GLTB_NEOYE
ID GLTB_NEOYE Reviewed; 1538 AA.
AC Q1XDB2;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 02-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Ferredoxin-dependent glutamate synthase;
DE EC=1.4.7.1;
DE AltName: Full=Fd-GOGAT;
GN Name=gltB;
OS Neopyropia yezoensis (Susabi-nori) (Pyropia yezoensis).
OG Plastid; Chloroplast.
OC Eukaryota; Rhodophyta; Bangiophyceae; Bangiales; Bangiaceae; Neopyropia.
OX NCBI_TaxID=2788;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=U-51;
RA Kunimoto M., Morishima K., Yoshikawa M., Fukuda S., Kobayashi T.,
RA Kobayashi M., Okazaki T., Ohara I., Nakayama I.;
RT "Whole genome sequence of Porphyra yezoensis chloroplast.";
RL Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 L-glutamate + 2 oxidized [2Fe-2S]-[ferredoxin] = 2-
CC oxoglutarate + 2 H(+) + L-glutamine + 2 reduced [2Fe-2S]-
CC [ferredoxin]; Xref=Rhea:RHEA:12128, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC COMP:10001, ChEBI:CHEBI:15378, ChEBI:CHEBI:16810, ChEBI:CHEBI:29985,
CC ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:58359; EC=1.4.7.1;
CC -!- COFACTOR:
CC Name=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137; Evidence={ECO:0000250};
CC Note=Binds 1 [3Fe-4S] cluster. {ECO:0000250};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000250};
CC -!- PATHWAY: Amino-acid biosynthesis; L-glutamate biosynthesis via GLT
CC pathway; L-glutamate from 2-oxoglutarate and L-glutamine (ferredoxin
CC route): step 1/1.
CC -!- PATHWAY: Energy metabolism; nitrogen metabolism.
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma.
CC -!- SIMILARITY: Belongs to the glutamate synthase family. {ECO:0000305}.
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DR EMBL; AP006715; BAE92499.1; -; Genomic_DNA.
DR RefSeq; YP_537056.1; NC_007932.1.
DR AlphaFoldDB; Q1XDB2; -.
DR SMR; Q1XDB2; -.
DR PRIDE; Q1XDB2; -.
DR GeneID; 3978887; -.
DR UniPathway; UPA00045; -.
DR UniPathway; UPA00634; UER00691.
DR GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0016041; F:glutamate synthase (ferredoxin) activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0097054; P:L-glutamate biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00982; gltB_C; 1.
DR CDD; cd02808; GltS_FMN; 1.
DR Gene3D; 2.160.20.60; -; 1.
DR Gene3D; 3.20.20.70; -; 2.
DR Gene3D; 3.60.20.10; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR017932; GATase_2_dom.
DR InterPro; IPR002489; Glu_synth_asu_C.
DR InterPro; IPR036485; Glu_synth_asu_C_sf.
DR InterPro; IPR006982; Glu_synth_centr_N.
DR InterPro; IPR002932; Glu_synthdom.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR Pfam; PF00310; GATase_2; 1.
DR Pfam; PF04898; Glu_syn_central; 1.
DR Pfam; PF01645; Glu_synthase; 1.
DR Pfam; PF01493; GXGXG; 1.
DR SUPFAM; SSF56235; SSF56235; 1.
DR SUPFAM; SSF69336; SSF69336; 1.
DR PROSITE; PS51278; GATASE_TYPE_2; 1.
PE 3: Inferred from homology;
KW 3Fe-4S; Amino-acid biosynthesis; Chloroplast; FAD; Flavoprotein; FMN;
KW Glutamate biosynthesis; Glutamine amidotransferase; Iron; Iron-sulfur;
KW Metal-binding; Oxidoreductase; Plastid.
FT CHAIN 1..1538
FT /note="Ferredoxin-dependent glutamate synthase"
FT /id="PRO_0000277304"
FT DOMAIN 34..431
FT /note="Glutamine amidotransferase type-2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00609"
FT ACT_SITE 34
FT /note="For GATase activity"
FT /evidence="ECO:0000250"
FT BINDING 1109..1166
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250"
FT BINDING 1162
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000250"
FT BINDING 1168
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000250"
FT BINDING 1173
FT /ligand="[3Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:21137"
FT /evidence="ECO:0000250"
SQ SEQUENCE 1538 AA; 168811 MW; 5993783A58126647 CRC64;
MFNQKIVDQA PRKLTSRLTN SSSLISIEKE RDACGVGFIA DVNNVANHKI VVQALEALTC
MEHRGACSAD RDSGDGAGIT TAIPWNLFQK SLQNQNIKFE QNDSVGVGML FLPAHKLKES
KLIIETVLKE ENLEIIGWRL VPTVQEVLGK QAYLNKPHVE QVFCKSSNLS KDRLEQQLFL
VRKKIEKYIG INGKDWAHEF YICSLSCYTI VYKGMMRSAV LGQFYQDLYH SEYTSSFAIY
HRRFSTNTMP KWPLAQPMRF VSHNGEINTL LGNLNWMQSR EPLLQSKVWK DRIHELKPIT
NKDNSDSANL DAAVELLIAS GRSPEEALMI LVPEAFQNQP DFANNTEISD FYEYYSGLQE
PWDGPALVVF TNGKVIGATL DRNGLRPARY VITKDNLVIV SSESGVVQVE PGNVKSKGRL
GPGQMISVDI FSHKILNNKE IKTSVTTKIP YGELLTDARQ ILEHKPFLSD QQVDIKKLMQ
LQTAFGYTNE DVELVIEHMA SQAKEPTFCM GDDIPLSILS EKSHILYDYF KQRFAQVTNP
AIDPLRESLV MSLAIQIGHK SNLLDDQPTL AKHIKLESPV INEGELNAIF ESKLSCIRIN
TLFQLEDGPK NFKQQIQQLC ENASQAILDG NNILVLSDKN NSLDSEKVSI PPLLAVGAVH
HHLINKGLRQ EASILVETAQ CWSTHHFACL IGYGASAICP YLAFETARHW WSNPKTKMLM
SKGRLPACNI QEAQANYKKA VEAGLLKILS KMGISLLSSY HGAQIFEILG LGSEVVNLAF
KGTTSQIGGL SMIELGRETV NIHSKAFSEV KTKKLANYGF VQYRPGGEYH INNPEMSKAL
HQAVRGYNPE YYNNYQSLLQ NRPPTALRDL LKLQSNRAPI SIDEVESIED ILQKFCTGGM
SLGALSRETH ETLAIAMNRI GGKSNSGEGG EDPVRFKILN DVNSSGTSPL LPHLKGLKNG
DTASSAIKQI ASGRFGVTPE YLMNAKQLEI KIAQGAKPGE GGQLPGKKIS PYIATLRKCK
PGVPLISPPP HHDIYSIEDL SQLIFDLHQI NPKAKISVKL VSEIGIGTIA AGVAKGNADI
IQISGHDGGT GASPLSSIKH AGSPWELGLS EVHQLLAENQ LRDRVTLRVD GGLRTGSDIV
LAAIMGAEEF GFGTVAMIAT GCIMARICHT NKCPVGVATQ REELRARFSG VPEALVNFFL
FIGNEVREIL ASLGYKSLDD ITGQNHLLIK NTDINLTKTN GIQLDTLINI NNNTWTKFNS
VHTNGPVMDD DILAIPEVSN AIKLETEITK HFKIANTNRT VGTRLSGIIA KNYGNTGFKG
LIKLNFYGSA GQSFGAFLAS GINLKLMGEA NDYVGKGMNG GSIVIVPPAG TIYEDNNQVI
IGNTCLYGAT GGYLFAQGQA GERFAVRNSL AESVVEGVGD HACEYMTGGV IVVLGKAGRN
VGAGMTGGLA YFLDEDENFI DRVNSEIVKI QRVITKAGEE QLKNLIENHA AKTGSLKAHT
ILEKWNSYLP QFWQVVPPSE ANIDETNPTY SSNTIAAC
