GLT3_ARATH
ID GLT3_ARATH Reviewed; 227 AA.
AC Q9S772; Q1PFD3;
DT 29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Germin-like protein subfamily T member 3;
DE Flags: Precursor;
GN OrderedLocusNames=At1g74820; ORFNames=F25A4.21, F9E10.33;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
CC -!- FUNCTION: May play a role in plant defense. Probably has no oxalate
CC oxidase activity even if the active site is conserved.
CC -!- SUBUNIT: Oligomer (believed to be a pentamer but probably hexamer).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the germin family. {ECO:0000305}.
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DR EMBL; AC008263; AAD55294.1; -; Genomic_DNA.
DR EMBL; AC013258; AAG51910.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35635.1; -; Genomic_DNA.
DR EMBL; DQ446430; ABE65773.1; -; mRNA.
DR PIR; F96777; F96777.
DR RefSeq; NP_177620.1; NM_106140.3.
DR AlphaFoldDB; Q9S772; -.
DR SMR; Q9S772; -.
DR STRING; 3702.AT1G74820.1; -.
DR PaxDb; Q9S772; -.
DR PRIDE; Q9S772; -.
DR EnsemblPlants; AT1G74820.1; AT1G74820.1; AT1G74820.
DR GeneID; 843821; -.
DR Gramene; AT1G74820.1; AT1G74820.1; AT1G74820.
DR KEGG; ath:AT1G74820; -.
DR Araport; AT1G74820; -.
DR TAIR; locus:2027232; AT1G74820.
DR eggNOG; ENOG502QSRM; Eukaryota.
DR HOGENOM; CLU_015790_0_3_1; -.
DR InParanoid; Q9S772; -.
DR OMA; MAHISQI; -.
DR OrthoDB; 1164277at2759; -.
DR PhylomeDB; Q9S772; -.
DR PRO; PR:Q9S772; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9S772; baseline and differential.
DR Genevisible; Q9S772; AT.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR006045; Cupin_1.
DR InterPro; IPR001929; Germin.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR Pfam; PF00190; Cupin_1; 1.
DR PRINTS; PR00325; GERMIN.
DR SMART; SM00835; Cupin_1; 1.
DR SUPFAM; SSF51182; SSF51182; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Disulfide bond; Glycoprotein; Manganese; Metal-binding;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..227
FT /note="Germin-like protein subfamily T member 3"
FT /id="PRO_0000010832"
FT DOMAIN 71..219
FT /note="Cupin type-1"
FT /evidence="ECO:0000255"
FT BINDING 119
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 121
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 126
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 165
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT CARBOHYD 78
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 143
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 44..59
FT /evidence="ECO:0000250"
SQ SEQUENCE 227 AA; 24127 MW; C64025B5FD4BCCCF CRC64;
MAHISQISSF LSIVLIFLAL CITLFTNPTL SLPALKLNPF QDFCVADLQA TPTNSGYPCK
SQVTSEDFFY SGLNTPLNTS NPKGIAANPA NLLTFPGLNT LGISMYNVAI APGGYNQPHS
HPGVTEAGVV IEGSVLVGFL TTNYTLYSKV IGPGDMFVIP PGLIHYEGNV GKTQCRLLTV
VADDLPSEVG VPHTLLATKP AIPNEVLISA FKADSKTINM LRSKFTA
