GLT2_ORYSJ
ID GLT2_ORYSJ Reviewed; 2188 AA.
AC Q0DG35; A7VKB1; Q6I618; Q6I619;
DT 13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT 13-JUL-2010, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Glutamate synthase 2 [NADH], chloroplastic;
DE EC=1.4.1.14;
DE AltName: Full=NADH-dependent glutamate synthase 2;
DE Short=NADH-GOGAT 2;
DE Flags: Precursor;
GN OrderedLocusNames=Os05g0555600, LOC_Os05g48200;
GN ORFNames=OJ1214_E03.1, OJ1263_E10.17;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Sasanishiki; TISSUE=Shoot;
RX PubMed=17350935; DOI=10.1093/jxb/erm016;
RA Tabuchi M., Abiko T., Yamaya T.;
RT "Assimilation of ammonium ions and reutilization of nitrogen in rice (Oryza
RT sativa L.).";
RL J. Exp. Bot. 58:2319-2327(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Involved in glutamate biosynthesis. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 L-glutamate + NAD(+) = 2-oxoglutarate + H(+) + L-glutamine +
CC NADH; Xref=Rhea:RHEA:13753, ChEBI:CHEBI:15378, ChEBI:CHEBI:16810,
CC ChEBI:CHEBI:29985, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC ChEBI:CHEBI:58359; EC=1.4.1.14;
CC -!- COFACTOR:
CC Name=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137; Evidence={ECO:0000250};
CC Note=Binds 1 [3Fe-4S] cluster. {ECO:0000250};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000250};
CC -!- PATHWAY: Amino-acid biosynthesis; L-glutamate biosynthesis via GLT
CC pathway; L-glutamate from 2-oxoglutarate and L-glutamine (NAD(+)
CC route): step 1/1.
CC -!- PATHWAY: Energy metabolism; nitrogen metabolism.
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in leaf blades and sheaths.
CC {ECO:0000269|PubMed:17350935}.
CC -!- SIMILARITY: Belongs to the glutamate synthase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAT58702.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes.; Evidence={ECO:0000305};
CC Sequence=AAT58740.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes.; Evidence={ECO:0000305};
CC Sequence=BAF18188.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB274818; BAF80064.1; -; mRNA.
DR EMBL; AC093956; AAT58740.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC104709; AAT58702.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008211; BAF18188.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014961; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_015640407.1; XM_015784921.1.
DR AlphaFoldDB; Q0DG35; -.
DR SMR; Q0DG35; -.
DR STRING; 4530.OS05T0555600-01; -.
DR PaxDb; Q0DG35; -.
DR PRIDE; Q0DG35; -.
DR GeneID; 4339561; -.
DR KEGG; osa:4339561; -.
DR eggNOG; KOG0399; Eukaryota.
DR HOGENOM; CLU_000422_3_0_1; -.
DR InParanoid; Q0DG35; -.
DR OrthoDB; 126283at2759; -.
DR PlantReactome; R-OSA-1119535; Glutamate biosynthesis IV.
DR UniPathway; UPA00045; -.
DR UniPathway; UPA00634; UER00690.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR Genevisible; Q0DG35; OS.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR GO; GO:0016040; F:glutamate synthase (NADH) activity; IBA:GO_Central.
DR GO; GO:0015930; F:glutamate synthase activity; IBA:GO_Central.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0019676; P:ammonia assimilation cycle; IBA:GO_Central.
DR GO; GO:0006537; P:glutamate biosynthetic process; IBA:GO_Central.
DR GO; GO:0097054; P:L-glutamate biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00982; gltB_C; 1.
DR CDD; cd02808; GltS_FMN; 1.
DR Gene3D; 1.10.1060.10; -; 1.
DR Gene3D; 2.160.20.60; -; 1.
DR Gene3D; 3.20.20.70; -; 2.
DR Gene3D; 3.50.50.60; -; 1.
DR Gene3D; 3.60.20.10; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR028261; DPD_II.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR017932; GATase_2_dom.
DR InterPro; IPR002489; Glu_synth_asu_C.
DR InterPro; IPR036485; Glu_synth_asu_C_sf.
DR InterPro; IPR006982; Glu_synth_centr_N.
DR InterPro; IPR012220; Glu_synth_euk.
DR InterPro; IPR002932; Glu_synthdom.
DR InterPro; IPR006005; Glut_synth_ssu1.
DR InterPro; IPR009051; Helical_ferredxn.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR Pfam; PF14691; Fer4_20; 1.
DR Pfam; PF00310; GATase_2; 1.
DR Pfam; PF04898; Glu_syn_central; 1.
DR Pfam; PF01645; Glu_synthase; 1.
DR Pfam; PF01493; GXGXG; 1.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR PIRSF; PIRSF000187; GOGAT; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR SUPFAM; SSF56235; SSF56235; 1.
DR SUPFAM; SSF69336; SSF69336; 1.
DR TIGRFAMs; TIGR01317; GOGAT_sm_gam; 1.
DR PROSITE; PS51278; GATASE_TYPE_2; 1.
PE 2: Evidence at transcript level;
KW 3Fe-4S; Amino-acid biosynthesis; Chloroplast; FAD; Flavoprotein; FMN;
KW Glutamate biosynthesis; Glutamine amidotransferase; Iron; Iron-sulfur;
KW Metal-binding; NAD; Oxidoreductase; Plastid; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..30
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 31..2188
FT /note="Glutamate synthase 2 [NADH], chloroplastic"
FT /id="PRO_0000395202"
FT DOMAIN 107..511
FT /note="Glutamine amidotransferase type-2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00609"
FT REGION 14..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 61..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 107
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00609"
FT BINDING 1198..1255
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250"
FT BINDING 1251
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000250"
FT BINDING 1257
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000250"
FT BINDING 1262
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000250"
FT BINDING 1974..1988
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000255"
SQ SEQUENCE 2188 AA; 239457 MW; 6ADE6929E0BBDD82 CRC64;
MSAAQGLALK LRAAPAAGGV RGEKRRRAAS ATAAAAARPR HGAMSLEGGF LGGALPAEDR
VAPRASASRQ AEAGAGAGAA RPPPRSMSKI PESSIGLYDP SMERDSCGVG FIAELSGEYS
RKTVDDAIEM LDRMAHRGAC GCEKNTGDGA GILVALPHNF FREVTKDAGF ELPPPGEYAV
GMFFMPTDDK RREKSKLLFR EKAELLGHTV LGWRRVPTDN SGLGQSAVDT EPVIEQVFVT
KSASSKADFE RQMYVLRRFS VMSIREVLGV KNGGTKDFYM CSLSSRTIVY KGQLKPSQLK
GYFFADLGDE SFTSYMALIH SRFSTNTFPS WDRAQPMRVL GHNGEINTLR GNKNWMKARE
GLLKCEGLGL TRDEMLKLLP IVDATSSDSG AIDNVLELLI QSGRSAPEAV MMMIPEAWQN
DVNMDPERKA LYEFFSALME PWDGPALISF TDGRYLGATL DRNGLRPGRF YVTYSGRVIM
ASEVGVVDVP PQDVSRKGRL NPGMMLLVDF ENHCVVNDDE LKKEYSKVRP YGEWLKRQRI
QLTDIIESVN EAERIAPSIS GALPITKENK ADMGICGILT PLKAFGYTRE ALEMLMLPMA
KDGQEALGSM GNDTPLAVMS NREKLTFEYF KQMFAQVTNP PIDPIREKIV TSMECMIGPE
GDLSETTERQ CHRLTLKSPL LNTNEMEAIK KMNYRGWRSK VLDITYPKKN GRMGLKQTLD
KICAQAREAI HEGYTILVLS DRGFSSERVA VSSLLAVGAV HQHLVSHLER TRIGLLVESA
EPREVHHFST LIGFGADAIC PYLAIEAIWR LQIDGRIPPN DGKPYTQEQL IEKYFYASNY
GMMKVLAKMG ISTLASYKGA QIFEALGLAS EVVSKCFEGT PSRVEGATFE MLAQDALRLH
EIAFPSRTLP PGSADANALP NPGDYHWRKN GEVHLNDPFS IAKLQEAARI NSREAYKEYS
RRIYELNKAC TLRGMLKFRE IPNQISLDEV EPAKEIVKRF CTGAMSYGSI SLEAHTSLAE
AMNTLGGKSN TGEGGEQPCR MVPLPDGSKN PRISAIKQVA SGRFGVSIYY LTNAVEVQIK
MAQGAKPGEG GELPGHKVIG DIAVTRNSTA GVGLISPPPH HDIYSIEDLA QLIHDLKNAN
PGARISVKLV SEAGVGIVAS GVVKGHADHV LISGHDGGTG ASRWTGIKNA GLPWELGLAE
THQTLVANGL RGRAVLQTDG QMKTGRDVAV ACLLGAEEFG FSTAPLITLG CIMMRKCHTN
TCPAGIATQD PVLRAKFAGK PEHVINYFFM LAEEVREIMA QLGFRTVNEM VGRSDMLEID
PKVLEGNEKL ENIDLSRLLK PAAEISPGAV QYCVEKQDHG LDMALDNKLI ASSTAALRKG
VRVFIETPVR NINRAVGTML SHEVTKRYHI HGLPSDTIHI KLNGSAGQSF GAFLCPGITL
ELEGDSNDYV GKGLSGGKIV VYPPRNSRFN PQDNIVIGNV ALYGATKGEA YFNGMAAERF
CVRNSGAQAV VEGIGDHGCE YMTGGTAVIL GKTGRNFAAG MSGGIAYVYD VDGKFSSRCN
YELVDLYAVV EEDDITTLRM MIQQHRLHTQ SDLARDILLN FDTLLPKFIK VYPRDYKRVL
DKLKEEKAAK EAEQKAREVV DKKPVEVIQA PNGISVKTEK VMNEEPSSRP SRVSNAVKYR
GFIKYEREGT SYRDPNERVK DWNEVAIELV PGPLLKTQSA RCMDCGTPFC HQEGSGAGCP
LGNKIPEFNE LVHQNRWHEA LDRLLETNNF PEFTGRVCPA PCEGSCVLGI IDNPVSIKSI
ECAIIDKGFE EGWMVPRPPL RRTGKRVAIV GSGPAGLAAA DQLNKMGHFV TVFERADRIG
GLMMYGVPNM KADKEGIVQR RVELMAKEGV QFIVNAHVGS DPLYSVEKLR SENDAIILAC
GATKPKDLPI PGRELAGIHF AMEFLHANTK SLLDSNLEDG NYISAQGRKV VVIGGGDTGT
DCIGTSIRHG CTNLVNLELL PEPPRKRAPD NPWPQWPRIF RVDYGHQEAT SKFGKDPRSY
KVLTKRFIGD ENGNVKALEV IRVEWGKVNG RFQFKEVEGS EEIIEADLVL LAMGFLGPEA
TVANKLGLEQ DMRSNFKAQF GNFATNVEGV FAAGDCRRGQ SLVVWAITEG RQAAAAVDNY
LSKDDEGETN GTEDIAVSSE GLVQPVVA
