ID   GLT1_ARATH              Reviewed;         220 AA.
AC   P92995; Q29PR9; Q9LMD0;
DT   29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   29-AUG-2001, sequence version 2.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=Germin-like protein subfamily T member 1;
DE   Flags: Precursor;
GN   Name=GLP1; Synonyms=GLP4; OrderedLocusNames=At1g18970;
GN   ORFNames=F14D16.12, F14D16_7;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 9-220.
RC   STRAIN=cv. Columbia;
RX   PubMed=9869400; DOI=10.1023/a:1006038117130;
RA   Carter C., Graham R.A., Thornburg R.W.;
RT   "Arabidopsis thaliana contains a large family of germin-like proteins:
RT   characterization of cDNA and genomic sequences encoding 12 unique family
RT   members.";
RL   Plant Mol. Biol. 38:929-943(1998).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 17-220.
RC   STRAIN=cv. Columbia;
RA   Kim C.J., Chen H., Shinn P., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May play a role in plant defense. Probably has no oxalate
CC       oxidase activity even if the active site is conserved.
CC   -!- SUBUNIT: Oligomer (believed to be a pentamer but probably hexamer).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the germin family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB51565.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AC068602; AAF79304.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE29785.1; -; Genomic_DNA.
DR   EMBL; U75187; AAB51565.1; ALT_INIT; mRNA.
DR   EMBL; BT024837; ABD60720.1; -; mRNA.
DR   RefSeq; NP_564067.2; NM_101754.3.
DR   AlphaFoldDB; P92995; -.
DR   SMR; P92995; -.
DR   STRING; 3702.AT1G18970.1; -.
DR   PaxDb; P92995; -.
DR   PRIDE; P92995; -.
DR   ProteomicsDB; 248429; -.
DR   EnsemblPlants; AT1G18970.1; AT1G18970.1; AT1G18970.
DR   GeneID; 838478; -.
DR   Gramene; AT1G18970.1; AT1G18970.1; AT1G18970.
DR   KEGG; ath:AT1G18970; -.
DR   Araport; AT1G18970; -.
DR   TAIR; locus:2011291; AT1G18970.
DR   eggNOG; ENOG502QSRM; Eukaryota.
DR   HOGENOM; CLU_015790_0_3_1; -.
DR   InParanoid; P92995; -.
DR   OMA; MITAFNS; -.
DR   OrthoDB; 1164277at2759; -.
DR   PhylomeDB; P92995; -.
DR   PRO; PR:P92995; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; P92995; baseline and differential.
DR   Genevisible; P92995; AT.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR   Gene3D; 2.60.120.10; -; 1.
DR   InterPro; IPR006045; Cupin_1.
DR   InterPro; IPR001929; Germin.
DR   InterPro; IPR019780; Germin_Mn-BS.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF00190; Cupin_1; 1.
DR   PRINTS; PR00325; GERMIN.
DR   SMART; SM00835; Cupin_1; 1.
DR   SUPFAM; SSF51182; SSF51182; 1.
DR   PROSITE; PS00725; GERMIN; 1.
PE   2: Evidence at transcript level;
KW   Apoplast; Disulfide bond; Glycoprotein; Manganese; Metal-binding;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..220
FT                   /note="Germin-like protein subfamily T member 1"
FT                   /id="PRO_0000010830"
FT   DOMAIN          64..212
FT                   /note="Cupin type-1"
FT                   /evidence="ECO:0000255"
FT   BINDING         112
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         114
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         119
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         158
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        136
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        19..37
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   220 AA;  23214 MW;  0F60BFFECBD24098 CRC64;
     MATLQIPSAL LRSFLLMFCL FVIPSLSSDS DPLQDFCVGD LKASASINGF PCKSAVSASD
     FFYSGLGGPL DTSNPNGVTV APANVLTFPG LNTLGISMNN VELAPGGVNP PHLHPRATEV
     GTVIEGSVFV GFLSTNNTLF SKVLNAGEAF VIPRGLVHFQ WNVGQVKARM ITAFNSQLPG
     AVVLPSTLFG SKPEIPNAVL TRAFRTDDTT VQNLKSKFAV