDGSGDFA YHVGLPGKSG VGGGIFAVAP GIASIAVWSP GLNKVGNSQL GAVALEMLAA
     RTGWSVFGD